Adequação da metodologia para o teste de germinação de sementes de pitaia vermelha

Alves, Charline Zaratin; Godoy, Amanda Regina; Corrêa, Luíz de Souza

doi:10.1590/s0103-84782011005000051

Cited by 14 publications

(12 citation statements)

References 13 publications

(7 reference statements)

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“…This time was higher than the time required in this study since the stabilization of the germination process was achieved in 9, 8, and 25 days at 20, 25, and 30 °C, respectively, after the start of the tests. Alves et al (2011) observed that in all substrates, germination ceased 10 days after sowing, which was similar to the results obtained in this study. The ideal temperature for the germination of a species tends to require less time for stabilization of the seed germination process, and thereby the germination is faster; this conclusion was corroborated by the SGI and MGT variables (Figure 1).…”

Section: Resultssupporting

confidence: 91%

“…It is worth mentioning that the germination rate of seeds grown on filter paper in the study by Andrade et al (2008) is considerably lower than that observed in this study (60% vs. 95 to 98%, respectively), whereas the maximum germination rate of seeds grown on a paper roll at 25 °C obtained by Alves et al (2011) was 85%.…”

Section: Resultscontrasting

confidence: 77%

“…Alves et al (2011), evaluated the paper, sand, vermiculite, and soil substrates under constant and alternating temperatures of 20, 25, and 30 °C and 20-30 °C, and observed that the paper substrate and a temperature of 25 °C were favorable conditions for the initial and final evaluation of germination rate of pitaya seeds. Andrade et al (2008) evaluated six substrates for the germination of the pitaya seed.…”

Section: Resultsmentioning

confidence: 99%

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Tests for evaluating the physiological quality of pitaya seeds

Ortiz¹,

Moritz²,

Takahashi³

et al. 2015

Sem. Ci. Agr.

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Germination test is used to assess the physiological quality of seeds; however, since it is carried out under ideal conditions, this test has not been shown sufficient for this purpose. Instead, it is possible to use vigor tests, although the lack of standardized methodologies has reduced their applicability and reproducibility. Thus, this study aimed to develop methodologies for conducting tests of germination, accelerated aging, and electrical conductivity for the evaluation of the physiological quality of pitaya seeds. For this purpose, seeds from ripe Hylocereus undatus fruits were used. A completely randomized experimental design was used with four replications. The physiological quality of the seeds was assessed using germination, accelerated aging, and electrical conductivity tests, and the speed of germination index (SGI) and mean germination time (MGT) were determined for both the germination test and accelerated aging test. For the statistical analysis, we performed regression model adjustments and calculated the Pearson correlation coefficient (p < 0.05). The germination test for H. undatus seeds can be performed at 25 °C, with the aim of reaching the highest SGI and lowest MGT values. The accelerated aging test can be conducted at 43 °C for 48 h, because combining these factors favors the expression of seed vigor, allowing seeds to achieve the maximum SGI and minimum MGT, while reducing the time of the assay. The electrical conductivity test can be performed using 25 seeds at a temperature of 30 °C and a water volume of 10 mL, since under these conditions there is less interference from external factors on the leachate content of the solution. Key words: Accelerated aging, cactaceae, electrical conductivity, germination, Hylocereus undatus, vigor ResumoO teste de germinação é utilizado na avaliação da qualidade fisiológica de sementes; porém, devido à sua condução em condições ideais, não tem se mostrado suficiente para este propósito. Nesse sentido, há a possibilidade de recorrer aos testes de vigor, conquanto, a ausência de metodologias padronizadas tem dificultado a sua aplicabilidade e reprodutibilidade. Assim, o trabalho teve como objetivo determinar as metodologias dos testes de germinação, envelhecimento acelerado e condutividade elétrica, a fim de avaliar a qualidade fisiológica de sementes de pitaia. Para o estudo foram utilizadas sementes de frutos maduros de Hylocereus undatus. O delineamento experimental foi o inteiramente casualizado, com quatro repetições. A qualidade fisiológica das sementes foi avaliada pelos testes de germinação, envelhecimento acelerado e condutividade elétrica, além da determinação do índice de velocidade de germinação (IVG) e do tempo médio de germinação (TMG), ambos, em conjunto

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Section: Resultssupporting

confidence: 91%

Section: Resultscontrasting

confidence: 77%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Tests for evaluating the physiological quality of pitaya seeds

Ortiz¹,

Moritz²,

Takahashi³

et al. 2015

Sem. Ci. Agr.

View full text Add to dashboard Cite

show abstract

“…Seeds of different species have shown unique reaction to temperature, which is optimal when they express the maximum potential of germination in the least period (Borges and Rena, 1993;Popinigis, 1985). Some species respond to alternating temperatures, while others best germinate under constant temperature (Alves et al, 2011). The substrate (physical support in which the seed is placed) functions at maintaining ideal conditions for germination and emergence.…”

Section: Introductionmentioning

confidence: 99%

Standardizing germination tests for quinoa seeds

Souza¹,

Souza²,

Souza³

et al. 2017

Afr. J. Agric. Res.

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The standard germination tests have been commonly used on commercial grain crops, such as soybean, field beans, rice and maize. However, there are no standard tests for potential new crops; quinoa being one of them. This work is aimed at evaluating the effect of substrate, temperature and counting time in seed germination of quinoa. The following treatments were used in tests with seeds: on blotter paper, between blotter paper, on sand and within sand, all previously soaked with distilled water. These substrates were combined to the temperatures of 15, 20, 25, 30°C, and alternated between 20 to 30°C. The experiment was conducted on an entirely randomized factorial design 5 × 4 (temperature × substrate) with 4 repetitions. Normal seedlings, abnormal seedlings and dead seeds were counted until stabilized. The germination velocity index and mean time for germination were calculated. From the results, it was concluded that germination test of quinoa seeds should be conducted at 20 to 30°C alternate temperatures, on or between blotter paper, with initial count at 2 days and final count at 4 days.

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“…Para a maioria das espécies tropicais e algumas espécies de Passiflora, a temperatura ótima de germinação encontra-se entre a faixa de 15 a 30 °C (ALVES et al 2011) Comprimento de plântulas (COMP), biomassa seca de plântulas (BMSP), percentagem de germinação (PG), índice de velocidade de germinação (IVG) e tempo médio de germinação (TMG) de sementes de granadilla, submetidas as temperaturas.…”

Section: Resultsunclassified