Comparison between continuous and batch processing to produce xylanase by penicillium canescens 10-10c

Abstract

-Penicillium canescens 10-10c strain was cultivated on barley straw hydrolysate as a soluble nutrient source and as inducer for xylanase production. Barley straw hydrolysate was obtained by treatment of barley straw with NaOH or hot water. In shake flask cultures, NaOH treatment was found to increase the biomass production, but was not accompanied by an increase in xylanase production. The best xylanase production (54 U/ml) was observed on hydrolyzed extract from barley straw treated with hot water (100 ºC) for 3 hours. Enzyme production was further improved by scaling up the cultivation process to a 3-L stirred tank bioreactor. For batch cultivations in the bioreactor, the maximum xylanase productivity reached 1.31 and 0.46 U/ml/h, respectively, after 96 and 168 hours of cultivation. However, xylanase productivity reached 3.46 U/ml/h in the continuous culture. These results suggest that xylanase can be produced efficiently by Penicillium canescens 10-10c in continuous culture from an inexpensive source such as barley straw hydrolysate.

Production of Biomass-Degrading Enzymes by Trichoderma reesei Using Liquid Hot Water-Pretreated Corncob in Different Conditions of Oxygen Transfer

“…Additionally, this pretreatment presents some advantages compared to other pretreatment technologies, like simple and economical operation, limited equipment corrosion problems, reduced polysaccharide losses, and inhibitor formation; does not require the addition and recovery of chemicals different from water; and is considered an environmentally friendly process [12]. Many researchers have used lignocellulosic hydrolysates and pretreated solids for enzyme production and have showed that these pretreated materials improve enzyme production [11][12][13][14][15][16].…”