Polímeros usados como sistemas de transporte de princípios ativos

Severino, Patrícia; Santana, Maria Helena Andrade; Malmonge, Sônia Maria; Souto, Eliana B.

doi:10.1590/s0104-14282011005000061

Cited by 19 publications

(15 citation statements)

References 99 publications

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“…Polymeric devices have been successfully used to deliver a wide variety of drugs, including enzymes, hormones, peptides, antibiotics, anti-cancer agents, antifungals, antiinflammatories and analgesics [22][23][24] . In an ideal drug delivery system, the average particle should be <250 µm in diameter in order to avoid the clogging of syringe needles.…”

Section: Morphological Analysis and Average Size Of Microspheresmentioning

confidence: 99%

Preparation and characterization of paclitaxel-loaded PLDLA microspheres

Martins

Messias

Leite³

et al. 2014

Mat. Res.

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Paclitaxel (Taxol®), is a drug used to treat ovarian, breast, lung and bladder cancer. However, the low solubility of this drug in water is a major limitation in its clinical use. One strategy to overcome this limitation would be to encapsulate paclitaxel in polymeric microspheres that are biocompatible and can be used as drug carriers. The aim of this study was to use the bioresorbable, biocompatible copolymer poly-L-co-D,L-lactic acid (PLDLA) in the 70:30 rate to produce and characterize microspheres containing paclitaxel. The simple emulsion technique was used to obtain spherical microspheres that were studied by scanning electron microscopy (SEM) and atomic force microscopy (AFM). The average size of PLDLA microspheres without and with paclitaxel was 10.3 ± 1.7 µm and 12.7 ± 1.3 µm, respectively, as determined by laser light scattering (LLS). Differential scanning calorimetry (DSC) showed that pure paclitaxel had an endothermic peak corresponding to a melting point of 220 °C, which indicated its crystalline nature. The same peak was observed in a physical mixture of PLDLA + paclitaxel in which both components were present in the same proportions used to prepare the microspheres . In contrast, this peak was not observed for the drug, indicating that paclitaxel did not crystallize in PLDLA microspheres. Differential scanning calorimetry (DSC) indicated that paclitaxel was homogeneously dispersed in the PLDLA microspheres, the incorporation of paclitaxel into the microspheres did not alter the thermal properties of PLDLA. The Fourier transform infrared spectroscopy (FTIR) analysis seems to indicate the absence of chemical interaction between polymer and drugs in microspheres and the presence of drugs as a molecular dispersion in the polymer matrix. The efficiency of paclitaxel encapsulation in PLDLA microspheres was 98.0 ± 0.3%, as assessed by high performance liquid chromatography (HPLC). A kinetic study of drug release in vitro using HPLC showed an initial burst release followed by a slower release characteristic of large diameter distribution systems. PLDLA microspheres released 90 ± 4% of the drug over a 30-day period. These findings indicate that PLDLA microspheres are promising carriers for paclitaxel, with a potential for future applications in drug delivery systems.

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Section: Morphological Analysis and Average Size Of Microspheresmentioning

confidence: 99%

Preparation and characterization of paclitaxel-loaded PLDLA microspheres

Martins

Messias

Leite³

et al. 2014

Mat. Res.

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“…Some in vitro methods are used to evaluate the toxicity of medical devices standardized by using cell cultures [37][38][39][40]. Methods involving cell cultures can be successfully employed because they are reproducible, fast, sensitive and easy to execute during an in vitro biocompatibility evaluation [41].…”

Section: Methods For In Vitro Cytotoxicity Analysesmentioning

confidence: 99%

Cytotoxicity Tests for Evaluating Medical Devices: An Alert for the Development of Biotechnology Health Products

Vidal¹,

Granjeiro²

2017

JBiSE

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The risks and damages related to the use of products, technologies and services of sanitary interest can be due to defects or manufacturing failures. Certain products already contain a certain degree of risk, which requires strict quality control in their production, distribution and use, as well as in the disposal of their waste in the environment. With continuous development in science and technology, medical devices must undergo intradermal irritation and testing for sensitization, cytotoxicity, and acute systemic toxicity. In health care, biotechnology aims to provide technology-based products or processes related to energy, food, and health, which are capable of stimulating new businesses, expanding exports, integrating the value chain and stimulating new demands for innovative products and processes, taking into account health policies. The present article was prepared by a bibliographical survey of the electronic databases PubMed, Lilacs, and Bireme. Cell culture testing can be successfully employed, as it is reproducible, rapid, sensitive, and financially accessible for performing in vitro toxicity testing. Thus, it has been possible to optimize the development phase of new products by decreasing animal use or even replacing them in certain tests. Some in vitro assays validated by the Organization for Economic Cooperation and Development in the area of health products have already replaced animal testing.

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“…(SEVERINO et al, 2011) A figura 20 representa os cromatogramas das medidas realizadas para o PLA, obtidos no intervalo da área de permeação seletiva (área de exclusão por tamanho) e a curva de calibração (ajustada ao polinômio de terceiro grau) para os padrões de poliestireno (PS). O erro da medida entre a duplicata foi de 0,13% para a ̅̅̅̅ e 5 % para ̅̅̅̅̅ e são relacionados a precisão do cromatógrafo.…”

Section: Determinação Da Massa Molar Da Matriz De Pla Por Gpcunclassified

“…Nestes casos, para haver liberação dos ativos é necessário que a matriz sofra algum tipo de erosão ou outra ação. (SEVERINO et al, 2011) Segundo (WICHERT; ROHDEWALD, 1990) a degradação de sistemas de micropartículas de PLA é relatado como dependente da massa molar do polímero e varia de semanas a meses de estabilidade.…”

Section: Determinação Da Massa Molar Da Matriz De Pla Por Gpcunclassified

“…( SEVERINO et al, 2011) Com estes dados comprova-se que a solução de nanopartículas poliméricas com ativo é um sistema para liberação controlada simples e de fácil obtenção. Comparando as duas amostras, as larguras diferentes dos picos de difração implicam em tamanho de cristalitos diferentes, o que pode indicar que as moléculas do PLA tenham sofrido algum tipo de orientação influenciando o deslocamento e a assimetria de pico.…”

Section: Estudo De Liberação Do Ativounclassified

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Estudo de formação e estabilidade de nanopartículas de poliácido lático para liberação controlada do óleo essencial de Shinus Molle L.

Silva¹

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Polímeros usados como sistemas de transporte de princípios ativos

Cited by 19 publications

References 99 publications

Preparation and characterization of paclitaxel-loaded PLDLA microspheres

Preparation and characterization of paclitaxel-loaded PLDLA microspheres

Cytotoxicity Tests for Evaluating Medical Devices: An Alert for the Development of Biotechnology Health Products

Estudo de formação e estabilidade de nanopartículas de poliácido lático para liberação controlada do óleo essencial de Shinus Molle L.

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