Putative metabolic pathway of mannitol and sorbitol and in sugarcane

Marino, Celso Luís; Leite, Susi Meire Maximino; Farro, Ana Paula Cazerta; Sassaki, Flávio Tetsuo; Campos, Henrique Luis Veronezi de; Coscrato, Virgínia E.

doi:10.1590/s0103-90162003000400017

Cited by 3 publications

(2 citation statements)

References 9 publications

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“…For the redistribution of this nutrient, the plant must have compounds such as polyols (alcohols derived from sugars as sorbitol, mannitol and dulcitol) in the phloem. With the objective of finding sequences in the SugarCane EST Genome Project (SUCEST) data bank that codify enzymes active in the metabolic pathways of sorbitol and mannitol, Marino et al (2003) verified, by means of the comparison of enzymes of other organisms, the similarity of 18 "contigs" of sugar cane (Saccharum spp.) with 11 enzyme sequences that compose the probable metabolic pathway of fructosederived sorbitol and mannitol.…”

Section: Resultsmentioning

confidence: 99%

Utilization of Boron (10B) derived from fertilizer by sugar cane

Franco¹,

Vitti²,

Otto³

et al. 2009

Rev. Bras. Ciênc. Solo

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Section: Resultsmentioning

confidence: 99%

Utilization of Boron (10B) derived from fertilizer by sugar cane

Franco¹,

Vitti²,

Otto³

et al. 2009

Rev. Bras. Ciênc. Solo

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“…There is evidence that some Brazilian sugarcane cultivars can synthesize sorbitol naturally (Marino et al ., 2003). Therefore, the S6PDH‐like enzyme activity detected in control plants (Table 5) may be caused by a native S6PDH.…”

Section: Resultsmentioning

confidence: 99%

Growth and metabolism in sugarcane are altered by the creation of a new hexose‐phosphate sink

Chong

Bonnett

Glassop

et al. 2007

Plant Biotechnology Journal

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SummaryAn efficient in planta sugarcane-based production system may be realized by coupling the synthesis of alternative products to the metabolic intermediates of sucrose metabolism, thus taking advantage of the sucrose-producing capability of the plant. This was evaluated by synthesizing sorbitol in sugarcane (Saccharum hybrids) using the Malus domestica sorbitol-6-phosphate dehydrogenase gene (mds6pdh). Mature transgenic sugarcane plants were compared with untransformed sugarcane variety Q117 by evaluation of the growth, metabolite levels and extractable activity of relevant enzymes. The average amounts of sorbitol detected in the most productive line were 120 mg/g dry weight (equivalent to 61% of the soluble sugars) in the leaf lamina and 10 mg/g dry weight in the stalk pith. The levels of enzymes involved in sucrose synthesis and cleavage were elevated in the leaves of plants accumulating sorbitol, but this did not affect sucrose accumulation in the culm. The activity of oxidative reactions in the pentose phosphate pathway and the non-reversible glyceraldehyde-3-phosphate dehydrogenase reaction were elevated to replenish the reducing power consumed by sorbitol synthesis. Sorbitol-producing sugarcane generated 30%−40% less aerial biomass and was 10%−30% shorter than control lines. Leaves developed necrosis in a pattern characteristic of early senescence, and the severity was related to the relative quantity of sorbitol accumulated. When the Zymomonas mobilis glucokinase (zmglk) gene was co-expressed with mds6pdh to increase the production of glucose-6-phosphate, the plants were again smaller, indicating that glucose-6-phosphate deficiency was not responsible for the reduced growth. In summary, sorbitol hyperaccumulation affected sugarcane growth and metabolism, but the outcome was not lethal for the plant. This work also demonstrated that impressive yields of alternative products can be generated from the intermediates of sucrose metabolism in Saccharum spp.

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THE EFFECTS OF THE CULTURE MEDIA BY IN VITRO MULTIPLICATION OF 'Mr. S 25 ' ROOTSTOCK

Gallo

2017

Rev. Cien. Agr.

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O portaenxerto para Prunus Mr. S. 2/5 não produz sementes, portanto a sua multiplicação só é possível por via assexuada. Para esse tipo de material vegetal, a propagação in vitro pode ser uma estratégia viável para obter alta taxa de multiplicação em curto espaço de tempo. Sendo assim, o presente trabalho buscou identificar a melhor composição salina do meio, pH, fonte de carboidrato e concentração de AIB no meio de cultura, que proporcionam as melhores taxas de multiplicação in vitro do portaenxerto ‘Mr. S. 2/5’. Este estudo foi dividido em três experimentos, sendo todos conduzidos a partir de explantes com 1 cm, contendo 20 explantes por tratamento, cultivados em sala de crescimento a 25±2<sup>o</sup>C, fotoperíodo de 16 horas e densidade de fluxo luminoso de 48 μmol m-2s-1. As avaliações foram realizadas sempre aos 30 dias após a instalação de cada experimento. Em meio MS com pH 5,2 obteve-se o maior número de brotações por explante. Entretanto, a fonte e a concentração de carboidrato não exerceram influência sobre o número e o comprimento das brotações formadas. Quando o meio foi suplementado com 0,06 mg L<sup>-1</sup> de AIB se registrou a formação do maior número de brotações, porém sem influência significativa no comprimento das mesmas.

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Putative metabolic pathway of mannitol and sorbitol and in sugarcane

Cited by 3 publications

References 9 publications

Utilization of Boron (10B) derived from fertilizer by sugar cane

Utilization of Boron (10B) derived from fertilizer by sugar cane

Growth and metabolism in sugarcane are altered by the creation of a new hexose‐phosphate sink

THE EFFECTS OF THE CULTURE MEDIA BY IN VITRO MULTIPLICATION OF 'Mr. S 25 ' ROOTSTOCK

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