Biochemical and Immunological Characterization of the Main Products of Crotoxin Irradiation

Nascimento, N.

doi:10.1590/s0104-79301996000200015

Cited by 2 publications

(9 citation statements)

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“…This agrees with the findings of other authors (7,12,21,(31)(32)(33)(34)(35)(36)(37)(38)42), which assured that ionizing radiation has the property to attenuate venoms toxicity without altering immunological properties.…”

Section: Discussionsupporting

confidence: 92%

“…This method has showed to present the best specificity, sensitivity, fastness, simplicity, and low costs (3,4,6,32,35,39,44). Results showed that the optical density values obtained in the ELISA test for the several dilutions of the native and irradiated venoms of Bothrops jararaca, Bothrops jararacussu and Bothrops moojeni did not show statistical difference between the groups studied.…”

Section: Discussionmentioning

confidence: 99%

“…Some authors (12,32,33) verified that, after irradiation, Crotalus durissus terrificus venom and its fractions antigenic and immunogenic properties were preserved and toxicity was reduced.…”

Section: Discussionmentioning

confidence: 99%

“…This way, 2000Gy irradiation is the ideal dose for snake venoms detoxification, since it preserves many immunological properties (31)(32)(33).…”

Section: Discussionmentioning

confidence: 99%

“…The principal physical agents are: x-ray radiation (19), ultraviolet radiation (46), heat (15), and gamma radiation (25). The latter, according to previous works (1,7,9,12,14,17,21,(31)(32)(33)(34)(35)(36)(37)(38)(41)(42)(43), has proved to be an excellent tool of detoxification, since it is able to reduce toxicity without altering immunogenicity. In addition, this radiation does not add any new substance to the venom.…”

Section: Introductionmentioning

confidence: 92%

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Immunological assessment of mice hyperimmunized with native and Cobalt-60-irradiated Bothrops venoms

Ferreira¹,

Nascimento²,

Martı́nez

et al. 2005

J. Venom. Anim. Toxins incl. Trop. Dis

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ELISA was used to evaluate, accompany, and compare the humoral immune response of Swiss mice during hyperimmunization with native and Cobalt-60-irradiated ( 60 Co) venoms of Bothrops jararaca, Bothrops jararacussu and Bothrops moojeni. Potency and neutralization were evaluated by in vitro challenges. After hyperimmunization, immunity was observed by in vivo challenge, and the side effects were assessed. The animals immunization with one LD 50 of each venom occurred on days 1, 15, 21, 30, and 45, when blood samples were collected; challenges happened on the 60 th day. Results showed that ELISA was efficient in evaluating, accompanying and comparing mouse immune response during hyperimmunization. Serum titers produced with natural venom were similar to those produced with irradiated venom. Immunogenic capacity was maintained after 60 Coirradiation. The sera produced with native venom showed neutralizing potency and capacity similar to those of the sera produced with irradiated venom. All antibodies were able to neutralize five LD 50 from these venoms. Clinical alterations were minimum during hyperimmunization with irradiated venom, however, necrosis and death occurred in animals inoculated with native venom.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…This way, 2000Gy irradiation is the ideal dose for snake venoms detoxification, since it preserves many immunological properties (31)(32)(33).…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 92%

See 3 more Smart Citations

Immunological assessment of mice hyperimmunized with native and Cobalt-60-irradiated Bothrops venoms

Ferreira¹,

Nascimento²,

Martı́nez

et al. 2005

J. Venom. Anim. Toxins incl. Trop. Dis

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Immunization with native and cobalt 60-irradiated Crotalus durissus terrificus venom in swiss mice: assessment of the neutralizing potency of antisera

Ferreira

Nascimento

Martı́nez

et al. 2005

J. Venom. Anim. Toxins incl. Trop. Dis

Self Cite

View full text Add to dashboard Cite

ELISA was used to evaluate, follow, and compare the humoral immune response of Swiss mice during hyperimmunization with natural and Cobalt 60-irradiated (60Co) Crotalus durissus terrificus venom. Potency and neutralization were evaluated by in vitro challenges. After hyperimmunization, immunity was observed by "in vivo" challenge and the side effects were assessed. The animals immunization with one LD50 of the venom was on days one, 15, 21, 30, and 45, when blood samples were collected; the challenges occurred on the 60th day. Results showed that ELISA was efficient in evaluating, following, and comparing mouse immune response during hyperimmunization. Serum titers produced with natural venom were similar to those produced with irradiated venom. Immunogenic capacity was maintained after 60Co irradiation. Serum produced from Crotalus durissus terrificus irradiated venom showed higher potency and neutralization capacity than that from natural venom. All antibodies were able to neutralize five LD50 from these venoms. Clinical alterations were minimum during hyperimmunization with irradiated venom

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Biochemical and Immunological Characterization of the Main Products of Crotoxin Irradiation

Cited by 2 publications

References 0 publications

Immunological assessment of mice hyperimmunized with native and Cobalt-60-irradiated Bothrops venoms

Immunological assessment of mice hyperimmunized with native and Cobalt-60-irradiated Bothrops venoms

Immunization with native and cobalt 60-irradiated Crotalus durissus terrificus venom in swiss mice: assessment of the neutralizing potency of antisera

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