Isolamento, fracionamento e atividade anticoagulante de iota-carragenanas da Solieria filiformis

Abstract

RESUMO Este estudo teve como objetivo isolar, fracionar e avaliar o potencial anticoagulante de iota-carragenanas (iCARs) da rodofícea Solieria filiformis, quando obtidas por dois métodos de extração (M I e M II). As i-CARs foram isoladas ABSTRACT This study aimed to isolate, fractionate and evaluate the anticoagulant potential of iota-carrageenans (i-CARs) from Solieria filiformis when two extraction methods (M I and M II) were used. i-CARs were isolated with papain in 0.1M sodium acetate (pH 5.0) containing 5mM cystein and 5mM EDTA (M I) or water (80°C) (M II), and then their chemical composition of total carbohydrates, free sulfate (FS) and contaminant proteins were determined. i-CARs were submitted to anionexchange chromatography (DEAE-cellulose) using a sodium chloride gradient,being evaluated the activated partial thromboplastin time (APTT) and prothrombin time of obtained fractions and compared to heparin (

Iota-carrageenans from Solieria filiformis (Rhodophyta) and their effects in the inflammation and coagulation

“…As can be noted, the fractions had no practical effects in the normal coagulation time (Table 3) for EE, RHWE and HWE, respectively), but was less potent than the standard HEP ). These data are indicative that ι-CARs fractions from S. filiformis discretely act on the intrinsic and/or common pathways of the blood coagulation system (Table 3), but without effects on the prothrombin test (data not shown) (RODRIGUES et al, 2010b). Similar results were also found for Caulerpa SPs of green seaweeds recently investigated by Rodrigues et al (2010a).…”

“…In contrast, the lowest lyophilized yield of CARs in this experiment was 19.14 ± 0.48% (EE), whereas 46.8 and 25% of crude extracts of ι-CARs (EE) of S. filiformis were isolated by Pontes et al (2009) and Assreuy et al (2010), respectively, although dried overnight (60°C; 24h). No CPs was detected by the employment of papain digestion also in comparison to others studies (RODRIGUES et al, 2010b;RODRIGUES et al, 2011). These results suggest a selectivity of EE for obtaining of seaweeds carbohydrates.…”

“…Our research group evaluated the employment of different extraction methods for obtaining SPs from seaweeds (PEREIRA et al, 2005;RODRIGUES et al, 2010b;RODRIGUES et al, 2011). In the present study, the S. filiformis ι-CARs yield was different among the extraction methods used (Table 1).…”

“…The ι-CARs obtained by all methods were centrifuged, redissolved in distilled water, dialyzed against distilled water for 24h (47 × 27 mm cellulose membrane, Sigma-Aldrich, St. Louis, MO, USA), and then freeze-dried (RODRIGUES et al, 2010b). The ι-CARs yields (%) were calculated as the percentage of dry matter.…”

Isolamento, fracionamento e avaliação toxicológica in vivo de polissacarídeos sulfatados de Hypnea musciformis

“…As diversas propriedades desses compostos têm motivado a maricultura de algas no Brasil, como, por exemplo, as espécies rodofíceas Hypnea musciformis (REIS et al, 2006) e Solieria filiformis (RODRIGUES et al, 2010), além de propiciar estudos relacionados à biologia de algas, extração e caracterização físico-química e de atividades biológicas de ficocolóides (RODRIGUES et al, 2010;RODRIGUES et al, 2011b).…”