The use of polymerase chain reaction to confirm diagnosis in skin biopsies consistent with american tegumentary leishmaniasis at histopathology: a study of 90 cases

Andrade, Rosilene Viana de; Massone, Cesare; Lucena, Meline Nogueira Barbosa de; Talhari, Anette Chusciak; Talhari, Sinésio; Guerra, Jorge Augusto de Oliveira; Ferreira, Luíz Carlos de Lima

doi:10.1590/s0365-05962011000500005

Cited by 17 publications

(10 citation statements)

References 14 publications

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“…The higher efficiency of the kDNA-PCR method over the traditional methods for ATL diagnosis (Table 1) observed here is in agreement with the literature, which describes sensitivities that range from 75% - 98%, and it is attributed to the naturally amplified DNA in the kinetoplast minicircle 2 4 5 6 14 17 20 30 34 35 40 .…”

Section: Discussionsupporting

confidence: 92%

“…Although kDNA- Hae III PCR-RFLP reaction can contribute to the identification of L. (V.) braziliensis infected samples, the confirmation of the species of the parasite by other molecular methods is recommended, once similar electrophoretic patterns may occur within related species from Viannia subgenus as reported in other PCR-RFLP studies 2 38 39 .…”

Section: Discussionmentioning

confidence: 99%

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APPLICABILITY OF kDNA-PCR FOR ROUTINE DIAGNOSIS OF AMERICAN TEGUMENTARY LEISHMANIASIS IN A TERTIARY REFERENCE HOSPITAL

Satow

Yamashiro-Kanashiro

Rocha

et al. 2013

Rev. Inst. Med. trop. S. Paulo

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SUMMARYThis study evaluated the applicability of kDNA-PCR as a prospective routine diagnosis method for American tegumentary leishmaniasis (ATL) in patients from the Instituto de Infectologia Emílio Ribas (IIER), a reference center for infectious diseases in São Paulo - SP, Brazil. The kDNA-PCR method detected Leishmania DNA in 87.5% (112/128) of the clinically suspected ATL patients, while the traditional methods demonstrated the following percentages of positivity: 62.8% (49/78) for the Montenegro skin test, 61.8% (47/76) for direct investigation, and 19.3% (22/114) for in vitro culture. The molecular method was able to confirm the disease in samples considered negative or inconclusive by traditional laboratory methods, contributing to the final clinical diagnosis and therapy of ATL in this hospital. Thus, we strongly recommend the inclusion of kDNA-PCR amplification as an alternative diagnostic method for ATL, suggesting a new algorithm routine to be followed to help the diagnosis and treatment of ATL in IIER.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

APPLICABILITY OF kDNA-PCR FOR ROUTINE DIAGNOSIS OF AMERICAN TEGUMENTARY LEISHMANIASIS IN A TERTIARY REFERENCE HOSPITAL

Satow

Yamashiro-Kanashiro

Rocha

et al. 2013

Rev. Inst. Med. trop. S. Paulo

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“…However, the kDNA-PCR method was positive in 19 patients, indicating that L. (V.) braziliensis is the predominant species, although similar electrophoretic patterns may occur within related species from Viannia subgenus, as some studies have reported. [17][18][19] Other species can be important in specific geographical situations, and molecular methods play a pivotal role in defining different clinical presentations, response to treatment, and choice of the best drug for each case. Guerra and others reported 46 cases of ML along the Amazon region, with 16 cases caused by L. (V.) guyanensis, thus showing that this species is also a significant causative agent within the region.…”

Section: Discussionmentioning

confidence: 99%

Efficacy and Safety of Liposomal Amphotericin B for the Treatment of Mucosal Leishmaniasis from the New World: A Retrospective Study

Cunha¹,

Leão²,

Soler³

et al. 2015

The American Journal of Tropical Medicine and Hygiene

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Abstract. The standard treatment of mucosal leishmaniasis (ML) is pentavalent antimonials, agents with serious adverse effects. Alternative agents include amphotericin B deoxycholate and liposomal amphotericin B. We performed a retrospective study including 29 patients treated with liposomal amphotericin B, most of whom had comorbidities, history of previous treatment of ML, and contraindications to the use of antimonial pentavalent or amphotericin B deoxycholate. We observed a cure rate of 93.1%. Kidney failure was the most important side effect, reported in five patients (17.2%). This study showed a good efficacy and safety profile of liposomal amphotericin B in patients with ML and contraindications to the use of other agents.

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“…This shows the high sensitivity of PCR in cases in which histopathology is compatible with ACL. 16 This is confirmed by Benicio et al (2011), who evaluated diagnostic methods in an endemic area for Leishmania (Leishmania) amazonensis in the Brazilian Amazon. The authors studied 180 patients and conducted skin biopsy, histological examination, PCR and culture.…”

Section: Discussionmentioning

confidence: 75%

American cutaneous leishmaniasis: epidemiological profile of patients treated in Londrina from 1998 to 2009

Pontello

Gon

Ogama

2013

An. Bras. Dermatol.

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BACKGROUND American cutaneous leishmaniasis is a dermatozoonosis of compulsory notification with relevant morbidity. The disease is considered endemic in northern Paraná. OBJECTIVES To evaluate the clinical, laboratory and epidemiological aspects of patients with American cutaneous leishmaniasis treated at Clinics Hospital, State University of Londrina, Brazil (Paraná). METHODSThis was an observational, cross-sectional, retrospective and descriptive study. The medical records of patients with American cutaneous leishmaniasis treated at the University Hospital of Londrina - Paraná, from 1998 to 2009 were evaluated. RESULTS A total of 470 patients, with prevalence of 8.72 cases / 100,000 inhabitants, participated in the study. Most patients were male, aged 21-40 years, with a single ulcerated lesion as the most common clinical presentation, located mainly in an exposed area of the body. With regard to immunological tests, the Montenegro intradermal reaction was positive in 84.4% of cases. Treatment with pentavalent antimony was well tolerated, and patient follow-up for a year occurred in 59% of cases. CONCLUSIONAmerican cutaneous leishmaniasis is still an endemic disease in the region, with a high potential for morbidity, but with a cure rate of around 95% after treatment. The use of immunological techniques facilitates the diagnosis of clinically suspicious cases.

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The use of polymerase chain reaction to confirm diagnosis in skin biopsies consistent with american tegumentary leishmaniasis at histopathology: a study of 90 cases

Abstract: Polymerase chain reaction performed by amplifying kDNA is able to confirm a diagnosis of cutaneous leishmaniasis with a high degree of sensitivity in cases in which histopathology is consistent with a diagnosis of cutaneous leishmaniasis but not definitive.

Cited by 17 publications

References 14 publications

APPLICABILITY OF kDNA-PCR FOR ROUTINE DIAGNOSIS OF AMERICAN TEGUMENTARY LEISHMANIASIS IN A TERTIARY REFERENCE HOSPITAL

APPLICABILITY OF kDNA-PCR FOR ROUTINE DIAGNOSIS OF AMERICAN TEGUMENTARY LEISHMANIASIS IN A TERTIARY REFERENCE HOSPITAL

Efficacy and Safety of Liposomal Amphotericin B for the Treatment of Mucosal Leishmaniasis from the New World: A Retrospective Study

American cutaneous leishmaniasis: epidemiological profile of patients treated in Londrina from 1998 to 2009

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