Kit comercial de ELISA® para a detecção de coproantígenos e exame coproparasitológico em bovinos com fígados condenados por fasciolose

Abstract

RESUMO Objetivou Palavras-chave: Fasciola hepatica, bovino, imunodiagnóstico. ABSTRACT The aim of this study was to compare a commercial ELISA kit for detection on coproantigen examination and fecal sedimentation using as gold standard inspection diagnosis of bovine livers at slaughter. In addition, we evaluated the correlation between the measured intensity of infection by counting eggs in the feces

Confirmation of Fasciola hepatica resistant to triclabendazole in naturally infected Australian beef and dairy cattle

“…Analysis of frozen faecal samples has been shown in sheep ( Flanagan et al, 2011a ) and cattle ( Brockwell et al, 2013 ) to not affect coproantigen quantitation. Several studies using the BIOX coproantigen kit have adhered to the manufacturer’s instructions using a cut-off for positivity of 0.15 OD or equivalent when expressed as a percentage of the supplied control antigen ( Martínez-Valladares et al, 2010; Flanagan et al, 2011b; Bernardo et al, 2012; Novobilsky et al, 2012; Robles-Pérez et al, 2013 ). However, Novobilsky et al (2012) expressed concerns as to the high rate of false negatives when using the BIOX kit’s recommended negative cut-off point for positivity and called for further verification of the ELISA’s sensitivity.…”