The influence of substrate source on the growth of Ralstonia eutropha, aiming at the production of polyhydroxyalkanoate

Marangoni, Cintia; Fúrigo, Agenor; Aragão, Gláucia Maria Falcão de

doi:10.1590/s0104-66322001000200005

Cited by 14 publications

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“…6 ). Also, galactose and mannose existing in sunflower stalk hydrolysate solution was completely consumed by recombinant R. eutropha NCIMB11599 (pKM212-XylAB) in 96 h as previous reported [ 45 , 46 ]. When this strain was cultured in MR-N medium containing 1:5 diluted hydrolysate solution of sunflower stalk, it produced 2.42 g/L of P(3HB) with a P(3HB) content of 75.42 wt% by consuming 5.61 g/L of glucose, 0.431 g/L of xylose, 0.00101 g/L of galactose, and 0.0744 g/L of mannose.…”

Section: Resultssupporting

confidence: 73%

Recombinant Ralstonia eutropha engineered to utilize xylose and its use for the production of poly(3-hydroxybutyrate) from sunflower stalk hydrolysate solution

Kim

Jang

et al. 2016

Microb Cell Fact

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BackgroundLignocellulosic raw materials have extensively been examined for the production of bio-based fuels, chemicals, and polymers using microbial platforms. Since xylose is one of the major components of the hydrolyzed lignocelluloses, it is being considered a promising substrate in lignocelluloses based fermentation process. Ralstonia eutropha, one of the most powerful and natural producers of polyhydroxyalkanoates (PHAs), has extensively been examined for the production of bio-based chemicals, fuels, and polymers. However, to the best of our knowledge, lignocellulosic feedstock has not been employed for R. eutropha probably due to its narrow spectrum of substrate utilization. Thus, R. eutropha engineered to utilize xylose should be useful in the development of microbial process for bio-based products from lignocellulosic feedstock.ResultsRecombinant R. eutropha NCIMB11599 expressing the E. coli xylAB genes encoding xylose isomerase and xylulokinase respectively, was constructed and examined for the synthesis of poly(3-hydroxybutyrate) [P(3HB)] using xylose as a sole carbon source. It could produce 2.31 g/L of P(3HB) with a P(3HB) content of 30.95 wt% when it was cultured in a nitrogen limited chemically defined medium containing 20.18 g/L of xylose in a batch fermentation. Also, recombinant R. eutropha NCIMB11599 expressing the E. coli xylAB genes produced 5.71 g/L of P(3HB) with a P(3HB) content of 78.11 wt% from a mixture of 10.05 g/L of glucose and 10.91 g/L of xylose in the same culture condition. The P(3HB) concentration and content could be increased to 8.79 g/L and 88.69 wt%, respectively, when it was cultured in the medium containing 16.74 g/L of glucose and 6.15 g/L of xylose. Further examination of recombinant R. eutropha NCIMB11599 expressing the E. coli xylAB genes by fed-batch fermentation resulted in the production of 33.70 g/L of P(3HB) in 108 h with a P(3HB) content of 79.02 wt%. The concentration of xylose could be maintained as high as 6 g/L, which is similar to the initial concentration of xylose during the fed-batch fermentation suggesting that xylose consumption is not inhibited during fermentation. Finally, recombinant R. eutorpha NCIMB11599 expressing the E. coli xylAB gene was examined for the production of P(3HB) from the hydrolysate solution of sunflower stalk. The hydrolysate solution of sunflower stalk was prepared as a model lignocellulosic biomass, which contains 78.8 g/L of glucose, 26.9 g/L of xylose, and small amount of 4.8 g/L of galactose and mannose. When recombinant R. eutropha NCIMB11599 expressing the E. coli xylAB genes was cultured in a nitrogen limited chemically defined medium containing 23.1 g/L of hydrolysate solution of sunflower stalk, which corresponds to 16.8 g/L of glucose and 5.9 g/L of xylose, it completely consumed glucose and xylose in the sunflower stalk based medium resulting in the production of 7.86 g/L of P(3HB) with a P(3HB) content of 72.53 wt%.ConclusionsRalstonia eutropha was successfully engineered to utilize xylose as a sole carbon source as w...

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Section: Resultssupporting

confidence: 73%

Recombinant Ralstonia eutropha engineered to utilize xylose and its use for the production of poly(3-hydroxybutyrate) from sunflower stalk hydrolysate solution

Kim

Jang

et al. 2016

Microb Cell Fact

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“…Researchers have used various strategies to lower the cost of production, which include the use of metabolically engineered strains, various fermentation strategies, efficient downstream processing and transgenic plants. Facts state that the cost of the carbon source utilized in the production of PHAs accounts for 31% of the total production cost (Kim and Rhee, ; Marangoni et al ., , ). Therefore, one of the most efficient approaches in making PHA production a cost effective process would be to utilize cheap carbon sources.…”

Section: Introductionmentioning

confidence: 99%

Production of a novel medium chain length poly(3‐hydroxyalkanoate) using unprocessed biodiesel waste and its evaluation as a tissue engineering scaffold

Basnett

Lukasiewicz

Marcello

et al. 2017

Microbial Biotechnology

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SummaryThis study demonstrated the utilization of unprocessed biodiesel waste as a carbon feedstock for Pseudomonas mendocina CH50, for the production of PHAs. A PHA yield of 39.5% CDM was obtained using 5% (v/v) biodiesel waste substrate. Chemical analysis confirmed that the polymer produced was poly(3‐hydroxyhexanoate‐co‐3‐hydroxyoctanoate‐co‐3‐hydroxydecanoate‐co‐3‐hydroxydodecanoate) or P(3HHx‐3HO‐3HD‐3HDD). P(3HHx‐3HO‐3HD‐3HDD) was further characterized and evaluated for its use as a tissue engineering scaffold (TES). This study demonstrated that P(3HHx‐3HO‐3HD‐3HDD) was biocompatible with the C2C12 (myoblast) cell line. In fact, the % cell proliferation of C2C12 on the P(3HHx‐3HO‐3HD‐3HDD) scaffold was 72% higher than the standard tissue culture plastic confirming that this novel PHA was indeed a promising new material for soft tissue engineering.

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“…Na busca por matérias-primas alternativas de baixo custo para a produção de PHB, Marangoni e colaboradores 40 estudaram R. eutropha DSM 545 em frascos agitados, onde foram testados lactose hidrolisada, açúcar invertido e água de maceração de milho sob diferentes temperaturas (30 e 34 ºC), sugerindo-se a água de maceração de milho como alternativa viável como fonte de nitrogênio.…”

Section: Produção De Phb Por Ralstonia Eutrophaunclassified

Produção biotecnológica de poli-hidroxialcanoatos para a geração de polímeros biodegradáveis no Brasil

et al. 2007

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The influence of substrate source on the growth of Ralstonia eutropha, aiming at the production of polyhydroxyalkanoate

Cited by 14 publications

References 0 publications

Recombinant Ralstonia eutropha engineered to utilize xylose and its use for the production of poly(3-hydroxybutyrate) from sunflower stalk hydrolysate solution

Recombinant Ralstonia eutropha engineered to utilize xylose and its use for the production of poly(3-hydroxybutyrate) from sunflower stalk hydrolysate solution

Production of a novel medium chain length poly(3‐hydroxyalkanoate) using unprocessed biodiesel waste and its evaluation as a tissue engineering scaffold

Produção biotecnológica de poli-hidroxialcanoatos para a geração de polímeros biodegradáveis no Brasil

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