Número de ínstares larvais de Tuta absoluta (Meyrick) em genótipos de tomateiro

Giustolin, Teresinha Augusta; Vendramim, José Djair; Parra, José Roberto Postali

doi:10.1590/s0103-90162002000200028

Cited by 7 publications

(5 citation statements)

References 10 publications

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“…Interestingly, the diet that resulted better for T. absoluta larval and pupal viability, and fertility, yielded similar pupal weight to those from the D4 and natural diets, for both males and females, an indication that the nutritional conditions of the artificial diet produced similar patterns to the natural diet. Other positive aspect found in this study for the selected artificial diet D3 is that T. absoluta developed four larval instars on this diets, the same number as the insect has when it is reared on tomato leaves ( Erdoğan and Babaroğlu 2014 , Giustolin et al 2002 , Haji et al 1988 , Mihsfeldt and Parra 1999 ).…”

Section: Discussionsupporting

confidence: 53%

Selection and Suitability of an Artificial Diet forTuta absoluta(Lepidoptera: Gelechiidae) Based on Physical and Chemical Characteristics

Bajonero

Parra

2016

J Insect Sci

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Tuta absoluta (Meyrick, 1917) is a key tomato pest in South America and, recently, in Europe and Africa. To develop efficient control methods for this pest, adequate rearing protocols are desirable. As an alternative to tomato leaves (natural diet), we evaluated four artificial diets. Biological traits including larval and pupal viability and development time, pupal weight and deformations were assessed. Additionally, the optimum container size and larval density were evaluated. The diet based on casein, wheat germ and cellulose allowed the best development of T. absoluta, showing higher viability and no negative effects on larval instars and pupal weight. The best container was a glass tube measuring Ø 1 × h 6 cm, topped with waterproof cotton, with a density of three larvae. To evaluate the suitability of this diet, T. absoluta was reared during eight generations and life-table parameters were estimated for the F1, F3, F6, and F8 generations. The total viability (egg–adult) increased over the generations, reaching 75% in the eighth generation. Based on life-table estimations no differences among generations were found. The net reproductive rate (Ro) was higher than 40, the intrinsic rate of increase (rm) ranged between 0.08 and 0.11, the finite rate of increase (λ) was 1.1, the mean generation time (T) have a maximum of 44 d and doubling time ranged from 5.89–8.32 generations. These results indicated that a diet based on casein, wheat germ and cellulose was suitable for T. absoluta rearing in laboratory conditions.

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Section: Discussionsupporting

confidence: 53%

Selection and Suitability of an Artificial Diet forTuta absoluta(Lepidoptera: Gelechiidae) Based on Physical and Chemical Characteristics

Bajonero

Parra

2016

J Insect Sci

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“…Total RNA was extracted separately from whole individuals (eggs, adults, and larval bodies at various stages). Larval staging in T. absoluta requires the measurement of the cephalic capsule [ 41 ], so we putatively categorized individuals by larval length and days post-emergence (DPE) as 1 st stage (0.6-1.7 mm long; emergence to 3–4 DPE), 2 nd stage (1.2-3.0 mm long; 5–6 DPE), 3 rd stage (2.1-5.0 mm long; 7–8 DPE) and 4 th stage (3.9-8.0 mm long; 10–11 DPE). To obtain approximately 50–100 mg of fresh weight, RNA was extracted from ca.…”

Section: Methodsmentioning

confidence: 99%

De novo transcriptome assembly and analysis to identify potential gene targets for RNAi-mediated control of the tomato leafminer (Tuta absoluta)

et al. 2015

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BackgroundProviding double-stranded RNA (dsRNA) to insects has been proven to silence target genes, and this approach has emerged as a potential method to control agricultural pests by engineering plants to express insect dsRNAs. A critical step of this technology is the screening of effective target genes essential for insect development and/or survival. The tomato leafminer (Tuta absoluta Meyrick) is a major Solanum lycopersicum (tomato) pest that causes significant yield losses and has recently invaded Europe, from where it is spreading at an alarming rate. To explore RNA interference (RNAi) against T. absoluta, sequence information on potential target genes is necessary, but only a few sequences are available in public databases.ResultsWe sequenced six libraries from RNA samples from eggs, adults, and larvae at four stages, obtaining an overall total of around 245 million reads. The assembled T. absoluta transcriptome contained 93,477 contigs with an average size of 1,574 bp, 59.8 % of which presented positive Blast hits, with 19,995 (21.4 %) annotated by gene ontology. From the transcriptome, most of the core genes of the RNAi mechanism of Lepidoptera were identified indicating the potential suitability of T. absoluta for gene silencing. No contigs displayed significant similarity with a RNA-dependent RNA Polymerase. Genes from the juvenile hormone and ecdysteroid biosynthetic pathways were identified, representing potential target genes for systemic silencing. Comparisons of transcript profiles among stages revealed 1,577 genes differentially expressed at earlier larval stages, from which potential gene targets were identified. Five of these genes were evaluated using in vitro transcribed dsRNA absorbed by tomato leaflets, which were fed to 1st instar T. absoluta larvae, resulting in significant reduction of larval body weight while exhibiting significant knockdown for three of the genes.ConclusionsThe transcriptome we generated represents a valuable genomic resource for screening potential gene targets that affect the development or survival of T. absoluta larvae. Five novel genes that showed greater expression at the 1st larval stage were demonstrated to be effective potential RNAi targets by reducing larval weight and can be considered good candidates for use in RNAi-mediated crop protection.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-015-1841-5) contains supplementary material, which is available to authorized users.

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“…Five first instar T. absoluta caterpillars were uniformly distributed on the abaxial surface of leaflets. The insects used were from the mass rearing in the laboratory (Giustolin et al, 2002). The treatments were obtained by variables: leaves injured (LED), and injured on plant (DPL).…”

Section: Methodsmentioning

confidence: 99%

Fontes De Resistência a Traça Do Tomateiro Em Tomate Cereja

Sobreira¹,

Andrade²,

Almeida³

et al. 2009

RSA

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The tomato plant has narrow genetic base due to intensive breeding and domestication of wild varieties. On the other hand, accessions are a potential source of germplasm against insect pests for tomato crop. The aim of this study was to evaluate the injury caused by Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) in the leaves and plant of Lycopersicon esculentum var. cerasiforme. The phenotypic and qualitative variability among 15 accessions, injury in leaves and plant after 20 days were evaluated. The injury in leaves and plant were carried out by the scale notes. In general, the accession CCAUFES 40 was the most resistant to T. absoluta, what indicate that one have potential as a source of germplasm for plants breeding of tomato against T. absoluta.Key-words: Tuta absoluta; germplasm; varietal resistance; breeding. RESUMOO tomateiro possui estreita base genética, devido ao intensivo melhoramento e domesticação de espécies selvagens. Por outro lado, acessos são fontes potenciais de germoplasma contra insetos-praga dessa cultura. O objetivo deste estudo foi avaliar os danos causados por Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) em folhas e nas plantas de Lycopersicon esculentum var. cerasiforme. A variabilidade fenotípica e qualitativa entre 15 acessos, os danos nas folhas e plantas após 20 dias foram avaliados. O dano nas folhas e plantas foi avaliado por escala de notas. De maneira geral, o acesso CCAUFES 40 foi a mais resistente a T. absoluta, o que indica que o mesmo possui potencial como fonte de germoplasma para o melhoramento de plantas de tomate contra T. absoluta.

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Número de ínstares larvais de Tuta absoluta (Meyrick) em genótipos de tomateiro

Cited by 7 publications

References 10 publications

Selection and Suitability of an Artificial Diet forTuta absoluta(Lepidoptera: Gelechiidae) Based on Physical and Chemical Characteristics

Selection and Suitability of an Artificial Diet forTuta absoluta(Lepidoptera: Gelechiidae) Based on Physical and Chemical Characteristics

De novo transcriptome assembly and analysis to identify potential gene targets for RNAi-mediated control of the tomato leafminer (Tuta absoluta)

Fontes De Resistência a Traça Do Tomateiro Em Tomate Cereja

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