Antibody responses in mice after immunization with inactivated bovine respiratory syncytial virus using different adjuvants

Spilki, Fernando Rosado; Almeida, Renata Servan de; Arns, Clarice Weis

doi:10.1590/s0103-84782010005000188

Cited by 2 publications

(1 citation statement)

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“…As possíveis consequências práticas deste fato, em especial para a proϐilaxia da infecção, já que todas as vacinas comercializadas no país trazem vírus do subgrupo A, devem ser estudadas com experimentos controlados em bovinos ou em modelos animais (Duncan Jr et al 1993, Schrijver 1998, Spilki & Arns 2010.…”

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Detecção molecular e análise filogenética de vírus respiratório sincicial bovino (BRSV) em swabs e tecido pulmonar de bovinos adultos

2011

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Bovine respiratory syncytial viruses virus (BRSV) is one of the etiologic agents of pneumonia in young cattle. Few studies have been made aiming detection of the virus in samples collected from adult animals, especially those asymptomatic bovines. However, it is assumed that infections in these groups may occur mostly asymptomatic and this would be an important mechanism for maintaining of BRSV in herds. In this study, the goal was to conduct an analysis of the occurrence of asymptomatic infections by BRSV in lung samples (n=68) and nasal swabs (209) taken from adult animals collected in abattoirs from Southern and Southeastern Brazil respectively, to detect via polymerase chain reaction the occurrence of infected animals in populations of adult cattle. The samples that resulted positive (6) on RT-PCR were subsequently subjected to cutting with restriction enzymes and sequencing for genetic characterization (2 samples). All samples belongs to subgroup B of BRSV, which is reported as the one circulating in Brazil. The results obtained demonstrate that BRSV may be present in samples taken from adult animals, which is in agreement the hypothesis that infections in adults run in a sub-clinical way that may be of importance as a maintenance mechanism of the virus in bovine herds. RESUMO.-O vírus respiratório sincicial bovino (BRSV) é um dos agentes etiológicos de pneumonias em bovinos jovens. Poucos estudos foram realizados visando à detecção do agente em amostras coletadas de animais adultos, e em especial de bovinos assintomáticos. No entanto, presume--se que as infecções ocorridas nestes grupos possam ocorrer em sua maioria de forma assintomática e este seria um mecanismo importante para manutenção do BRSV nos rebanhos. No presente estudo, o objetivo foi realizar uma análise da prevalência de infecções assintomáticas pelo BRSV em pulmões (n=68) e swabs nasais (209) coletados de bovinos adultos coletadas em frigoríϐicos da região Sul e Sudeste respectivamente, no sentido de detectar por intermédio de reação da polimerase em cadeia qual a taxa de animais infectados em populações de animais adultos onde não ocorram sinais clínicos da infecção. As amostras positivas à RT-PCR (6) foram posteriormente submetidas ao corte com enzimas de restrição (REA) e sequenciamento para caracterização genética do gene F (2 das amostras). Todas as amostras se enquadram no subgrupo B de BRSV, o grupo circulante no Brasil conforme estudos anteriores. Os resultados obtidos demonstram que o BRSV pode estar presente em amostras obtidas de animais sadios, reforçando a hipótese de que infecções subclínicas

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Section: Discussionunclassified

Detecção molecular e análise filogenética de vírus respiratório sincicial bovino (BRSV) em swabs e tecido pulmonar de bovinos adultos

2011

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Immunoadjuvant and Anti-Inflammatory Plant Saponins: Characteristics and Biotechnological Approaches Towards Sustainable Production

Costa

Yendo²,

Fleck³

et al. 2011

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Saponins can be classified as triterpenoid (C30) or steroidal (C27), based on their carbon nucleus (aglycone). Sugar residues are linked to the aglycone, conferring an amphiphilic nature on these molecules, which is relevant for their biological activities. Saponins include a large variety of molecules that find several applications in pharmacology. Saponins have been shown to display immunoadjuvant, anti-inflammatory, antiplatelet, hypocholesterolemic, antitumoral, anti-HIV, antibacterial, insecticide, fungicide and anti-leishmanial activities. Anti-inflammatory medicines are increasingly demanded to treat various forms of arthritis in aging and obese populations and to help reduce the doses and duration of conventional corticotherapy with less side effects and without immunosuppression. The vaccine market for both human and veterinary uses is close to US$ 15 billion, progressively inflated by the recurrent threat of global pandemics.This paper provides an overview of recent advances (main focus on the last five years) on plant saponins that show anti-inflammatory and/or immunoadjuvant activities: source plants, isolation procedures, mechanism of action and biotechnological approaches towards sustainable production of bioactive saponins. Special attention is given to ginseng and Quillaja saponins. Strategies based on plant cultivation, cell and tissue culture, elicitation, and metabolic engineering for improved production of saponins are described. Future directions for research in the field and strategies to overcome bottlenecks are also discussed.

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Antibody responses in mice after immunization with inactivated bovine respiratory syncytial virus using different adjuvants

Cited by 2 publications

References 20 publications

Detecção molecular e análise filogenética de vírus respiratório sincicial bovino (BRSV) em swabs e tecido pulmonar de bovinos adultos

Detecção molecular e análise filogenética de vírus respiratório sincicial bovino (BRSV) em swabs e tecido pulmonar de bovinos adultos

Immunoadjuvant and Anti-Inflammatory Plant Saponins: Characteristics and Biotechnological Approaches Towards Sustainable Production

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