Genetic similarity of Brazilian hull-less and malting barley varieties evaluated by RAPD markers

Abstract

Barley (Hordeum vulgare L.) is widely used for brewing and animal feed. Recently, it has become desirable for human consumption due to its high nutritional significance, specially hull-less or naked barley. There are differences in nutritional and malting characteristics among barley varieties. RAPD procedure is able to separate barley varieties at various similarity levels. The aim of this work was the RAPD analysis of six Brazilian hull-less varieties and seven malting varieties. PCR reactions were performed with eleven random primers. A total of 34 RAPD fragments was obtained with five primers. A dendrogram was constructed based on the Jaccard similarity coefficient. Barley varieties displayed an average similarity coefficient of 0.53. Intravarietal monomorphic fragments allowed differentiation among varieties.

Salt and Water Deficit Tolerance in some Vicia faba L. Genotypes in Relation to Pigments, ISSR – PCR Markers and Stress Tolerance Indices

“…The percentage of similarity between the studied genotypes revealed that, the maximum value of similarity is 82.8% observed between NBL-Mar.3 and NBL-5 genotypes, whereas the minimum value is 67.1% observed between NBL-Mar.3 and Misr-1 genotypes. Similar results were reported by Kroth et al (2005), Brantestam et al (2007), Afiah et al (2010) and Afiah et al (2013) .…”

Assessment of genetic diversity in Brazilian barley using SSR markers

“…The intravarietal variability was also detected by RAPD and isoenzymes, although in lower frequency ( Selbach and Cavalli-Molina, 2000 ; Maris AF, 1992, Bachelor's thesis, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS). However, a great genetic similarity was found among cultivars ( Echart-Almeida and Cavalli-Molina, 2000 ; Kroth et al , 2005 ; Maris AF, 1992, Bachelor's thesis, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS). Another good option for cultivar characterization is the use of microsatellite markers (SSR) that possess high levels of polymorphism, are multiallelic and spread throughout the genome ( Gupta et al , 1996 ; Oliveira et al , 2006 ).…”

Medical Foods from Natural Sources

“…Although varietal variations in malting characteristics of barley (Allison, 1986;Bhatty, 1996;Briennan et al, 1997;Kroth et al, 2005), wheat (Weseley, 1993), sorghum (Kumar et al 1992), and finger millet (Malleshi and Desikachar, 1979;Gimbi and Kitabatake, 2002) have been reported, such information on rice is scanty.…”