Desenvolvimento de populações duplo-haplóides de cevada cervejeira associadas à atividade das enzimas (1-3, 1-4)-β-glucanases

Abstract

RESUMO Populações duplo-haplóides apresentam especial vantagem para análises genéticas, uma vez que a informação ABSTRACT Doubled haploid populations offer special advantages in genetic analyses, since the information they provide may be maximized due to the fact that all loci are homozygous. The aim of this study was to develop two barley (Hordeum vulgare ssp.vulgare L.) doubled-haploid (DHs) populations segregating to (1-3, 1-4)--glucanases activity, utilizing the anther culture protocol. Two crosses were performed with contrasting cultivars to (1-3, 1-4)--glucanases activity. Parental cultivars used were 'MN 698' and 'CEV 97047' for the development of 'green malt' population (MV) and Embrapa 127 and 'CEV 96025' for the development of "dry malt" population (MS). For the MS and MV populations, 10,734 and 4,139 anthers were cultured, respectively