An antithrombin-dependent sulfated polysaccharide isolated from the green alga Caulerpa cupressoides has in vivo anti- and prothrombotic effects

Rodrigues, José Ariévilo Gurgel; Queiroz, Ismael Nilo Lino de; Quinderé, Ana Luíza Gomes; Vairo, Bruno C.; Mourão, Paulo Antônio de Souza; Benevídes, Norma Maria Barros

doi:10.1590/s0103-84782011000400014

Cited by 40 publications

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“…Total SPs (TSPs) were obtained through the methodology previously described by Rodrigues et al (2010c). Initially, dried and milled seaweed (25°C, 5 g) were rehydrated with 250 mL of 100 mM sodium acetate buffer (AcNa, pH 5.0) containing cysteine and EDTA (5 mM).…”

Section: Sps Extractionmentioning

confidence: 99%

“…After precipitation, TSPs were centrifuged, subjected to two washes with 100 mL of commercial ethanol at 80%, and a third wash with ethanol of commercial degree (100 mL). Finally, the TSPs were dried in an air circulation oven (60°C, 4h) (RODRIGUES et al, 2010a).…”

Section: Sps Extractionmentioning

confidence: 99%

“…They also provide byproducts for several applications in Biotechnology (ARAÚJO et al, 2008;AMORIM et al, 2012;CAMPO et al, 2009;CARVALHO et al, 2009;GHOSH et al, 2004;RODRIGUES et al, 2010a;SOUZA et al, 2008;VANDERLEI et al, 2010;ZHANG et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

“…Probably these compounds in algae are related to mechanical, ionic and osmotic regulation, favoring the survival of these organisms in the marine environment (KLOAREG; QUATRANO, 1988). The environmental conditions and the study on different species of seaweeds provide a great structural variety of their polysaccharides, leading to particular pharmacological implications, when these compounds are used in several models of biological activities, in prospect of discovering new drugs (ASSREUY et al, 2008;FONSECA et al, 2008;GHOSH et al, 2004;JI et al, 2008;PEREIRA et al, 2005;RODRIGUES et al, 2010b;SILVA et al, 2010;ZHANG et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

“…Green marine algae of the genus Caulerpa Lamouroux (1809) are commonly found on the Brazilian coast (RODRIGUES et al, 2010c;RODRIGUES et al, 2011a) and are characterized by filamentous coenocytic organization, with a creeping stem of macroscopic shape, attached to substrate by a rhizomatous portion. According to TRI (2009), it has been reported that Caulerpa is an important contributor to the algal biomass of coral reefs and laggons.…”

Section: Introductionmentioning

confidence: 99%

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Comparative study of sulfated polysaccharides from Caulerpa spp. (Chlorophyceae). Biotechnological tool for species identification?

Rodrigues

Quinderé

Queiroz

et al. 2012

Acta Sci. Biol. Sci.

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ABSTRACT. Studies on macromolecules isolated from marine algae suggested sulfated polysaccharides (SPs) as possible molecular markers for species. We evaluated isolated and fractionated SPs from the green marine algae Caulerpa cupressoides, C. prolifera and C. racemosa collected at Pacheco Beach, as possible taxonomic molecular indicators. Total SPs were extracted with papain in 100 mM sodium acetate buffer (pH 5.0) containing cysteine and EDTA (both 5 mM), followed by ion-exchange chromatography on DEAE-cellulose using a NaCl gradient. The obtained fractions were analyzed by 0.5% agarose gel electrophoresis. Anticoagulant assays employing normal human plasma and standard heparin ) by the activated partial thromboplastin time (APTT) test were also performed as comparison parameters. Low yields, and similar chromatographic profiles were found among species' SPs, but electrophoresis revealed distinct SPs resolution patterns. The changes in APTT of SP fractions were dependent on charge density as showed by electrophoresis profiles. Activities were 17.37 (C. cupressoides), 22.17 (C. racemosa) and 25.64 (C. prolifera) IU mg -1 , respectively, similar to a previous study using the first and second species. The results suggest that comparative studies of SPs isolated from seaweeds may be an important tool for the identification of Caulerpaceae.Keywords: chlorophyta, sulfated polysaccharides, molecular markers, APTT test.Estudo comparativo dos polissacarídeos sulfatados de clorofíceas Caulerpa spp. (Chlorophyceae). Ferramenta biotecnológica na identificação de espécies? RESUMO. A utilização de macromoléculas isoladas de organismos marinhos sugere correlacionar características em estudos taxonômicos e a investigação comparativa de polissacarídeos sulfatados (PSs) de algas despertam seu interesse como marcadores moleculares. Objetivou-se avaliar PSs isolados e fracionados das algas marinhas verdes Caulerpa cupressoides, C. prolifera e C. racemosa, coletadas na Praia do Pacheco, Estado do Ceará, como possíveis indicadores moleculares taxonômicos. Os PSs totais foram extraídos com papaína em tampão acetato de sódio 100 mM (pH 5,0) contendo cisteína e EDTA (ambos 5 mM), seguido por cromatografia de troca iônica em coluna de DEAE-celulose utilizando um gradiente de NaCl. As frações obtidas foram analisadas por eletroforese em gel de agarose a 0,5%. Ensaios anticoagulantes, utilizando o teste do tempo de tromboplastina parcial ativada (TTPA) com plasma humano normal e heparina padrão ), também foram realizados como parâmetros de comparação. Verificaram-se baixos rendimentos e semelhantes perfis cromatográficos entre os PSs das espécies, porém revelando, por eletroforese, diferenças moleculares marcantes. As alterações no TTPA das frações de PS foram dependentes da densidade de cargas negativas mostradas nos perfis eletroforéticos, cujas atividades foram 17,37 (C. cupressoides), 22,17 (C. racemosa) e 25,64 (C. prolifera) UI mg -1 , respectivamente, e tal propriedade justificou um estudo já realizado utilizando a primeira e se...

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Section: Sps Extractionmentioning

confidence: 99%

Section: Sps Extractionmentioning

confidence: 99%