Media optimization for β-Fructofuranosidase production by Aspergillus oryzae

Abstract

-β-Fructofuranosidase production by Aspergillus oryzae IPT301 was maximized in shake flasks. Response Surface Methodology (RSM) involving Small Central Composite Design was adopted to evaluate the fructosyltransferase (FTase) activity by changing three medium component concentrations: sucrose, urea and yeast extract. The optimal set of conditions for maximum fructosyltransferase production was as follows: sucrose 320.5 g/L, urea 7.13 g/L and yeast extract 2.11 g/L. In this optimal condition, the following improvements were achieved: an increase of 48.8% in cell growth, 112% and 62% in micelial and free FTase activities, respectively, 62.8% in the ratio of fructosyltransferase/hydrolytic activities for enzyme linked to mycelium and 67.5% for free enzyme.

Production and biochemical characterization of an alkaline protease from Aspergillus oryzae CH93

“…3d). Urea was the best 303 nitrogen source for growth A. oryzae IPT-301 and enzyme production as reported by 304 Ottoni et al (20); whereas, Devi showed ammonium sulfate was the best nitrogen 305 sources for Aspergillus niger (28). Our results showed that yeast extract was the 306 most suitable nitrogen source for fungal biomass and enzyme production.…”

“…In each medium, amount of 0.5 McFarland of the strain was 128 added and the amount of enzyme production through enzyme activity growth was 129 measured at 120 RPM and 28 °C after 120 h incubation. To evaluate the influence of 130 7 temperature, in three different liquid medium amount of 0.5 McFarland of the strain 131 was added and the inoculated medium was incubated at 17°C, 28°C and 37°C.132 To determine effects of different carbon and nitrogen sources on protease133 production, in six different liquid mediums, amount 1% (w/v) of glucose, maltose, 134 fructose, yeast extract, urea and ammonium sulfate was added(20).Following 135 addition 0.5 McFarland of the strain and incubation under optimal standard 136 conditions, the enzyme activity was measured over five days. activity was conducted based on Moradian et al with slight modification 140 (23).…”

Influence of pectin, glucose, and pH on the production of endo- and exo-polygalacturonase by Aspergillus oryzae in liquid medium

“…In previous works (Fontana et al, 2009;Ottoni et al, 2012), important effects of simple carbohydrates on the growth and enzyme production by A. oryzae IPT 301 were observed. As such, the effect of glucose concentration on the batch process was tested in WBE medium containing initial glucose concentrations up to 50 g.L -1 in runs without pH control (G0 to G30) or with pH controlled to a minimum value of 2.7 (G20 * to G50 * ).…”

Statistical optimization of medium compositions for chitosanase production by a newly isolated Streptomyces albus

“…Once the ranges of the relevant variables were selected through the PB screening and steepest ascent experiment, Box-Behnken design (BBD) and response surface methodology (RSM) were used to obtain a quadratic model, and then appraise the quadratic results and central points to estimate the pure process variability , Ottoni et al, 2012, with chitosanase activity as response. Three compositions, i.e., glucose, peptone and MgSO 4 , that significantly affected chitosanase activity were optimized by RSM using a 3-factor-3-level BBD.…”