Viabilidade da aplicação do método respirométrico de Bartha para determinação da biodegradação de poluentes ou resíduos em latossolos

Abstract

SIDNEIDE MANFREDINIEngenheira agrônoma pela Faculdade de Agronomia de Jaboticabal -UNESP. Mestre em energia nuclear na agricultura pelo CENA-ESALQ -USP. Doutora em solos e nutrição mineral de plantas pela ESALQ-USP. Docente do ABSTRACTOne of the major parameters that influence the feasibility of using bioremediation in contaminated sites is the biodegradability of the compounds of concern. The Bartha´s respirometric method, adapted from a Dutch norm, is a common method used in Brazil to determine such biodegradability. However, tropical soils present different characteristics as compared to soils from regions of temperate climate. In this research, its applicability was studied for a latosoil, which is the predominant soil type in São Paulo State, Brazil. Based on the results of this study, it can be concluded that abiotic reactions in latosoil generated carbonic gas in significant concentrations. Furthermore, this research verified the difficulty of sterilizing the soil, which would make it difficult to evaluate the removal of contaminants by other mechanisms or the biodegradation by exogenous microorganisms. Therefore, the application of Bartha's respirometric method is not recommended to determine the biodegradability of pollutants in latosoils.

Bioprospection of bacteria and yeasts from Atlantic Rainforest soil capable of growing in crude-glycerol residues

“…A standardized solution of 0.1 N HCl was used to titrate KOH. From the volume of acid added during titration, the release of CO 2 was quantified using the formula: (1) where: A = volume of 0.1 N HCl used to titrate the KOH solution in the blank test (mL); B = volume of 0.1 N HCl used to titrate the KOH solution in each treatment (mL); 50 = factor to convert the volume of CO 2 to micromoles (µmol); f HCl = factor of the 0.1 N HCl solution (Mello et al, 2007).…”

“…In this context, the Bartha respirometer (Bartha and Pramer, 1965) is a simple method that is frequently used in measurements of microbial activity in soil microcosms (ABNT, 1999;Mello et al, 2007). In addition, once the existence of active microbes in a given environment is established, subsequent biotechnological applications depend on the possibility of isolating and cultivating these microorganisms in vitro.…”