Crescimento e composição química de dez espécies de microalgas marinhas em cultivos estanques

Borges-Campos, Viviane; Barbarino, Elisabete; Lourenço, Sergio de Oliveira

doi:10.1590/s0103-84782010005000009

Cited by 11 publications

(7 citation statements)

References 24 publications

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“…All recent applications on microalgae are favored by high productivity and a precious chemical profile of the cultivated species. However, the chemical content of microalgae varies widely due to differences with culture density, age, changes in culture conditions, methods of measurement used, and the physiological state of the microalgae and to the growth phase of the culture [ 10 , 11 ]. Continuous and semi-continuous cultures have gained attention not only for providing a constant source of microalgal biomass, but also for permitting precise manipulation of their biochemical composition [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Molecular identification, biomass, and biochemical composition of the marine chlorophyte Chlorella sp. MF1 isolated from Suez Bay

El‐Sheekh

Abu-Faddan

Abo-Shady

et al. 2020

Journal of Genetic Engineering and Biotechnology

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Background An Egyptian indigenous unicellular green microalga was isolated from the coastal water of Suez Bay (N 29.92°, E 32.473°), Red Sea, Egypt. The molecular analysis based on 18S rRNA sequence showed that the gene sequence for this strain was highly similar (100% identity and 98% query cover) to different Chlorella strains isolated from different habitats. Results The observed morphological characters together with the molecular phylogeny assigned the isolated microalga as Chlorella sp. MF1 with accession number KX228798. This isolated strain was cultivated for estimation of its growth and biochemical composition. The mean specific growth rate ( μ ) was 0.273 day −1 . Both the biomass productivity and the cellular lipid content increased by increasing salinity of the growth medium, recording a maximum of 6.53 g DW l −1 and 20.17%, respectively, at salinity 40.4. Fourteen fatty acids were identified. The total saturated fatty acid percentage was 54.73% with stearic (C18:0), arachidic (C20:0), and palmitic acids (C16:0) as major components, while the total unsaturated fatty acid percentage was 45.27% with linoleic acid (C18:2c) and oleic acid (C18:1) as majors. Conclusion This algal strain proved to be a potential newly introduced microalga as one of the most proper options available for microalgae-based biodiesel production. The proximate analysis showed the protein content at 39.85% and carbohydrate at 23.7%, indicating its accessibility to various purposes.

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Section: Introductionmentioning

confidence: 99%

Molecular identification, biomass, and biochemical composition of the marine chlorophyte Chlorella sp. MF1 isolated from Suez Bay

El‐Sheekh

Abu-Faddan

Abo-Shady

et al. 2020

Journal of Genetic Engineering and Biotechnology

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“…According to Borges-Campos et al (2010) microalgal species with small cell volumes tend to have higher growth rates in comparison to large-sized cells. This interpretation seems to be coherent here, explaining well the high growth rate of S. subsalsus, a small-sized species.…”

Section: Growth Cell Biovolumes and Nutrient Uptakementioning

confidence: 99%

An assessment of the usefulness of the cyanobacterium Synechococcus subsalsus as a source of biomass for biofuel production

Setta¹,

Barbarino²,

Passos³

et al. 2014

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ABSTRACT. Nowadays algal biofuels are considered one of the most promising solutions of global energy crisis and climate change for the years to come. By manipulation of the culture conditions, many algal species can be induced to accumulate high concentrations of particular biomolecules and can be directed to the desired output for each fuel. In this context, the present study involved the assessment of the effects of CO 2 availability and nitrogen starvation on growth and chemical composition of the cyanobacterium Synechococcus subsalsus, testing a fast-growing native strain. The control experiments were performed with Conway culture medium in 12-day batch cultures, in 6-liter flasks and 12 h photoperiod, with addition of 2 L min -1 filtered air to each flask. Other two experimental conditions were also tested: (i) the placement into the cultures of additional dissolved nutrients except nitrogen, one week after the start of growth (N-), and (ii) the input of pure CO 2 into the flasks from the 5 th day of growth (C+). In all cultures, daily cell counts were done throughout the cultivation, as well as measurements of pH and cell biovolumes. Maximum cell yield were found in N-experiments, while cell yields of C+ and control were similar. Dissolved nitrogen was exhausted before the end of the experiments, but dissolved phosphorus was not totally consumed. Protein and chlorophyll-a concentrations decreased from the exponential to the stationary growth phase of all experiments, except for protein in the control. In all experiments, carbohydrate, lipid and total carotenoid increased from the exponential to the stationary growth phase, as an effect of nitrogen limitation. Increments in carbohydrate concentrations were remarkable, achieving more than 42% of the dry weight (dw), but concentrations of lipid were always lower than 13% dw. The addition of pure CO 2 did not cause a significant increase in biomass of S. subsalsus nor generated more lipid and carbohydrate than the other treatments. Nitrogen starvation caused an intense accumulation of carbohydrate, but the increments of lipid were small. Despite the fast growth, the cyanobacterium S. subsalsus has a virtually null potential for biodiesel production, given its low lipid concentrations. The high concentrations of carbohydrate combined with fast growth point to the potential use of this species as raw material for other possible biotechnological processes, after a demonstration of technical and economic viability. Keywords: biodiesel, biomass, cultivation, lipid, microalgae, cyanobacteria, productivity.Evaluación de la utilidad de la cianobacteria marina Synechococcus subsalsus como fuente de biomasa para la producción de biocombustibles RESUMEN. Actualmente los biocombustibles elaborados con algas se consideran una de las soluciones más prometedoras de la crisis energética mundial y el cambio climático para los años venideros. Mediante la manipulación de las condiciones de cultivo, muchas especies de algas pueden ser inducidas para acumular altas concentracione...

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“…Sequences for the 18S rRNA gene were used to identify several microalgal species and in particular to differentiate species of Chlorella [12,13]. In recent years, microalgae achieve high potential as a feedstock for biofuel production due to their several advantages such as higher biomass productivity, lesser water demand, and no agricultural land requirement compared to other energy crops [14][15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Standardization and Application of PCR Targeting Chlorella Species Isolated From Environmental Samples

Premina¹,

Andrew²,

Sundaralingam³

et al. 2021

Int J Pharm Pharm Sci

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Objective: Identification of Chlorella species from the environment through 18s ribosomal RNA sequencing. This study was aimed to design primer targeting Chlorella and other closely related algal species targeting 18s ribosomal RNA, ITS1 region. Methods: Sanger sequencing was carried out for the identification of algae up to the genus and species level using an in-house designed primer and optimized PCR conditions. Results: Out of 2 algae samples identified phenotypically, one isolate identified as Chlorella vulgaris and other one identified as Chlorella sorokiniana based on the results of Basic Alignment Search Tool (BLAST). Conclusion: To conclude, this study provided primers with PCR conditions to characterize algal samples through molecular identification with 100% accuracy than the phenotypic method.

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Crescimento e composição química de dez espécies de microalgas marinhas em cultivos estanques

Cited by 11 publications

References 24 publications

Molecular identification, biomass, and biochemical composition of the marine chlorophyte Chlorella sp. MF1 isolated from Suez Bay

Molecular identification, biomass, and biochemical composition of the marine chlorophyte Chlorella sp. MF1 isolated from Suez Bay

An assessment of the usefulness of the cyanobacterium Synechococcus subsalsus as a source of biomass for biofuel production

Standardization and Application of PCR Targeting Chlorella Species Isolated From Environmental Samples

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