ANTAGONISMO IN VITRO DE Trichoderma spp.  A Verticillium dahliae KLEB

Martins-Corder, Maisa Pimentel; Melo, I. S. de

doi:10.1590/s0103-90161998000100002

Cited by 29 publications

(17 citation statements)

References 17 publications

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“…This isolate was aggressive, giving to that antagonist an enormous ability as a mycoparasite by means of the fast and intense colonization of the substrate, leading to the destruction of the cellular wall, probably to obtain nutrients from the dead cells. This observation matched the report by Martins-Corder and Melo (1998), where they proved the in vitro antagonism of Trichoderma spp. (Verticillium dahlia).…”

Section: Resultssupporting

confidence: 92%

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Antagonism of fungi with biocontrol potential of papaya black spot caused by Asperisporium caricae

Vivas¹,

Silveira²,

Santos³

et al. 2018

Aust J Crop Sci

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The antifungal activity of fungi with biocontrol potentials should be studied. Therefore, this study aimed at establishing alternative agent to replace the chemical control of papaya black spot. We investigated the antagonistic activity of the fungi Hansfordia pulvinata (H-611), Acremonium sp (A-602, A-617 and A-598), Simplicillium lanosoniveum (S-599), Lecanicillium lecanii (L-622), and Sarocladium implicatum (I-609) on the phytopathogenic fungus A. caricae, by evaluating the mycoparasitism, the enzymatic activity and the production of volatile and non-volatile compounds. For the evaluation of mycoparasitism, each antagonic fungi isolate was microcultured along with conidia of A. caricae. Then, events of mycoparasitism were evaluated at 24h, 48h, 72h, and 96h after inoculation under an optical microscope. We estimated the enzymatic activity (protease and lipase) of the fungi in a particular culture medium for each enzyme. The activity of the protease was reflected in the mean diameter of the halo. The lipase activity was measured by the halo/colony relation. In order to detect volatile compounds in the antibiosis test, A. caricae was cultured under a medium along with the isolates described. For the test of non-volatile compounds, we evaluated the germination of A. caricae in culture medium by diffusion under cellophane paper. As a result, the mycoparasitism of the H-611, A-602, A-617, A-598, S-599, and L-622 isolates was confirmed on the microcultures. We observed higher activities of protease in the I-609, S-599, A-602, and A-598 isolates. The H-611 and A-602 isolates presented higher activities for lipase. We did not observe effect of volatile compounds in any of the tested isolates. In the non-volatile compound test, there was production of antifungal metabolite highlighting the A-617 isolate, which inhibited the germination of the conidia of A. cariae by 100%.

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Section: Resultssupporting

confidence: 92%

“…Some authors explained that the volatile antibiotic acts on the susceptible fungi by means of the inhibition of the mycelial growth. This means that isolate with ability to produce nonvolatile substances do not always produce such volatile substances (Martins-Corder and Melo, 1998).…”

Section: Action Of Volatile and Non-volatile Compounds On Conidial Gementioning

confidence: 99%

Antagonism of fungi with biocontrol potential of papaya black spot caused by Asperisporium caricae

Vivas¹,

Silveira²,

Santos³

et al. 2018

Aust J Crop Sci

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“…Isaias et al (2014) found that only volatile compounds produced by T. harzianum, T. koningiopsis, and T. asperellum inhibited the growth of Sclerotium rolfsii and Verticillium dahliae colonies, with values significantly higher than 60% and between 40% and 60%, respectively. Martins-Corder & Melo (1998) found that 4 out of 7 Trichoderma spp. isolates produced volatile compounds with higher inhibitory effect on the growth of V. dahliae colony although there were no statistical differences between them.…”

Section: Effect Of Carbon Sources On the Production Of Volatile Compomentioning

confidence: 99%

Production of antifungal compounds by Trichoderma spp. to control Phytophthora nicotianae, causal agent of gummosis on citrus

Melo¹,

Martins²,

Kupper³

2015

Citrus Research &Amp; Technology

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Orange crops in Brazil are affected by various diseases such as gummosis caused by Phytophthora nicotianae, which is controlled by chemical fungicides, by preventive nature through cultural practices and utilization of tolerant rootstock. However, the high costs and environmental problems caused by the intensive use these fungicides have led to the search for alternative methods of control. The objective this study was to evaluate the in vitro antagonistic activity of Trichoderma spp. isolates against P. nicotianae by the production of antimicrobial compounds and an alfalfa seedling bioassay, thus, 12 out of the 50 isolates of Trichoderma spp. evaluated were selected based on in vitro screening to compose bioassay treatments. The paired culture showed that all isolates tested inhibited the mycelial growth of the pathogen. In the production of antimicrobial compounds, 41 isolates produced volatile compounds using dextrose as carbon source; however, further assays showed that the use of sucrose or maltose as carbon source increases the production of these compounds. Seven isolates of Trichoderma spp. produced thermostable compounds and 14 isolates produced cell-free antimicrobial compounds of the antagonist. In the alfalfa seedling bioassay, four isolates of Trichoderma spp. inhibited the formation of sporangia and mycelia growth. The in vitro evaluation concerning to the antimicrobial compound production by the Trichoderma spp. isolates and the alfalfa seedling bioassay were able to select biocontrol agents to control P. nicotianae. The four most promising isolates were TB12, TB14, TB28, and TB30 and the mechanisms of action that may be involved in the biocontrol are production of volatile compounds and cell-free filtrates by the antagonists.

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“…Vale salientar também que os mecanismos de ação avaliados no teste de cultura pareada, tais como a rápida colonização de um patógeno habitante do solo, podem variar entre isolados da mesma espécie (MARTINS-CORDER; MELO, 1998;CARVALHO et al, 2011c (1) Médias seguidas pelas mesmas letras minúsculas nas colunas e maiúsculas nas linhas não diferem estatisticamente entre si, segundo teste de ScottKnott (p≤0,05).…”

Section: Resultsunclassified

Antagonismo de Trichoderma harzianum a Sclerotium rolfsii nas culturas do feijoeiro e soja.

2013

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Resumo -Os fungicidas sintéticos embora sejam eficientes no controle de fungo fitopatogênicos, apresentam potencial efeito tóxico à saúde humana e ao meio ambiente. Nesse contexto, o controle biológico tem sido utilizado como alternativa para reduzir os prejuízos causados por Sclerotium rolfsii em diversas culturas de importância econômica no Brasil. Sendo assim, objetivou-se com este trabalho estudar o potencial de 23 isolados de Trichoderma harzianum provenientes de solo de Cerrado como agente de controle biológico de S. rolfsii, importante patógeno nas culturas do feijoeiro e soja. A ação antagonista dos isolados foi verificada por meio do pareamento de culturas sob três temperaturas (22ºC, 25ºC e 28ºC) e em experimentos conduzidos em casa de vegetação, com as culturas de feijoeiro e soja. Os isolados CEN155, CEN158, CEN169, CEN170, CEN194 e CEN197 destacaram-se quanto à inibição do crescimento micelial de S. rolfsii, nas três temperaturas empregadas nos experimentos in vitro, ocupando pelo menos 66% da superfície do meio em placa de Petri (90 mm) e também na supressão in vivo de S. rolfsii nos experimentos com as duas culturas em casa de vegetação, proporcionando percentual médio de plantas sadias acima de 88%, aos 15 dias após as inoculações de patógeno e antagonistas no solo. Palavras-chave -Glycine max. Phaseolus vulgaris. Podridão radicular. Controle biológico.Abstract -Although synthetic fungicides have been efficient for controlling phytopathogenic fungi, these products present potential toxic effect to the human health and to environment. In this context, the biological control has been used as an alternative to reduce losses caused by Sclerotium rolfsii in several crops of economic importance in Brazil. Therefore, the objective of this work was to study 23 isolates of Trichoderma harzianum obtained from Cerrado soils as biological control agent against S. rolfsii, an important soil-borne pathogen of bean and soybean crops. The antagonism of the isolates was verified by dual culture assays (at 22ºC, 25ºC and 28ºC) and in vivo experiments carried out in greenhouse, using common bean and soybean plants. The isolates CEN155, CEN158, CEN169, CEN170, CEN194 and CEN197 were able to inhibit the mycelial growth of S. rolfsii in the three temperatures employed in the dual culture assays, colonizing at least 66% of the Petri dish surfaces and were also effective for the in vivo controlling of S. rolfsii on bean and soybean crops, affording average value (%) of healthy plants over 88%, at 15 days after soil inoculations with pathogen and antagonists.

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ANTAGONISMO IN VITRO DE Trichoderma spp. A Verticillium dahliae KLEB

Cited by 29 publications

References 17 publications

Antagonism of fungi with biocontrol potential of papaya black spot caused by Asperisporium caricae

Antagonism of fungi with biocontrol potential of papaya black spot caused by Asperisporium caricae

Production of antifungal compounds by Trichoderma spp. to control Phytophthora nicotianae, causal agent of gummosis on citrus

Antagonismo de Trichoderma harzianum a Sclerotium rolfsii nas culturas do feijoeiro e soja.

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