Kinetic study of the enzymatic hydrolysis of sugarcane bagasse

Carvalho, M. L.; Sousa, Ruy; Rodríguez-Zúñiga, Úrsula Fabiola; Suarez, Carlos Alberto Galeano; Rodrigues, Dilmer; Giordano, Roberto

doi:10.1590/s0104-66322013000300002

Cited by 44 publications

(32 citation statements)

References 20 publications

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“…The cellulose conversion results are in accordance with literature (Barcelos et al, 2011;Macedo et al, 2011;Carvalho et al, 2013;Idrees et al, 2014). Even working only with cellulases and β-glucosidase enzymes without xylanases or hemicellulase enzyme supplementation, it was possible to produce a high quantity of xylose in the enzymatic hydrolysis using the bagasse subjected to the alkaline pretreatment.…”

Section: Resultssupporting

confidence: 88%

Evaluation of Composition, Characterization and Enzymatic Hydrolysis of Pretreated Sugar Cane Bagasse

Guilherme

Dantas

Santos

et al. 2015

Braz. J. Chem. Eng.

156

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-Glucose production from sugarcane bagasse was investigated. Sugarcane bagasse was pretreated by four different methods: combined acid and alkaline, combined hydrothermal and alkaline, alkaline, and peroxide pretreatment. The raw material and the solid fraction of the pretreated bagasse were characterized according to the composition, SEM, X-ray and FTIR analysis. Glucose production after enzymatic hydrolysis of the pretreated bagasse was also evaluated. All these results were used to develop relationships between these parameters to understand better and improve this process. The results showed that the alkaline pretreatment, using sodium hydroxide, was able to reduce the amount of lignin in the sugarcane bagasse, leading to a better performance in glucose production after the pretreatment process and enzymatic hydrolysis. A good xylose production was also observed.

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Section: Resultssupporting

confidence: 88%

Evaluation of Composition, Characterization and Enzymatic Hydrolysis of Pretreated Sugar Cane Bagasse

Guilherme

Dantas

Santos

et al. 2015

Braz. J. Chem. Eng.

156

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“…The evolution of the fine population will be scrutinized deeply following number-weighted distributions using FBRM (see §3. 4…”

Section: Particle Size Distribution During Enzymatic Hydrolysismentioning

confidence: 99%

“…This resource is an abundant source of raw material for biotransformation. Thus, the conversion of sugarcane bagasse to fuel ethanol has long been under consideration [4][5][6]. However, the processes proposed in these publications remain in laboratory scale and need to be optimized for industrial application.…”

Section: Introductionmentioning

confidence: 99%

Using in-situ viscosimetry and morphogranulometry to explore hydrolysis mechanisms of filter paper and pretreated sugarcane bagasse under semi-dilute suspensions

Anne-Archard

Coma

Cameleyre

et al. 2017

Biochemical Engineering Journal

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OATAO is an open access repository that collects the work of Toulouse researchers and makes it freely available over the web where possible. This is an author-deposited version published in : http://oatao.univ-toulouse.fr/ Eprints ID : 18375 a b s t r a c tThe relationship between in-situ viscosimetry, in-and ex-situ morphogranulometry, and biochemistry has been investigated during enzymatic hydrolysis of pretreated sugarcane bagasse (SCB, 3% w/v) and filter paper (FP, 1.5% w/v) at various enzyme dosages (0.3-25 FPU/g cellulose). Semi-dilute conditions (1.5-2 times higher than critical concentrations) were considered in order to generate non-Newtonian rheological behaviors and to avoid neglecting particle-particle interactions in opposition to dilute conditions. Observed phenomena as well as hydrolysis mechanisms including solvation, separation, fragmentation, and solubilization appeared to depend strongly on initial substrate properties. For both FP and SCB, suspension viscosities were correlated with particle size distributions and volume fractions during hydrolysis, but they exhibited strongly different trends. With SCB, a viscosity overtaking was clearly observed at enzyme loading ≤10 FPU/g cellulose. This phenomenon is explained by the separation of agglomerates into individual fragments, leading to an increase in the total number of particles and a morphological shift of particles from sphere-like into fiber-like. With FP, the viscosity collapsed at the initial stage in relation to the volume reduction of coarse particles and the number increase of fine particles. Suspension viscosity was strongly dependent on the fraction of coarse population and nearly independent from the glucose conversion yield.Abbreviations: CLD, chord length distribution; DLS, diffraction light scattering; d SE , diameter of circle equivalent (m); FBRM, focused beam reflectance measurement; FP, filter paper; FPU, filter paper unit; lc , chord length (m); LOD, limit of detection; LOQ, limit of quantification; MO, Metzner-Otto concept; Nc, number of chord counted (/); Np, power number (/); PP, paper pulp; PSD, particle size distribution; Re, Reynolds number (/); SCB, sugarcane bagasse.

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“…The pretreatment of lignocellulose to obtain fermentable sugars is an essential step for lignocellulose conversion with microbial fermentation. A variety of pretreatment methods using mechanical, chemical and biological processing have been developed to change the structural and chemical composition of lignocellulose in order to improve sugar yields (Carvalho et al, 2013;Duangwang and Sangwichien, 2013;Liu and Wang, 2016). Among these methods, an acid hydrolysis pretreatment can be done with concentrated or dilutedacid, but the use of concentrated acids is less desirable because inhibitory compounds are formed.…”

Section: Introductionmentioning

confidence: 99%

Xylose recovery from dilute-acid hydrolysis of oil palm (Elaeis guineensis) empty fruit bunches for xylitol production

Manjarrés-Pinzón¹,

Arias-Zabala²,

Londoño³

et al. 2017

Afr. J. Biotechnol.

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The aim of this study was to evaluate the effect of different process conditions, such as the sulfuric acid concentration, contact time, solid:liquid ratio and particle size, on the xylose recovery and the formation of by-products from oil palm empty fruit bunch (OPEFB) with dilute-acid hydrolysis. Moreover, an adapted and non-adapted strain of the yeast Candida guilliermondii was used to obtain xylitol from the optimal hydrolysate. Xylose, glucose, hydroxymethylfurfural (HMF) and acetic acid in the acid hydrolysate were analyzed with high performance liquid chromatography (HPLC). A BoxBehnken-based design was used to find the combination of factors that maximized the formation of xylose in the hydrolysate optimization process. The fermentation to obtain xylitol from the optimized hydrolysate of OPEFB with adapted and non-adapted C. guilliermondii strains was made in 100 ml Erlenmeyer flasks at 30°C for 96 h at 200 rpm in an incubator shaker. The maximum xylose concentration (32.59 g L -1) was obtained at 121°C, for 30 min, with a 1:8 solid:liquid ratio, 2% acid concentration and particle size of around 4 cm. With the same conditions, the inhibitor concentrations, such as HMF, glucose and acetic acid, were 0.023, 1.033 and 11.078 g L -1, respectively. The optimized conditions were the same as previously described. The higher xylitol productivity (10.3 g L -1) and yield (0.43 g g -1) were obtained by fermentation with non-adapted C. guilliermondii strains from the optimized hydrolysate of OPEFB without the need for detoxification. It is not necessary to make an adaptation of C. guilliermondii in the optimized hydrolysate of OPEFB to produce xylitol.

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Kinetic study of the enzymatic hydrolysis of sugarcane bagasse

Cited by 44 publications

References 20 publications

Evaluation of Composition, Characterization and Enzymatic Hydrolysis of Pretreated Sugar Cane Bagasse

Evaluation of Composition, Characterization and Enzymatic Hydrolysis of Pretreated Sugar Cane Bagasse

Using in-situ viscosimetry and morphogranulometry to explore hydrolysis mechanisms of filter paper and pretreated sugarcane bagasse under semi-dilute suspensions

Xylose recovery from dilute-acid hydrolysis of oil palm (Elaeis guineensis) empty fruit bunches for xylitol production

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