Dynamic Modelling and Advanced Predictive Control of a Continuous Process of Enzyme Purification

Abstract

A dynamic mathematical model, simulation and computer control of a Continuous Affinity Recycle Extraction (CARE) process, a protein purification technique based on protein adsorption on solid-phase adsorbents is described in this work. This process, consisting of three reactors, is a multivariable process with considerable time delay in the on-line analyses of the controlled variable. An advanced predictive control configuration, specifically the Dynamic Matrix Control (DMC), was applied. The DMC algorithm was applied in process schemes where the aim was to maintain constant the enzyme concentration in the outlet of the third reactor. The performance of the DMC controller was analyzed in the feed-flow disturbances and the results are presented

Enzyme Production

“…Continuous affinity recycle extraction is based on the combination of affinity chromatography with molecular filtration. The system is simple, scalable and useful as a front or final purification step and has been used for the purification of β-galactosidase (Pungor et al 1987) and the process modelled and optimized (Gordon et al 1990;Sun et al 1995;Dechechi et al 1997).…”

Modelagem e desenvolvimento de estrategia de controle para o reator de sintese de dextrana

“…O sistema CARE proposto originalmente consistia de um sistema contínuo duplo estágio: um de adsorção e outro de dessorção. Já foram sugeridas modificações no projeto original, como o acréscimo de um estágio para retirada de contaminantes e maior purificação da enzima (DECHECHI et al, 1997). SOUZA (1993) • peso molecular alto (superior a 25 milhões de daltons)-como componente da lama de perfuração e também na recuperação secundária de petróleo.…”