Prevalência dos marcadores imunológicos Anti-GAD e Anti-IA2 em parentes de primeiro grau de diabéticos do tipo 1 em amostra da população da Grande São Paulo

Cesarini, Paulo Roberto; Mendonca, Edrea; Fernandes, Vania; Silva, Regina do Carmo; Morimitsu, Lílian K.; García, F; Vechiatti, Stella; Miranda, Walkíria L.; Dib, Sérgio Atala

doi:10.1590/s0104-42302003000400030

Cited by 10 publications

(7 citation statements)

References 24 publications

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“…It is known that GADA tend to be less frequent in Black African patients with T1D (23). On the other hand, previous Brazilian studies have found a similar prevalence of GADA positivity in Brazilian and Caucasian European T1D (5,24). Finally, we believe that the lack of patients with a very recent diagnosis (<12 months) could be a major factor explaining the low prevalence of GADA in our study population, especially in the group with a shorter duration of the disease.…”

Section: Discussioncontrasting

confidence: 40%

Relationship between the prevalence of anti-glutamic acid decarboxylase autoantibodies and duration of type 1 diabetes mellitus in Brazilian patients

Rodacki¹,

Zajdenverg

Albernaz

et al. 2004

Braz J Med Biol Res

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The objective of the present study was to determine whether the duration of disease has any influence on the prevalence of glutamic acid decarboxylase autoantibodies (GADA) in Brazilian patients with type 1 diabetes (T1D) and variable disease duration. We evaluated 83 patients with T1D. All participants were interviewed and blood was obtained for GADA measurement by a commercial radioimmunoassay (RSR Limited, Cardiff, UK). Four groups of patients were established according to disease duration: A) 1-5 years of disease (N = 24), B) 6-10 years of disease (N = 19), C) 11-15 years of disease (N = 25), and D) >15 years of disease (N = 15). GADA prevalence and its titers were determined in each group. GADA was positive in 38 patients (45.8%) and its frequency did not differ between the groups. The prevalence was 11/24 (45.8%), 8/19 (42.1%), 13/25 (52%), and 6/15 (40%) in groups A, B, C, and D, respectively (P = 0.874). Mean GADA titer was 12.54 ± 11.33 U/ml for the sample as a whole and 11. 95 ± 11.8, 12.85 ± 12.07, 10.57 ± 8.35, and 17.45 ± 16.1 U/ml for groups A, B, C, and D, respectively (P = 0.686). Sex, age at diagnosis or ethnic background had no significant effect on GADA (+) frequency. In conclusion, in this transversal study, duration of disease did not affect significantly the prevalence of GADA or its titers in patients with T1D after one year of diagnosis. This was the first study to report this finding in the Brazilian population.

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Section: Discussioncontrasting

confidence: 40%

Relationship between the prevalence of anti-glutamic acid decarboxylase autoantibodies and duration of type 1 diabetes mellitus in Brazilian patients

Rodacki¹,

Zajdenverg

Albernaz

et al. 2004

Braz J Med Biol Res

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“…We must highlight, however, that all of these positive results were of a low titer (below 10 U/L), but still well above the analytical and functional sensitivities of the technique (0.18 and 4 U/L, respectively) 19. In the control group of a study of beta-cell autoimmunity among children with thyroiditis, the prevalence of GADA was 3.8%,20 while in first-degree relatives of patients with DM1 the prevalence has been 10.1% 21. Higher prevalence of GADA in nondiabetic adults (40.4%) have only been reported among workers exposed to polychlorinated biphenyl products 22.…”

Section: Discussionmentioning

confidence: 98%

Antibodies against glutamic acid decarboxylase and indices of insulin resistance and insulin secretion in nondiabetic adults: a cross-sectional study

Mendivil

Toloza

Ricardo-Silgado

et al. 2017

DMSO

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BackgroundAutoimmunity against insulin-producing beta cells from pancreatic islets is a common phenomenon in type 1 diabetes and latent autoimmune diabetes in adults. Some reports have also related beta-cell autoimmunity to insulin resistance (IR) in type 2 diabetes. However, the extent to which autoimmunity against components of beta cells is present and relates to IR and insulin secretion in nondiabetic adults is uncertain.AimTo explore the association between antibodies against glutamic acid decarboxylase (GADA), a major antigen from beta cells, and indices of whole-body IR and beta-cell capacity/insulin secretion in adults who do not have diabetes.MethodsWe studied 81 adults of both sexes aged 30–70, without known diabetes or any autoimmune disease. Participants underwent an oral glucose tolerance test (OGTT) with determination of plasma glucose and insulin at 0, 30, 60, 90, and 120 minutes. From these results we calculated indices of insulin resistance (homeostasis model assessment of insulin resistance [HOMA-IR] and incremental area under the insulin curve [iAUCins]) and insulin secretion (corrected insulin response at 30 minutes and HOMA beta-cell%). GADAs were measured in fasting plasma using immunoenzymatic methods.ResultsWe found an overall prevalence of GADA positivity of 21.3%, without differences by sex and no correlation with age. GADA titers did not change monotonically across quartiles of any of the IR or insulin secretion indices studies. GADA did not correlate linearly with fasting IR expressed as HOMA-IR (Spearman’s r=−0.18, p=0.10) or postabsorptive IR expressed as iAUCins (r=−0.15, p=0.18), but did show a trend toward a negative correlation with insulin secretory capacity expressed by the HOMA-beta cell% index (r=−0.20, p=0.07). Hemoglobin A1c, body mass index, and waist circumference were not associated with GADA titers.ConclusionGADA positivity is frequent and likely related to impaired beta-cell function among adults without known diabetes.

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“…TPOA were measured by an immunofluorometric assay (autoDelfia, Turku, Finland) and we considered the reference value <52 U/mL for the population studied. Glutamic acid decarboxylase 65 (GADA) and Insulinoma antigen-2 (IA2A) antibodies were measured by a radioimmunoassay (RSR Ltd, Cardiff, UK) and according to previous study of our group we considered the reference value <1.72 and 0.97 U/mL respectively [ 28 ]. Fasting serum C-peptide (FCP) was measured using an immunofluorometric assay (autoDelfia, Turku, Finland) with detection limit of 0.015 ng/mL.…”

Section: Methodsmentioning

confidence: 99%

High frequency of vitamin D receptor gene polymorphism FokI in Brazilian Type 1 diabetes mellitus patients with clinical autoimmune thyroid disease

Mory

Gabbay

Rocco

et al. 2016

Diabetol Metab Syndr

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BackgroundPolymorphisms of vitamin D receptor (VDR) gene have been studied as genetic markers of type 1 diabetes mellitus (T1DM) and some studies have reported associations with autoimmune thyroid disease. The aim of this study was to evaluate the relationship between VDR FokI polymorphism (rs10735810), thyroid autoimmunity and thyroid dysfunction (TD) in Brazilian T1DM.MethodsOne-hundred-eighty T1DM patients were evaluated for age, duration of diabetes (DDM), positivity to TPO Antibody (TPOA), GAD Antibody (GADA), IA2 Antibody (IA2A) and fasting serum C-peptide (FCP) according to diagnosis of TD. PCR–RFLP analyses were carried out for VDR polymorphism FokI.ResultsTPOA positivity (80.0 vs. 25.0 %, p < 0.001) and GADA positivity (56.0 vs. 30.3 %, p = 0.01) were higher in T1DM patients with TD with the same age and DDM than the group without TD, with no difference of FCP and IA2A positivity. We observed higher prevalence of VDR FokI in T1DM with TD (ff and Ff 73.9 % with TD vs. 52.7 % without TD, p = 0.05). Positivity to TPOA and presence of FokI polymorphism were significantly associated with the concurrence of TD in T1DM patients (OR 18.1; CI 3.7–87.0; p < 0.001).ConclusionsThe VDR FokI polymorphism (rs10735810) was associated to persistence of GADA, TPOA positivity and TD in Brazilian T1DM. Positivity to TPOA and VDR polymorphism FokI were strongly associated with concurrence of T1D and TD. These data collaborate to understanding the joint susceptibility genes for TD in T1DM.

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Prevalência dos marcadores imunológicos Anti-GAD e Anti-IA2 em parentes de primeiro grau de diabéticos do tipo 1 em amostra da população da Grande São Paulo

Abstract: The prevalence of anti-GAD has been more prevalent among the PDMI. No differences were observed between prevalences for anti-IA2 showed by PDMI and controls.

Cited by 10 publications

References 24 publications

Relationship between the prevalence of anti-glutamic acid decarboxylase autoantibodies and duration of type 1 diabetes mellitus in Brazilian patients

Relationship between the prevalence of anti-glutamic acid decarboxylase autoantibodies and duration of type 1 diabetes mellitus in Brazilian patients

Antibodies against glutamic acid decarboxylase and indices of insulin resistance and insulin secretion in nondiabetic adults: a cross-sectional study

High frequency of vitamin D receptor gene polymorphism FokI in Brazilian Type 1 diabetes mellitus patients with clinical autoimmune thyroid disease

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