The influence of oxygen supply on the production of acetaldehyde by Zymomonas mobilis

Mastroeni, Marco Fábio; Gurgel, Patrick V.; Silveira, Maurício Moura da; Mancilha, Ismael Maciel de; Jonas, Rainer

doi:10.1590/s0104-66322003000200001

Cited by 4 publications

(3 citation statements)

References 6 publications

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“…It is possible that some fraction of this missing carbon evaporated as acetaldehyde given that acetaldehyde production by aerobically growing Z. mobilis has been previously reported and that our culturing conditions and sample preparation were not optimized for highly volatile compounds (16, 43).…”

Section: Resultsmentioning

confidence: 99%

Systems-Level Analysis of Oxygen Exposure in Zymomonas mobilis : Implications for Isoprenoid Production

et al. 2019

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Microbially generated biofuels and bioproducts have the potential to provide a more environmentally sustainable alternative to fossil-fuel-derived products. In particular, isoprenoids, a diverse class of natural products, are chemically suitable for use as high-grade transport fuels and other commodity molecules. However, metabolic engineering for increased production of isoprenoids and other bioproducts is limited by an incomplete understanding of factors that control flux through biosynthetic pathways. Here, we examined the native regulation of the isoprenoid biosynthetic pathway in the biofuel producer Zymomonas mobilis. We leveraged oxygen exposure as a means to perturb carbon flux, allowing us to observe the formation and resolution of a metabolic bottleneck in the pathway. Our multi-omics analysis of this perturbation enabled us to identify key auxiliary enzymes whose expression correlates with increased production of isoprenoid precursors, which we propose as potential targets for future metabolic engineering.

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Section: Resultsmentioning

confidence: 99%

Systems-Level Analysis of Oxygen Exposure in Zymomonas mobilis : Implications for Isoprenoid Production

et al. 2019

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“…where k L a is the mass transfer coefficient for oxygen which is positively correlated with the impeller speed during fermentation ( Mastroeni et al, 2003 ) and assumed to be 30 h –1 at 300 rpm. [O 2 ∗ ] denotes the oxygen concentration in gas phase and assumed to be a constant 0.21 mM ( Mahadevan et al, 2002 ).…”

Section: Methodsmentioning

confidence: 99%

“…where k L a is the mass transfer coefficient for oxygen which is positively correlated with the impeller speed during fermentation (Mastroeni et al, 2003) and assumed to be 30 h −1 at 300 rpm.…”

Section: Dynamic Flux Balance Modelmentioning

confidence: 99%

Thermodynamic and Kinetic Modeling of Co-utilization of Glucose and Xylose for 2,3-BDO Production by Zymomonas mobilis

Spiller

Dowe

et al. 2021

Front. Bioeng. Biotechnol.

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Prior engineering of the ethanologen Zymomonas mobilis has enabled it to metabolize xylose and to produce 2,3-butanediol (2,3-BDO) as a dominant fermentation product. When co-fermenting with xylose, glucose is preferentially utilized, even though xylose metabolism generates ATP more efficiently during 2,3-BDO production on a BDO-mol basis. To gain a deeper understanding of Z. mobilis metabolism, we first estimated the kinetic parameters of the glucose facilitator protein of Z. mobilis by fitting a kinetic uptake model, which shows that the maximum transport capacity of glucose is seven times higher than that of xylose, and glucose is six times more affinitive to the transporter than xylose. With these estimated kinetic parameters, we further compared the thermodynamic driving force and enzyme protein cost of glucose and xylose metabolism. It is found that, although 20% more ATP can be yielded stoichiometrically during xylose utilization, glucose metabolism is thermodynamically more favorable with 6% greater cumulative Gibbs free energy change, more economical with 37% less enzyme cost required at the initial stage and sustains the advantage of the thermodynamic driving force and protein cost through the fermentation process until glucose is exhausted. Glucose-6-phosphate dehydrogenase (g6pdh), glyceraldehyde-3-phosphate dehydrogenase (gapdh) and phosphoglycerate mutase (pgm) are identified as thermodynamic bottlenecks in glucose utilization pathway, as well as two more enzymes of xylose isomerase and ribulose-5-phosphate epimerase in xylose metabolism. Acetolactate synthase is found as potential engineering target for optimized protein cost supporting unit metabolic flux. Pathway analysis was then extended to the core stoichiometric matrix of Z. mobilis metabolism. Growth was simulated by dynamic flux balance analysis and the model was validated showing good agreement with experimental data. Dynamic FBA simulations suggest that a high agitation is preferable to increase 2,3-BDO productivity while a moderate agitation will benefit the 2,3-BDO titer. Taken together, this work provides thermodynamic and kinetic insights of Z. mobilis metabolism on dual substrates, and guidance of bioengineering efforts to increase hydrocarbon fuel production.

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Significance of oxygen supply in production of a novel antibiotic by Pseudomonas sp. SJT25

Zhong

2011

Bioresource Technology

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The influence of oxygen supply on the production of acetaldehyde by Zymomonas mobilis

Cited by 4 publications

References 6 publications

Systems-Level Analysis of Oxygen Exposure in Zymomonas mobilis : Implications for Isoprenoid Production

Systems-Level Analysis of Oxygen Exposure in Zymomonas mobilis : Implications for Isoprenoid Production

Thermodynamic and Kinetic Modeling of Co-utilization of Glucose and Xylose for 2,3-BDO Production by Zymomonas mobilis

Significance of oxygen supply in production of a novel antibiotic by Pseudomonas sp. SJT25

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