Statistical optimization of medium compositions for chitosanase production by a newly isolated Streptomyces albus

Abstract

-The nutritional medium compositions required for chitosanase production by Streptomyces albus YT2 were optimized, and the strain was isolated from the coastal soil of Bohai Sea. Plackett-Burman design was applied to evaluate the effect of different medium compositions obtained by one-factor-at-a-time experiments. Glucose, peptone and MgSO 4 were found to influence the activity of chitosanase significantly. The steepest ascent method was employed to approach the experimental design space, followed by the use of Box-Behnken design and response surface methodology for further optimization of the chitosanase production. The medium composition for optimal production of chitosanase was as follows: 0.5 g/L chitosan, 55 g/L glucose, 1 g/L peptone, 2 g/L (NH 4 ) 2 SO 4 , 8 g/L urea, 1 g/L NH 4 Cl, 3 g/L KH 2 PO 4 , 1 g/L FeSO 4 ·7H 2 O, 2 g/L ZnSO 4 ·7H 2 O, 2 g/L CaCl 2 ·6H 2 O, 2 g/L MnSO 4 ·H 2 O and 2 g/L NaCl. The chitosanase production was found to increase from 11.56 U/mL to 39.87 U/mL, a 3.47-fold increase compared with that using the original medium.

Optimization of Alkaline Protease Production by Alkaliphilic Bacillus sp. KW2 in Low Cost Medium using Statistical Approaches

“…Similar fold increase in protease production after optimization by statistical methods were reported earlier for Bacillus mojavensis (4.2 fold) [34], Microbacterium sp. (3.6 fold) [12] and Bacillus amyloliquefaciens (3.92 fold) [35]. However, higher fold increase in protease production have also been reported for Bacillus sp.…”

STATISTICAL OPTIMIZATION OF MINERAL SALT AND UREA CONCENTRATION FOR CELLULASE AND XYLANASE PRODUCTION BY Penicillium echinulatum IN SUBMERGED FERMENTATION

“…Penicillium echinulatum has been identified as a potential candidate for cellulase and xylanase production because its secreting capacity is almost equivalent to the best T. reesei strains (Camassola and Dillon, 2010;Dillon et al, 2006). Statistical methods have also been designed for bioprocess optimization (Cheng et al, 2012;Coelho et al, 2011;Singh and Kaur, 2012). Combinatorial interactions of process variables with the production of the desired compound are numerous and the optimum processes may be developed using an effective experimental design procedure (Muthuvelayudham and Viruthagiri, 2010).…”

Production and Characterization of Partially Purified Chitosanase Enzyme of <i>Bacillus cereus</i> A4/B4 Isolated from Biowaste Soil Samples:Bioactive Chitooligosaccharide

“…From the suspension 0.1ml was spread plated on to the CDA [6] plates having basal medium M9 along with (0.5% ,1% ,2%) substrate chitosan or colloidal chitosan of 0.5% and 1% [7][8][9], Minimal Salt Chitosan (MS) medium [10,11], Minimal Synthetic (MSM) Medium [12] and all the plates were incubated at 30° C for 3-5 days.…”

“…The CDA broth was taken at various pH (4,5,6,7,8) and the selected strains was inoculated and were incubated at room temperature for 8 days. Every 24 hours, the enzyme activity was measured.…”

“…but no growth was observed in the present study (Figure 1). The chitosanase producing organism was isolated from soil sample forming a clear zone of halos on 1% colloidal chitosan medium [8,9] and 2% colloidal chitosan [7] whereas no growth was observed in M9 medium with 0.5% and 1%colloidal chitosan medium, Minimal salt (MS) medium along with 0.5% chitosan, Minimal Synthetic medium and Enriched medium.…”