The effect of dissolved oxygen concentration control on cell growth and antibiotic retamycin production in Streptomyces olindensis So20 fermentations

Abstract

-Cell growth and retamycin production in Streptomyces olindensis So20 were studied at different dissolved oxygen levels during fermentation. The profiles for cell growth and retamycin production in an experiment with dissolved oxygen control at 5% air saturation during production were similar to those in an experiment without dissolved oxygen control. However, despite slower cell growth, a twofold increase in the final retamycin concentration was achieved when dissolved oxygen was controlled at 100% during the growth phase, compared to a base experiment (no DO control). Therefore, the results revealed that retamycin production was dependent on dissolved oxygen concentration during the growth phase, even with sufficient oxygen. On the other hand, a large oxygen supply during the production phase did not contribute to an improvement in retamycin production.

Anti-Methicillin Resistant Staphylococcus aureus Activity and Optimal Culture Condition of Streptomyces sp. SUK 25

“…The flasks were incubated for 7 days at 28°C with aeration rate of 160rpm. Eight fermentation medium with modified formula were used, namely A 3 M Media ( 11 ), Bn-2 Media ( 11 ), ISP 9 Media ( 12 ), Czapek-Dox Media ( 13 ), Bennette Media ( 6 ), Thronton’s Media ( 14 ), Heydorn Media ( 15 ) and Nutrient Broth (Merck, USA). After that, the selected media was optimized based on its anti-MRSA activities, whereby the involved parameters were incubation period, pH level of the media and aeration rate.…”

Oxygen supply controls the onset of pristinamycins production by Streptomyces pristinaespiralis in shaking flasks

“…Some studies have been specifically conducted to observe the role of dissolved oxygen concentration (DOC) on the concentration of produced antibiotics. For example, a two‐fold increase in the final ratamycin concentration has been observed when DOC was controlled at a value of 100% of air saturation (Martins et al, 2004). A linear relation between the concentration of virginamycin produced by Streptomyces virginiae and the dissolved oxygen tension from 10 to 80% was put into evidence by Shioya et al (1999).…”

Development of Fed-Batch Cultivation Strategy for Efficient Oxytetracycline Production by Streptomyces rimosus at Semi-Industrial Scale

“…However, in addition to the advantages of this approach to prevent carbon source limitation and substrate overflow, this cultivation strategy also helped to control the DO value at certain levels during the cultivation. In most processes for antibiotic production by aerobic microorganisms such as actinomycetes, DO controls the cell growth and product biosynthesis significantly and is considered as secondary metabolite limiting substrate for different types of antibiotics (Collins et al 1989;Martins et al 2004;Wang and Zhang 2007).…”