Active and biodegradable materials have great potential in food packaging applications, improving the safety and quality of products. The objective of this study was to develop a new material based on buriti oil incorporated into a chitosan film. Different concentrations of buriti oil in dried films (2.1 g/m 2 , 10.4 g/m 2 , 20.8 g/m 2 , and 31.3 g/m 2 ) were added into a chitosan matrix (41.7 g/ m 2 ). The chitosan/buriti oil films were characterized by water-vapor barrier properties, total water-soluble matter (TSM), tensile properties, thermogravimetric analysis, microstructure, microbial permeation properties, and biodegradation estimation. The higher oil concentration improved the water-vapor barrier and the buriti oil acted largely as a plasticizer and increased the elongation at break, and decreased the tensile strength (TS) of chitosan films. The total water-soluble matter of chitosan films decreased in function of the buriti oil concentration, but the biodegradation and thermal stability increased. The chitosan films presented a microbial barrier against Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. V C 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016, 133, 43210.