Fatores prognósticos e preditivos dos tumores caninos definidos com auxílio da imuno-histoquímica

Horta, Rodrigo dos Santos; Costa, Mariana de Pádua; Lavalle, Gleidice Eunice; Araújo, Roberto Baracat de; Cassali, Geovanni Dantas

doi:10.1590/s0103-84782012000600013

Cited by 8 publications

(15 citation statements)

References 36 publications

(47 reference statements)

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“…The determination of the immunophenotype of lymphoma is of undeniable importance, since it allows detailed diagnosis and prognosis and directs the choice of a more appropriate treatment (Horta et al 2012). In a study by Kiupel et al (1999) using the same antibodies from the present study, it was demonstrated that the determination of the immunophenotype in dogs with lymphoma was important for the prognosis of survival, indicating that dogs with B-cell lymphomas, which are less aggressive, were those with a longer survival time (Teske et al 1994).…”

Section: Discussionmentioning

confidence: 99%

Canine lymphomas diagnosed in southern Brazil from 2000 to 2017: epidemiology and immunophenotype

et al. 2019

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Lymphoma is a neoplasm that originates from solid hematopoietic tissues and is one of the most common tumors in dogs. The goal of the present study was to perform a retrospective study of canine lymphomas diagnosed at the “Laboratório Regional de Diagnóstico”, at the “Faculdade de Veterinária” of the “Universidade Federal de Pelotas” (LRD-UFPel) from 2000 to 2017, to determine the epidemiology and anatomical distribution, and to evaluate the histopathological and immunohistochemical aspects of each case according to the adapted Kiel classification. The protocols for necropsies and biopsies in the laboratory were reviewed. Lymphoma was diagnosed in 77 dogs. Approximately 37.7% (29/77) of affected dogs had no defined breed, while dogs with defined breeds accounted for 58.4% (45/77) of the diagnoses. The occurrence in males (40/77) was slightly higher than that in females (36/77), and the mean age was 8.1 years (1.4-17 years). The most affected age group was between six and 10 years of age with 31 cases (40.2%). Regarding the anatomical classification, the multicentric form was the most prevalent, accounting for 71.4% (55/77) of the diagnoses. In 40 cases that immunophenotyping was performed, B-cell lymphomas represented 62.5% of the diagnoses (25/40), while T-cell lymphomas corresponded to 37.5% of the diagnoses (15/40). The degree of malignancy according to the modified Kiel classification was low in 35% of lymphomas (14/40) and high in 65% of cases (26/40). The multicentric form was more frequent in the region of influence of the LRD-UFPel. Identification of the immunophenotype can improve the quality of life and survival in affected dogs since it allows the most appropriate treatment for each patient.

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Section: Discussionmentioning

confidence: 99%

Canine lymphomas diagnosed in southern Brazil from 2000 to 2017: epidemiology and immunophenotype

et al. 2019

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“…Consequently, the occurrence of mammary neoplasia in domestic animals is a subject addressed worldwide (Lana et al 2007, Szczubial et al 2012, Gundim et al 2016, Dolka et al 2018, Marconato et al 2019. In addition, cancer is the leading cause of death in elderly dogs and the second largest cause in companion animals (Horta et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Relationship of obesity with clinical and laboratory changes in female dogs with mammary neoplasia

et al. 2020

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Obesity may be associated with the onset of mammary tumors in women. In companion animals, these data are still scarce, mainly associating the clinic of the patient with laboratory and histological findings. This study aimed to evaluate the clinical and laboratory aspects of female dogs with mammary neoplasia, investigating whether they are related to obesity. Four groups of spayed or non-spayed female dogs were studied, divided into (G1) female dogs without mammary tumor and normal body condition score (BCS), (G2) female dogs without mammary tumor and obese, (G3) female dogs with mammary tumor and normal BCS and (G4) female dogs with mammary tumor and obese. BCS, canine body mass index (CBMI), blood count, glucose, total cholesterol, triglycerides and cytopathological and histopathological examinations were evaluated. Mixed-breed animals were the most prevalent in this study (67.5%). There was a limitation regarding the use of CBMI related to body proportionality. Considering the 28 tumors evaluated, carcinoma in mixed tumor was the most prevalent malignant histological type, while adenomyoepithelioma was the most prevalent benign histological type. It was possible to conclude that obesity was not related to clinical and laboratory changes in the female dogs affected with mammary neoplasias. In addition, no relationship was noted between BCS and CBMI with the histological type or malignancy of mammary neoplasia, since the malignant histological types were the most prevalent tumors in both the ideal score group and the overweight to obese group.

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“…The use of the cell block technique to determine the expression of oestrogen (ER), progesterone (PR) and cytokeratin 5 (CK5) receptors allows a better therapeutic approach (Bueno y col 2013, Zanoni et al 2012Horta et al 2012). An increased expression for the oestrogen (ER) and progesterone (PR) receptors is related to a better prognosis while the positivity of the CK5 receptor is related to a worse prognosis, in both canines and humans with mammary carcinomas (Terra et al 2012, Horta et al 2012, Figueiroa et al 2012.…”

Section: Introductionmentioning

confidence: 99%

“…An increased expression for the oestrogen (ER) and progesterone (PR) receptors is related to a better prognosis while the positivity of the CK5 receptor is related to a worse prognosis, in both canines and humans with mammary carcinomas (Terra et al 2012, Horta et al 2012, Figueiroa et al 2012. In canines, the expression of ER is higher in benignant tumors than in malignant ones and is also higher in complex and mixed tumors in comparison with simple sub-types (De Las Mulas et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Cell block sensitivity for immunohistochemical detection of cytokeratin 5, oestrogen and progesterone receptors in canine primary mammary carcinoma

Silveira

Campos

Düfloth

et al. 2017

Austral j. vet. sci.

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ABSTRACT. Mammary carcinomas are relatively common ailments among female canines aged around 10 years old, presenting an important morbidity with an average survival of five years. The cytoinclusion technique is frequently employed in human medicine as the investigative method of choice as it quickly provides resources for the determination of the correct therapeutic response, however, the effectiveness of the technique in canines remains understudied in veterinary medicine. This study aims at evaluating the degree of correlation with immunohistochemical marking for cytokeratin 5 (CK5), oestrogen and progesterone receptors (ER and PR) between the cytoinclusion and the histopathology technique in mammary carcinomas. Twenty-five samples of mammary carcinoma, both for the cytoinclusion and histopathological techniques were submitted for histological processing; microscope slides were created for hematoxylin-eosin (HE) staining and the immunohistochemical technique (IHC) was assessed for the ER, PR and CK5 receptors. Through the HE staining, we reached a concordance rate of 100% between the cytoinclusion and the histopathological analysis in the diagnosis of carcinomas. The immunohistochemical assay presented sensitivity of 85.71%, 95.45% and 100% and Cohen's kappa of 0.78, 0.84 and 0.95 for ER, PR and CK5, respectively, as well as 100% specificity and P<0.01 for all three markers. Therefore, cytoinclusion represents an accessible, fast and low-cost method, offering high sensitivity for the cytomorphological and immunohistochemical diagnosis of mammary carcinoma in female canines.Key words: cytoinclusion, histopathological, immunohistochemistry, tumor. RESUMEN.Los carcinomas mamarios son relativamente comunes en hembras caninas con edades de alrededor 10 años y representan un importante factor de morbilidad para estas hembras; la técnica de citoinclusión es frecuentemente empleada en medicina humana como un método de diagnóstico que permite proveer información rápida para la determinación de una conducta terapéutica correcta, sin embargo, la efectividad de esta técnica permanece poco estudiada en medicina veterinaria. El objetivo del estudio fue evaluar el grado de correlación entre marcadores inmunohistoquímicos para citokeratina 5 (CK5), receptores de estrógenos y progesterona (RE y RP) entre la técnica de citoinclusión y la técnica histopatológica en carcinoma mamario. Veinticinco muestras de carcinoma mamario para la técnica de citoinclusión e histopatológica fueron sometidas a procesamiento histológico, láminas fueron confeccionadas para la tinción mediante hematoxilina y eosina (HE), y la técnica de inmunohistoquímica para identificar la expresión de RE, RP y CK5. Mediante la tinción de la HE fue encontrado una proporción de concordancia del 100% entre el análisis de citoinclusión e histopatológico de carcinoma mamario. El análisis inmunohistoquímico evidenció una sensibilidad del 85,71%, 95,45% y 100% y un índice kappa de Cohen de 0,78, 0,84 y 0,95 para RE, RP y CK5, respectivamente, así como 100% de especifi...

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Fatores prognósticos e preditivos dos tumores caninos definidos com auxílio da imuno-histoquímica

Cited by 8 publications

References 36 publications

Canine lymphomas diagnosed in southern Brazil from 2000 to 2017: epidemiology and immunophenotype

Canine lymphomas diagnosed in southern Brazil from 2000 to 2017: epidemiology and immunophenotype

Relationship of obesity with clinical and laboratory changes in female dogs with mammary neoplasia

Cell block sensitivity for immunohistochemical detection of cytokeratin 5, oestrogen and progesterone receptors in canine primary mammary carcinoma

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