2010
DOI: 10.1590/s0103-84782010001100027
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Técnicas de coloração para detecção de Encephalitozoon cuniculi em cortes histológicos

Abstract: RESUMO No presente trabalho, foram avaliadas diferentes técnicas de coloração aplicadas a cortes histológicos para a identificação de

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Cited by 4 publications
(3 citation statements)
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“…22 In fact, Gram stain has not only been used in mammalian tissues, but also to detect microsporidian spores in zebrafish (Danio rerio). 45,46 In our study, color did not vary between immature (red) and mature (purple) spores, as reported by others, 28,61 and the internal details of E. cuniculi spores, such as the belt-like stripe, were not observed. MaT stain is routinely used for demonstrating connective tissue.…”
Section: Discussionsupporting
confidence: 86%
See 1 more Smart Citation
“…22 In fact, Gram stain has not only been used in mammalian tissues, but also to detect microsporidian spores in zebrafish (Danio rerio). 45,46 In our study, color did not vary between immature (red) and mature (purple) spores, as reported by others, 28,61 and the internal details of E. cuniculi spores, such as the belt-like stripe, were not observed. MaT stain is routinely used for demonstrating connective tissue.…”
Section: Discussionsupporting
confidence: 86%
“…50 Our results contrast with another study using immunosuppressed BALB/c mice, where Giemsa stain was suitable for detecting E. cuniculi spores. 28,55 Grocott stain detects glycogen, mucin, and argyrophilic components in fungi. 46 This stain has been found to be useful for the detection of microsporidian spores in human ocular tissue.…”
Section: Discussionmentioning
confidence: 99%
“…Enterococcus faecalis foi a bactéria escolhida no presente estudo pois, é uma bactéria frequentemente encontrada em casos de retratamento e lesões primárias, pois apresenta capacidade de penetração nos túbulos dentinários, podendo permanecer viáveis em seu interior, além de possuir resistência a algumas substâncias antimicrobianas. (9,24,32,42) No presente estudo, foi realizada a contaminação intratubular dos espécimes pelo protocolo de Andrade et al (2015), (2) que tem sido utilizado por muitos estudos. (2,15,18) O modelo experimental foi escolhido a fim de simular a situação endodôntica real, onde essa infecção ocorre em profundidade nos túbulos dentinários.…”
Section: Referênciasunclassified