Preparation and antibacterial activity of silver nanoparticles impregnated in bacterial cellulose

Abstract

Polymer nanocomposites containing metal nanoparticles can be prepared by different methods: mechanical mixing of a polymer with metal nanoparticles; in situ polymerization of a monomer in the presence of metal nanoparticles; or in situ reduction of metal salts or complexes in a polymer [8,9] . These polymer nanocomposites have attracted a great deal of attention due to their unique properties and applications [10,11] . The literature describes many methods to prepare ultrafine silver powders [12][13][14][15] including the formation of Ag nanoparticles attached to bacterial cellulose [2,3] . In this work, we developed an easy method to produce composites with homogeneous size distribution of silver nanoparticles. This structure provides a robust, highlyporous and self-sustaining structure with large surface area, which is essential to facilitate incorporation of the silver ions in the metallization process to give a high silver loading content. Furthermore, the in situ direct metallization method was adopted to obtain a high loading content and strong bonding force of silver nanoparticles on the BC surface, thereby avoiding the Ag + contamination problem. The combination of the antibacterial efficacy of the silver nanoparticles and the biodegradability of the BC fibers in the composite fibers can make them practical for use as antimicrobial membranes in medical applications. Experimental MaterialsThe bacterial cellulose (BC) membranes were supplied by Fibrocel -Produtos Biotecnológicos Ltda. (Ibiporã, Brazil). Polyvinylpyrrolidone (PVP, MW = 29,000), gelatin,

Preparation of Bacterial Cellulose/Inorganic Gel of Bentonite Composite by In Situ Modification

“…However, because of its poor dissolution properties and processing difficulties, BC's application as water adsorbent for water conservation is still limited [9,[11][12][13][14][15][16][17]. Usually, BC modification could be done with some physical or chemical methods to enhance its performance [18][19][20][21].…”

Evaluation of cell disruption for partial isolation of intracellular pyruvate decarboxylase enzyme by silver nanoparticles method

“…This might be due to the high reactivity of silver ions, which readily bounded to electron donor groups. The prime targets were thiol groups (-SH), which are commonly found in enzymes within the microbe (MARIA et al, 2010). This caused the enzyme to denature with certain degree of microbial cell disruption at the higher silver nanoparticle concentration level of 15 μg ml -1 .…”

Bacterial Cellulose for Skin Repair Materials

“…A simple method was also developed to load a large amount of silver nanoparticles into BC. These composite fibers showed nearly 100% antibacterial activities against Escherichia coli (Maria et al, 2010). A facile method was developed to prepare a magnetic Ag nanocomposite.…”