Physiological and symbiotic diversity of Cupriavidus necator strains isolated from nodules of Leguminosae species

Florentino, Ligiane Aparecida; Jaramillo, Paula Marcela Duque; Silva, Karina Barroso; Silva, Jacqueline Savana da; Oliveira, Silvia Maria de; Moreira, Fátima Maria de Souza

doi:10.1590/s0103-90162012000400003

Cited by 16 publications

(4 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Nestes solos, os valores de pH ficaram abaixo de 5,4 (Quadro 1). Estes resultados estão de acordo com os observados por Florentino et al (2012), em que A. doebereinerae somente se desenvolve quando cultivada em meio de cultura contendo valores de pH acima de 6,0.…”

Section: Resultsunclassified

Ocorrência e eficiência do microssimbionte de Sesbania virgata(Cav.) Pers. em função das propriedades do solo

Florentino¹,

Nunes²,

Rezende³

et al. 2015

Rev. Ciênc. Agr.

View full text Add to dashboard Cite

R E S U M OA leguminosa Sesbania virgata apresenta grande importância económica e ecológica, podendo ser utilizada em áreas de recuperação de solos degradados e reflorestamento de matas ciliares. S. virgata estabelece simbiose específica e eficiente com rizóbios da espécie Azorhizobium doebereinerae. O objetivo deste trabalho foi verificar a influência do pH e saturação por bases (V%) na sobrevivência de A. doebereinerae no solo e no estabelecimento da simbiose com S. virgata. O ensaio foi instalado em estufa em vasos contendo 5,5 dm 3 de solo apresentando diferentes valores de V%: 22, 30, 40, 50, 60 e 70%. Observou-se a presença de A. doebereinerae no solo somente nos tratamentos que apresentaram valores de V% superiores a 50. Em relação à simbiose, foi verificado que a inoculação com A. doebereinerae em S. virgata não é eficiente em solos que apresentam valores de pH e V% inferiores a 5,8 e 50, respectivamente. Palavras-chave:Azorhizobium doebereinerae, calagem, especificidade simbiótica, leguminosa. A B S T R A C TThe legume Sesbania virgata has a large economic and ecological importance and can be used in the recovery of areas with degraded soils and reforestation of riparian forests. S. virgata establishes specific and effective symbiosis with rhizobia species Azorhizobium doebereinerae. The objective of this study was to determine the influence of pH and base saturation (V%) on the survival of A. doebereinerae in the soil and in the establishment of the symbiosis with S. virgata. The experiment was conducted in a greenhouse in pots containing 5.5 dm 3 of soil with different values of V%: 22, 30, 40, 50, 60 and 70%. It was observed that A. doebereinerae was only present in treatments that showed values of base saturation greater than 50%. In relation to the symbiosis, it has been found that the inoculation of S. virgata with A. doebereinerae is not effective in soils with pH and V (%) values below 5.8 and 50, respectively. Keywords: Azorhizobium doebereinerae, liming, symbiotic specificity, legume. IntroduçãoUm número considerável de leguminosas arbóreas e arbustivas possui a capacidade de estabelecer simbiose com bactérias que nodulam as leguminosas (BNL), comumente conhecidas por rizóbios. Estudos relacionados com a diversidade de rizóbios presentes em nódulos destas plantas relatam que uma única espécie pode ser hospedeira de rizóbios que apresentam grande diversidade fenotípica e simbiótica, podendo indicar a presença de vários géneros e espécies (Sylla et al., 2002;Rincón-Rosales et al., 2009).A leguminosa Sesbania virgata é nativa do Brasil, apresenta porte arbustivo, crescimento rápido,

show abstract

Section: Resultsunclassified

Ocorrência e eficiência do microssimbionte de Sesbania virgata(Cav.) Pers. em função das propriedades do solo

Florentino¹,

Nunes²,

Rezende³

et al. 2015

Rev. Ciênc. Agr.

View full text Add to dashboard Cite

show abstract

“…After several days, the plates were observed with clear zones around colonies of what turned out to be the bacterial isolate of interest. After proving to be a Gram Negative (GN) species, the Biolog GN2 kit (Biolog, Hayward, CA, USA) was used for initial identification of the isolated bacterium based on substrate utilization patterns 23 . B. subtilis ATCC 49760 was used as a gram positive control.…”

Section: Methodsmentioning

confidence: 99%

Bioremediation of copper in sediments from a constructed wetland ex situ with the novel bacterium Cupriavidus basilensis SRS

Kugler

Brigmon

Friedman

et al. 2022

Sci Rep

View full text Add to dashboard Cite

The H-02 constructed wetland was designed to remove metals (primarily copper and zinc) to treat building process water and storm water runoff from multiple sources associated with the Tritium Facility at the DOE-Savannah River Site, Aiken, SC. The concentration of Cu and Zn in the sediments has increased over the lifetime of the wetland and is a concern. A bioremediation option was investigated at the laboratory scale utilizing a newly isolated bacterium of the copper metabolizing genus Cupriavidus isolated from Tim’s Branch Creek, a second-order stream that eventually serves as a tributary to the Savannah River, contaminated with uranium and other metals including copper, nickel, and mercury. Cupriavidus basilensis SRS is a rod-shaped, gram-negative bacterium which has been shown to have predatory tendencies. The isolate displayed resistance to the antibiotics ofloxacin, tetracycline, ciprofloxacin, select fungi, as well as Cu2+ and Zn2+. Subsequent ribosomal sequencing demonstrated a 100% confidence for placement in the genus Cupriavidus and a 99.014% match to the C. basilensis type strain. When H-02 wetland samples were inoculated with Cupriavidus basilensis SRS samples showed significant (p < 0.05) decrease in Cu2+ concentrations and variability in Zn2+ concentrations. Over the 72-h incubation there were no significant changes in the inoculate densities (106–108 cells/ML) indicating Cupriavidus basilensis SRS resiliency in this environment. This research expands our understanding of the Cupriavidus genus and demonstrates the potential for Cupriavidus basilensis SRS to bioremediate sites impacted with heavy metals, most notably copper.

show abstract

“…In South America, Cupriavidus was uncommon in French Guiana and Uruguay (3-10 % of nodule isolates; [ 9 , 11 ]), and was not detected at all in extensive surveys of Mimosa in central Brazil [ 5 , 6 ]. However, it has been isolated from two cultivated legumes in Minas Gerais, Brazil [ 18 ]. This suggests that further surveys in South America may discover additional wild legume hosts that utilize Cupriavidus symbionts.…”

Section: Introductionmentioning

confidence: 99%

High-quality permanent draft genome sequence of the Mimosa asperata - nodulating Cupriavidus sp. strain AMP6

Meyer¹,

Parker²,

Berkum³

et al. 2015

Stand in Genomic Sci

View full text Add to dashboard Cite

Cupriavidus sp. strain AMP6 is an aerobic, motile, Gram-negative, non-spore-forming rod that was isolated from a root nodule of Mimosa asperata collected in Santa Ana National Wildlife Refuge, Texas, in 2005. Mimosa asperata is the only legume described so far to exclusively associates with Cupriavidus symbionts. Moreover, strain AMP6 represents an early-diverging lineage within the symbiotic Cupriavidus group and has the capacity to develop an effective nitrogen-fixing symbiosis with three other species of Mimosa. Therefore, the genome of Cupriavidus sp. strain AMP6 enables comparative analyses of symbiotic trait evolution in this genus and here we describe the general features, together with sequence and annotation. The 7,579,563 bp high-quality permanent draft genome is arranged in 260 scaffolds of 262 contigs, contains 7,033 protein-coding genes and 97 RNA-only encoding genes, and is part of the GEBA-RNB project proposal.

show abstract

Physiological and symbiotic diversity of Cupriavidus necator strains isolated from nodules of Leguminosae species

Cited by 16 publications

References 49 publications

Ocorrência e eficiência do microssimbionte de Sesbania virgata(Cav.) Pers. em função das propriedades do solo

Ocorrência e eficiência do microssimbionte de Sesbania virgata(Cav.) Pers. em função das propriedades do solo

Bioremediation of copper in sediments from a constructed wetland ex situ with the novel bacterium Cupriavidus basilensis SRS

High-quality permanent draft genome sequence of the Mimosa asperata - nodulating Cupriavidus sp. strain AMP6

Contact Info

Product

Resources

About