Low-temperature sterilization and new technologies

Goveia, Vânia Regina; Pinheiro, Silma Maria Cunha; Graziano, Kazuko Uchikawa

doi:10.1590/s0104-11692007000300002

Cited by 10 publications

(9 citation statements)

References 10 publications

(3 reference statements)

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“…The choice of this micro-organism was justified by the fact that this is the standard biological indicator for monitoring sterilization cycles by means of ethylene oxide (EtO), the method was chosen for the experiments due to its high diffusibility and compatibility with the thermo-sensitive materials (6) . Lopes CLBC, Graziano KU, Pinto TJA.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of single-use reprocessed laparoscopic instrument sterilization

Lopes

Graziano

Pinto

2011

Rev. Latino-Am. Enfermagem

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This experimental, comparative, laboratory study evaluated the effectiveness of the sterilization of single-use laparoscopic instruments -SULIs (grasper, dissector, scissors, Veress needle and electrosurgical probe system), after contamination-challenge with bacterial spores and sheep blood, and compared the results of the sterilization tests with those of the equivalent reusable instruments. The cleaning methods used were; ultrasonic washer with pulsatile water jet and enzymatic detergent, manual cleaning, cleaning with pressurized water and rinsing. The SULIs were sterilized with ethylene oxide and the reusable instruments in an autoclave. Sterility tests showed 100% negative results for recovery of contaminate microorganisms in both groups. It was concluded that, regarding the sterilization, that it is possible to reprocess SULIs. Evaluación de la esterilidad del instrumental laparoscópico descartable reprocesadoSe trata de un estudio, experimental, de laboratorio y comparativo, que evaluó la eficacia de la esterilidad de los instrumentos laparoscópicos de uso único(ILUU): grasper, disector, tijera, aguja de Veres y el sistema de sonda electroquirúrgica, después de "contaminación desafío" con esporas bacterianas y sangre de carnero; los resultados de las pruebas de esterilidad fueron comparados con los de los instrumentos equivalentes "permanentes". Para efectuar la limpieza se utilizó: lavadora ultrasónica con chorro pulsante y detergente enzimático, limpieza manual, agua bajo presión y enjuague.Los ILUU fueron esterilizados con óxido de etileno, los instrumentos "permanentes" en autoclave. Las pruebas de esterilidad mostraron resultados 100% negativos para la recuperación de los microorganismos contaminantes en los dos grupos. Se concluye que, en relación al alcance de la esterilidad, es posible reprocesar los ILUU.

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Section: Methodsmentioning

confidence: 99%

Evaluation of single-use reprocessed laparoscopic instrument sterilization

Lopes

Graziano

Pinto

2011

Rev. Latino-Am. Enfermagem

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“…In general, cellulose, water and any other materials that adsorb or decompose the gas or sterilant will decrease plasma sterilization efficacy . Relatively little is known about the influence of plasma gas sterilization on the physicochemical and mechanical properties of polymeric biomaterials or glass and its impact on biocompatibility …”

Section: Introductionmentioning

confidence: 99%

“…7 Relatively little is known about the influence of plasma gas sterilization on the physicochemical and mechanical properties of polymeric biomaterials or glass and its impact on biocompatibility. 8,9 The feasibility of gamma irradiation for the sterilization of water and pharmaceutical products has been under intensive research. Sterilization with cobalt-60, for instance, requires a relatively high working dose of 30-33 Gy.…”

mentioning

confidence: 99%

Nanoscale topographic changes on sterilized glass surfaces affect cell adhesion and spreading

Wittenburg

Lauer

Oswald

et al. 2013

J Biomedical Materials Res

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Producing sterile glass surfaces is of great importance for a wide range of laboratory and medical applications, including in vitro cell culture and tissue engineering. However, sterilization may change the surface properties of glass and thereby affect its use for medical applications, for instance as a substrate for culturing cells. To investigate potential effects of sterilization on glass surface topography, borosilicate glass coverslips were left untreated or subjected to several common sterilization procedures, including low-temperature plasma gas, gamma irradiation and steam. Imaging by atomic force microscopy demonstrated that the surface of untreated borosilicate coverslips features a complex landscape of microislands ranging from 1000 to 3000 nm in diameter and 1 to 3 nm in height. Steam treatment completely removes these microislands, producing a nanosmooth glass surface. In contrast, plasma treatment partially degrades the microisland structure, while gamma irradiation has no effect on microisland topography. To test for possible effects of the nanotopographic structures on cell adhesion, human gingival fibroblasts were seeded on untreated or sterilized glass surfaces. Analyzing fibroblast adhesion 3, 6, and 24 h after cell seeding revealed significant differences in cell attachment and spreading depending on the sterilization method applied. Furthermore, single-cell force spectroscopy revealed a connection between the nanotopographic landscape of glass and the formation of cellular adhesion forces, indicating that fibroblasts generally adhere weakly to nanosmooth but strongly to nanorough glass surfaces. Nanotopographic changes induced by different sterilization methods may therefore need to be considered when preparing sterile glass surfaces for cell culture or biomedical applications.

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“…These results raise some concern regarding the criteria used for the classification of products as "single use" or "reusable". It seems that the 'single use' label on health products is determined by manufacturers, based mainly on the properties of the material of which they are made (non-noble plastic or some other type of elastomer) (12) .…”

Section: Discussionmentioning

confidence: 99%

The sterilization efficacy of reprocessed single use diathermy pencils

Neto

Graziano

Padoveze

et al. 2010

Rev. Latino-Am. Enfermagem

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In Brazil, single use diathermy pencils (SUDP) are among the most common reused devices.This study assesses the sterilization efficacy of reprocessing SUDP using two cleansing methods (manual or automated), followed by one of three of the low-temperature sterilization methods: Hydrogen Peroxide Plasma (HPP), Ethylene Oxide (ETO) or Low-Temperature Steam Formaldehyde (LTSF). The sample was composed of 360 SUDP after their first use.The probability of sterilization failure was estimated considering the number of positive microbiological results obtained by cultures of the studied devices. The overall sterilization failure probability for SUDP was 0.26. The sterilization method, which presented the lowest failure probability was the LTSF (0.01), followed by ETO (0.21) and HPP (0.56). Automated cleansing obtained a better result than manual cleansing. This trial demonstrated that the probability of sterilization in reprocessed SUDP is highly dependent on both the type of cleansing and the sterilization method applied. La eficacia de la esterilización del bisturí eléctrico tipo lápiz de uso único reprocesadosEn Brasil, el bisturí eléctrico tipo lápiz de uso único (BETLUU) es uno de los artículos más comúnmente reutilizados. El presente estudio evaluó la eficacia de la esterilización de

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Low-temperature sterilization and new technologies

Cited by 10 publications

References 10 publications

Evaluation of single-use reprocessed laparoscopic instrument sterilization

Evaluation of single-use reprocessed laparoscopic instrument sterilization

Nanoscale topographic changes on sterilized glass surfaces affect cell adhesion and spreading

The sterilization efficacy of reprocessed single use diathermy pencils

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