Ácido ascórbico como agente redutor para determinação de fósforo por colorimetria

Santos, Lenio da Silva; Silva, Leandro Souza da Departamento de Solos; Griebeler, Gustavo

doi:10.1590/s0103-84782014000600010

Cited by 5 publications

(3 citation statements)

References 7 publications

(5 reference statements)

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“…To eliminate the interference of phosphorus and other elements in the determination of silicon, organic acids, such as tartaric and oxalic acids, are generally added (Nolla et al, 2010). In the phosphorus analysis, ascorbic acid is used as a reducing agent for the phosphate reaction with molybdate in an acid medium (Santos;Silva;Griebeler, 2014).…”

Section: Resultsmentioning

confidence: 99%

Phosphorus availability in soil amended with biochar from rice rusk and cattle manure and cultivated with common bean

et al. 2020

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The soils of the Brazilian Savanna are generally acidic and have low availability of nutrients, so the use of alternative inputs to improve their fertility should be investigated. The objectives of this study were to evaluate the potential of biochars from rice husk (BHR) and from bovine manure (BCM) in increasing phosphorus availability and their effects on soil chemical properties and in common beans plants. The experiments were carried out in a completely randomized design, in a 4x2+3 factorial scheme with four replicates. The treatments were four biochar doses (1, 2, 3 and 4% m/v), two biochars (BRH and BCM) and three additional treatments (C1, no liming and no fertilization; C2, addition of Ca and Mg carbonate and NPK fertilizers and; C3, addition of Ca and Mg silicate and NK fertilizers). In the highest doses of BRH there was an increase of 2.7, 5.3 and 2.5 times in the P content extracted by Mehlich 1 and quantified by colorimentria, by Mehlich 1 and quantified by spectroscopy and by ion exchange resin and quantified by spectroscopy, respectively. For the highest doses of BCM, the increases in P content were 51.3, 289.2 and 88.4 times greater than in C1, respectively, according to the methods described for BRH. The biochars increased soil pH, CEC, nutrient content and the growth of bean plants compared to C1, especially BCM. However, the production of dry matter was significantly lower than that obtained in C2.

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Section: Resultsmentioning

confidence: 99%

Phosphorus availability in soil amended with biochar from rice rusk and cattle manure and cultivated with common bean

et al. 2020

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“…After image acquisition, the seedling roots and shoots were each dried to a constant weight at 65°C in order to obtain shoot dry weight (SDW), root dry weight (RDW), and total dry weight (TDW). The shoot P content (SPC) and root P content (RPC) were measured by the blue molybdenum spectrophotometry method (Pradhan and Pokhrel, 2013;Santos et al, 2014).…”

Section: Methodsmentioning

confidence: 99%

Screening of inbred popcorn lines for tolerance to low phosphorus

et al. 2016

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ABSTRACT. Increasing phosphorus use efficiency in agriculture is essential for sustainable food production. Thus, the aims of this study were: i) to identify phosphorus use efficiency (PUE) in popcorn lines during the early plant stages, ii) to study the relationship between traits correlated with PUE, and iii) to analyze genetic diversity among lines. To accomplish this, 35 popcorn lines from Universidade Estadual de Maringá breeding program were studied. The experiment was conducted in a growth chamber using a nutrient solution containing two concentrations of phosphorus (P): 2.5 µM or low P (LP) and 250 µM or high P (HP). After 13 days in the nutrient solution, root morphology traits, shoot and root dry weight, and P content of the maize seedlings were measured. A deviance analysis showed there was a high level of genetic variability. An unweighted pair group method with arithmetic mean (UPGMA) clustering analysis identified three groups for the LP treatment (efficient, intermediate, and inefficient) and three groups for the HP treatment (responsive, moderately responsive, and unresponsive). The results of a principal component analysis and selection index were consistent with the UPGMA analysis, and lines 1, 2, 13, 17, 26, and 31 were classified as PUE.

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“…After drying, the leaf and stem samples were ground for quantification of the phosphorus concentration in 1 g of dry matter. For this, extraction was performed by acid digestion (HNO 3 and H 2 O 2 ), and P was determined by spectrophotometry (Specord 2010, Analytik Jena, Jena, Germany) using the molybdate method . Total phosphorus content was then determined by multiplying the concentration of phosphorus in 1 g of dry matter by the corresponding dry weight (mg P/plant).…”

Section: Experimental Proceduresmentioning

confidence: 99%

Unraveling the Mechanisms of Efficient Phosphorus Utilization in Popcorn (Zea mays L. var. everta): Insights from Proteomic and Metabolite Analysis

Barboza Bispo,

Teixeira do Amaral,

Pinto

et al. 2024

J. Proteome Res.

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The expansion of agriculture and the need for sustainable practices drives breeders to develop plant varieties better adapted to abiotic stress such as nutrient deficiency, which negatively impacts yields. Phosphorus (P) is crucial for photosynthesis and plant growth, but its availability in the soil is often limited, hampering crop development. In this study, we examined the response of two popcorn inbred lines, L80 and P7, which have been characterized previously as P-use inefficient and P-use efficient, respectively, under low (stress) and high P (control) availability. Physiological measurements, proteomic analysis, and metabolite assays were performed to unravel the physiological and molecular responses associated with the efficient use of P in popcorn. We observed significant differences in protein abundances in response to the P supply between the two inbred lines. A total of 421 differentially expressed proteins (DEPs) were observed in L80 and 436 DEPs in P7. These proteins were involved in photosynthesis, protein biosynthesis, biosynthesis of secondary metabolites, and energy metabolism. In addition, flavonoids accumulated in higher abundance in P7. Our results help us understand the major components of P utilization in popcorn, providing new insights for popcorn molecular breeding programs.

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Ácido ascórbico como agente redutor para determinação de fósforo por colorimetria

Abstract: RESUMO Em muitos laboratórios de análises de solos, o ácido 1-Amino-2-naftol-4-sulfônico (ANS) é usado como agente redutor ABSTRACT In soil laboratory testing, the acid 1-amino-2-naphthol-4-sulfonic (ANS) is used as reducing agent to determine

Cited by 5 publications

References 7 publications

Phosphorus availability in soil amended with biochar from rice rusk and cattle manure and cultivated with common bean

Phosphorus availability in soil amended with biochar from rice rusk and cattle manure and cultivated with common bean

Screening of inbred popcorn lines for tolerance to low phosphorus

Unraveling the Mechanisms of Efficient Phosphorus Utilization in Popcorn (Zea mays L. var. everta): Insights from Proteomic and Metabolite Analysis

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