Molecular analysis of rubella virus in travelers suspected of measles infection in São Paulo, Brazil

Figueiredo, Cristina; Yu, Ana Lúcia Frugis; Afonso, Ana Maria Sardinha; Curti, Suely Pires; Oliveira, Maria Isabel de

doi:10.1590/s0104-42302012000500007

Cited by 3 publications

(4 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the B3.3 lineage previously reported to be the dominant circulating sub-genotype in the period 2009–2011 in Senegal [ 17 ] was not detected in the present study, possibly due to a non-exhaustive sequencing. The single Rubella sequence obtained in this study belongs to the genotype 1C, a genotype which circulated in Brazil, Peru and the West Coast of South America [ 48 ]. Further molecular analysis of rubella viruses in Senegal will be challenged by the ongoing progress towards elimination, although it may play an important role in the containment and surveillance of future outbreaks.…”

Section: Discussionmentioning

confidence: 99%

Measles and Rubella Incidence and Molecular Epidemiology in Senegal: Temporal and Regional Trends during Twelve Years of National Surveillance, 2010–2021

Jallow

Sadio

Mendy

et al. 2022

Viruses

View full text Add to dashboard Cite

We investigated the epidemiology of measles and rubella infections in Senegal based on data from twelve consecutive years of laboratory-based surveillance (2010–2021) and conducted phylogenetic analyses of circulating measles viruses. Sera from measles-suspected cases were collected and tested for measles and rubella-specific IgM antibodies using enzyme-linked immunosorbent assays (ELISA). Throat swabs were collected from patients with clinically diagnosed measles for confirmation by reverse-transcription polymerase chain reaction (RT-PCR) and viral genotyping. Among 8082 laboratory-tested specimens from measles-suspected cases, serological evidence of measles and rubella infection was confirmed in 1303/8082 (16.1%) and 465/6714 (6.9%), respectively. The incidence of rubella is now low—0.8 (95% CI 0.4–1.3) cases per million people in 2021—whereas progress towards measles pre-elimination targets (<1.0 case per million people per year) appears to have stalled; there were 10.8 (95% CI 9.3–12.5) cases per million people in 2021. Phylogenetic analyses revealed that all Senegalese measles strains belonged to genotype B3. The rubella virus sequence obtained in this study was consistent with genotype 1C. Our national surveillance data suggest that despite their low incidence both measles and rubella remain endemic in Senegal with a concerning stagnation in the decline of measles infections that represents a significant challenge to the goal of regional elimination.

show abstract

Section: Discussionmentioning

confidence: 99%

Measles and Rubella Incidence and Molecular Epidemiology in Senegal: Temporal and Regional Trends during Twelve Years of National Surveillance, 2010–2021

Jallow

Sadio

Mendy

et al. 2022

Viruses

View full text Add to dashboard Cite

show abstract

“…In Brazil, all cases of fever and rash meeting clinical case definitions for measles, defined as fever and maculopapular rash accompanied by ≥1 of the 3 following symptoms: cough, coryza, or conjunctivitis, in any individual, regardless of age or vaccination status or rubella, defined as fever and maculopapular rash accompanied by retroauricular, occipital, or cervical lymphadenopathy in any individual, regardless of age and vaccination status, should be notified and confirmed or discarded by laboratory testing 11 , 12 . In accordance with the established procedures of the regional measles/rubella laboratory network, composed of Public Health laboratories throughout the State of Sao Paulo, laboratory evaluation of suspected cases and contacts include serologic testing, viral culture, detection of viral RNA by Real Time PCR and viral genotyping 1 , 3 , 7 . For this reason, the present patient was at first misdiagnosed with rubella and only later the correct diagnosis was made.…”

Section: Discussionmentioning

confidence: 99%

“…Viral RNA extraction was performed from urine samples and infected culture cells were processed using PureLink Viral RNA/DNA Mini Kit (Invitrogen, USA) according to the manufacturer’s recommendations. Initially, the Real Time PCR (RT-qPCR) was performed using Measles virus (MV) and Rubella virus (RV) primers, as previously described 1 , 7 . The urine sample and infected culture cells were negative for MV and RV.…”

Section: Case Reportmentioning

confidence: 99%

“…Rubella virus infection is characterized by a maculopapular rash and cervical, suboccipital or postauricular adenopathy or arthralgia/arthriti s 1 . Up to 50% of infections are asymptomatic or subclinical and symptoms are similar to those caused by measles virus and other etiologies associated with rash, so that the clinical diagnosis of rubella is unreliable 2 , 3 .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Isolation of infectious Zika virus from a urine sample cultured in SIRC cells from a patient suspected of having rubella virus

Oliveira

Namiyama

Cabral

et al. 2018

Rev. Inst. Med. trop. S. Paulo

View full text Add to dashboard Cite

A great variety of viruses which cause exanthema share other clinical manifestations, making the etiologic identification a very difficult task, relying exclusively on the clinical examination. Rubella virus (RV) infection during the early stages of pregnancy can lead to serious birth defects, known as congenital rubella syndrome (CRS). In the present report, we described the presence of Zika virus (ZIKV) particles in urine samples and also ZIKV isolation in SIRC cells from the urine of a patient in acute phase of suspected rubella disease. The 50-year-old unvaccinated woman living in Sao Paulo, Brazil, was admitted to the emergency room with fever, headache, rash, arthralgia and prostration. Urine samples were collected for virus isolation and RT-qPCR. SIRC and Vero cells were inoculated with urine samples during 7 days. RT-qPCR was performed using measles virus (MV) and RV primers and both were found to be negative. After this result, RT-qPCR was performed for parvovirus B19, herpes virus 6 and ZIKV. The urine sample and the isolate were positive by Real Time PCR for ZIKV and negative for all other viruses tested. The sequences isolated are from the Asiatic lineage.

show abstract

Seroprevelence of rubella among children suspected of having measles in Gadarif Hospital, Eastern Sudan

et al. 2019

View full text Add to dashboard Cite

While generally mild in children, rubella infection during pregnancy can lead to miscarriage, foetal death or congenital rubella syndrome. Rubella vaccination is not yet available as a part of routine immunisation in Sudan, and the burden of infection is unknown. Using the clinical case definition adopted by the World Health Organisation, a total of 123 children suspected of having of measles were enrolled in this study during January-December 2017 in Gadarif Hospital, Eastern Sudan. Those cases whose sera were negative for measles IgM antibodies were tested for rubella IgM antibody. A confirmed rubella case was a person who tested positive for rubella IgM. Sera were analysed for IgM specific antibodies against measles virus and rubella virus using enzyme-linked immunosorbent assay. Among the total 123 children suspected to have measles, 92 (74.8%) children were positive for measles IgM antibodies. Of the 31 children who had measles IgM antibodies negative, 20 (16.3%) children were seropositive for rubella IgM antibodies. Implementation of a surveillance system will provide the improved estimates of rubella virus and estimated the burden of congenital rubella syndrome. Such information is necessary and it is an important step for future policy decisions for vaccine delivery strategies.

show abstract

Molecular analysis of rubella virus in travelers suspected of measles infection in São Paulo, Brazil

Abstract: Information about RV and MV genotypes circulating in São Paulo is essential for the control of measles, rubella, and congenital rubella syndrome (CRS) in Brazil.

Cited by 3 publications

References 7 publications

Measles and Rubella Incidence and Molecular Epidemiology in Senegal: Temporal and Regional Trends during Twelve Years of National Surveillance, 2010–2021

Measles and Rubella Incidence and Molecular Epidemiology in Senegal: Temporal and Regional Trends during Twelve Years of National Surveillance, 2010–2021

Isolation of infectious Zika virus from a urine sample cultured in SIRC cells from a patient suspected of having rubella virus

Seroprevelence of rubella among children suspected of having measles in Gadarif Hospital, Eastern Sudan

Contact Info

Product

Resources

About