The aims of this work were to investigate the effects of gamma irradiation on population, viability and aflatoxin B production of strains isolated from nutmeg kernels. Thirteen strains of. were isolated and cultured in potato dextrose agar. Conidia were harvested, air-dried, and irradiated 0, 5, or 10 kGy with gamma rays using a Cobalt-60 source. Toxigenicity were determined using a coconut agar medium and thin layer chromatography. Genomes of strains were extracted from mycelia. Four sets of primers, regulatory () and structural genes (-, -, -) were used to confirm the presence of the genes. Our results indicate that total fungal populations decreased significantly ( < 0.05) with increasing irradiation dose. However, doses of 5 and 10 kGy were insufficient to completely eliminate the viability of some . strains. Irradiation did not change toxigenicity and triggered surviving toxigenic strains to produce aflatoxin B.