Avian IgY antibodies: characteristics and applications in immunodiagnostic

Munhoz, Lívia Silveira; Vargas, Gilberto D’Ávila; Fischer, Geferson; Lima, Marcelo de; Esteves, Paulo Augusto; Hübner, Sílvia de Oliveira

doi:10.1590/s0103-84782014000100025

Cited by 47 publications

(33 citation statements)

References 37 publications

(60 reference statements)

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“…Both polyclonal and monoclonal antibodies may be good options in this regard. The polyclonal immunoglobulin yolk (IgY) antibodies produced in chicken laying eggs have various advantages, such as high affinity for detection, multiple epitope recognition [33], low cost, and ease in purification compared to blood collection [34]. On the other hand, monoclonal-derived antibodies are also good tools for detection because they demonstrate specific binding affinity to their targets (ZIKV_E proteins).…”

Section: Discussionmentioning

confidence: 99%

Expression, Purification, and Characterization of Anti-Zika virus Envelope Protein: Polyclonal and Chicken-Derived Single Chain Variable Fragment Antibodies

Mwale

Lee

Lin

et al. 2020

IJMS

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Zika virus (ZIKV) is a new and emerging virus that has caused outbreaks worldwide. The virus has been linked to congenital neurological malformations in neonates and Guillain–Barré syndrome in adults. Currently there are no effective vaccines available. As a result, there is a great need for ZIKV treatment. In this study, we developed single chain variable fragment (scFv) antibodies that target the ZIKV envelope protein using phage display technology. We first induced an immune response in white leghorn laying hens against the ZIKV envelope (E) protein. Chickens were immunized and polyclonal immunoglobulin yolk (IgY) antibodies were extracted from egg yolks. A high-level titer of anti-ZIKV_E IgY antibodies was detected using enzyme-linked immunosorbent assay (ELISA) after the third immunization. The titer persisted for at least 9 weeks. We constructed two antibody libraries that contained 5.3 × 106 and 4.5 × 106 transformants. After biopanning, an ELISA phage assay confirmed the enrichment of specific clones. We randomly selected 26 clones that expressed ZIKV scFv antibodies and classified them into two groups, short-linker and long-linker. Of these, four showed specific binding activities toward ZIKV_E proteins. These data suggest that the polyclonal and monoclonal scFv antibodies have the diagnostic or therapeutic potential for ZIKV.

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Section: Discussionmentioning

confidence: 99%

Expression, Purification, and Characterization of Anti-Zika virus Envelope Protein: Polyclonal and Chicken-Derived Single Chain Variable Fragment Antibodies

Mwale

Lee

Lin

et al. 2020

IJMS

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“…Currently, there are several methods to isolate and purify functional active chicken IgY from egg yolk, even for large scale purification (Munhoz et al, 2014). These methods can be performed by salt precipitation (Deignan et al, 2000;Chacana et al, 2003), chromatographic techniques (Meulenaer and Huyghebaert, 2001), and ultrafiltration (Kim and Nakai, 1998).…”

Section: Discussionmentioning

confidence: 99%

“…IgY technology has been used in many medical areas, especially for diagnosis purposes. Specific chicken antibodies have been successfully used against a wide variety of antigens (Munhoz et al, 2014). Various authors reported the efficiency, sensitivity, and specificity of diagnostic tests using IgY from various microorganisms such as Toxoplasma gondii, Schistosoma japonicum, Trichinella spiralis and coronavirus (Ferreira Júnior et al, 2012;Cai et al, 2012;Wang et al, 2012;Palaniyappan et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Avian antibodies (IgY) against Trypanosoma cruzi : Purification and characterization studies

Grando

Baldissera

Sá

et al. 2017

Journal of Immunological Methods

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Trypanosoma cruzi is a flagellated protozoan belonging to the Trypanosomatidae family, the etiologic agent of Chagas disease. Currently, there is neither a licensed vaccine nor effective treatment, characterizing an unmet clinical need. The IgY refers to the egg yolk immunoglobulin (Y=yolk) and its production and use are subjects of many studies due to the diversity of its diagnostic and therapeutic applications. Several researchers have shown that the use of specific IgY may prevent and/or control infectious and parasitic diseases. Based on these evidences, the aim of this study was to immunize chickens with trypomastigotes of T. cruzi in order to produce highly effective and pure antibodies (IgY), as well as extract, characterize, quantify, and verify cytotoxic effects of IgY anti-T. cruzi. After the induction of IgY production by chickens, the eggs were collected and the IgY was extracted by method of precipitation of polyethylene glycol 6000. The IgY anti-T. cruzi characterization was performed using polyacrylamide gel electrophoresis (SDS-PAGE), western-blot and enzyme-linked immunosorbent assay (ELISA). Moreover, the cytotoxic or proliferative effects of IgY anti-T. cruzi was verified by MTT assay. The concentration of IgY in yolk was 8.41±1.47mg/mL. The characterization of IgY reveled bands of stained peptides with molecular weight between 75 and 50kDa and 37 and 25kDa. In the ELISA test was observed that there was antigen-antibody reaction throughout the sample period. The concentrations of 1, 5 and 10mg/mL of IgY anti-T. cruzi presented no cytotoxic of proliferative effects in mononuclear and VERO cells in vitro. The results indicated that T. cruzi is able to generate a high production of specific immunoglobulins in chickens, it did not cause damage to the cell membrane and no proliferative effect.

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“…Here we applied the cathepsin F capture ELISA for coproantigen diagnosis of human opisthorchiasis. Making use of chicken IgY, which does not cross react with mammalian immunoglobulins [34,20] as a capture antibody for native cathepsin F in the feces and a rabbit anti- Opisthorchis somatic antigens as the second antibody, we observed sensitivity greater than 90%. Stability and proper binding of chicken IgY to ELISA plate are additional advantages of utilizing this antibody as a capture antibody [35].…”

Section: Discussionmentioning

confidence: 99%

“…Chicken IgY antibody, the major serum immunoglobulin in birds, has functions similar to mammalian IgG [20]. It is a stable immunoglobulin that can be simply obtained from egg yolk with high quantity with no necessity to bleed the laboratory animal.…”

Section: Introductionmentioning

confidence: 99%

Chicken IgY-based coproantigen capture ELISA for diagnosis of human opisthorchiasis

Teimoori

Arimatsu

Laha

et al. 2017

Parasitology International

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Diagnosis of Opisthorchis viverrini infection by conventional stool examination is increasingly difficult due to the low intensity of the infection after several rounds of control programmes in endemic regions as well as coinfections with intestinal flukes. Therefore sensitive and specific diagnostic test is needed. In this study, a coproantigen sandwich ELISA using recombinant O. viverrini cathepsin F (rOv-CF) was developed. This sandwich ELISA employing chicken IgY raised against rOv-CF in combination with rabbit IgG antibody to the somatic O. viverrini antigens showed a lower detection limit (LLD) of 70 ng native O. viverrini somatic antigens by spiking the parasite antigens into control feces. When applied to the diagnosis, the IgY-based sandwich ELISA exhibited sensitivity and specificity of 93.3% and 76.7%, respectively, in an investigation of 90 human cases positive or negative for opisthorchiasis. The positive predictive value (PPV) and negative predictive value (NPV) for this coproantigen detection were 66.7% and 95.2%, respectively. This IgY-based sandwich ELISA using parasite cathepsin F detection shows a promising immunodiagnostic alternative for human opisthorchiasis in endemic regions.

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Avian IgY antibodies: characteristics and applications in immunodiagnostic

Abstract: Immunoglobulin Y (IgY

Cited by 47 publications

References 37 publications

Expression, Purification, and Characterization of Anti-Zika virus Envelope Protein: Polyclonal and Chicken-Derived Single Chain Variable Fragment Antibodies

Expression, Purification, and Characterization of Anti-Zika virus Envelope Protein: Polyclonal and Chicken-Derived Single Chain Variable Fragment Antibodies

Avian antibodies (IgY) against Trypanosoma cruzi : Purification and characterization studies

Chicken IgY-based coproantigen capture ELISA for diagnosis of human opisthorchiasis

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