Immunohistochemical expression of B and T-lymphocytes and TGF-β in experimentally transplanted canine venereal tumor

Silveira, Ana Carolina Trompieri; Gerardi, Daniel Guimarães; Mouro, Juliana Vitti; Costa, Mirela Tinucci; Alessi, Antônio Carlos

doi:10.1590/s0103-84782009005000025

Cited by 6 publications

(2 citation statements)

References 15 publications

(24 reference statements)

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“…Further, the stimulation of a particular lymphocyte subtype may depend on the oncolytic or therapeutic agent being used. Much lower CD4 + and CD8 + counts have been reported during spontaneous regression of untreated, experimentally-induced TVT cases 23 .…”

Section: Resultsmentioning

confidence: 94%

Canine Parvovirus ns1 gene and Chicken Anemia vp3 gene induce partial oncolysis of Canine Transmissible Venereal Tumor

Bhat

Ganguly

Tiwari

et al. 2017

Sci Rep

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The oncolytic effect of Canine Parvovirus ns1 gene and Chicken Anemia vp3 gene in naturally occurring cases of Canine Transmissible Venereal Tumor (CTVT) is being reported. Dogs suffering from CTVT (N = 18) were systematically randomized into three groups viz. A, B, and C (n = 6). Animals of the groups A, B, and C received 100 µg of the ns1 gene, vp3 gene, and ns1 + vp3 gene combination, respectively, for three weeks intratumorally at weekly intervals; results were normalized against base values before commencement of therapy and after complete remission that were taken as negative and positive controls, respectively. Initiation of oncolytic gene therapy arrested the further progression of the tumor but most of the animals in the study underwent incomplete remission, indicating incomplete activity of ns1 and vp3 genes. The oncolytic effect of the treatments was in the order ns1 > vp3 > ns1 + vp3. Oncolysis was accompanied by decreased mitotic index and AgNOR count, and increased TUNEL positive cells and CD4+ lymphocyte counts. Our findings show that Canine Parvovirus ns1 may eventually find an important role as an oncolytic agent.Oncolytic viruses replicate preferentially in cancer cells and kill them at the end of the replication cycle. Live viruses were first used for the treatment of cancer more than a century ago. However, soon after its inception, the approach had to be abandoned due to problems of toxicity, development of antiviral immunity and risk of re-emergence of virulent viruses 1 . Lately, there has been a revival of scientific interest in the development of virus-based cancer therapeutics. Molecular biotechnology has allowed genetic engineering of viruses for enhanced selectivity towards tumor cells -an approach that was first demonstrated with Herpes Simplex virus type-1 (HSV-1) in an experimental glioma model 2 . Successively, several viruses with high oncolytic capacity have been identified as potential candidates for clinical applications. Chicken anemia virus and canine parvovirus are two such viruses that possess inherent oncolytic properties 3 . The chicken anemia viral protein (VP) 3, also termed apoptin, specifically, kills tumor cells while sparing normal cells. Upon expression in normal cells, apoptin is accumulated in the cytoplasm whereas in cancer cells, it is specifically targeted to the nucleus where it elicits its lethal effects 4 . Canine parvovirus-2 (CPV-2) is an extremely simple DNA virus that encodes one to two early products and a limited number of late structural proteins. It lacks mechanisms for inducing S phase and replicates only in proliferating host cells. This feature confers additional selectivity for killing rapidly growing cancer cells. The apoptosis-inducing activity of parvoviruses was mapped to the non-structural protein-1,-2 (ns1/ns2) of minute virus of mice (MVM) and ns1 of parvovirus B19 5 . Unlike apoptin, ns1 does not rely solely on apoptosis for its oncolytic effect.Biological similarities with humans make the dog an ideal comparative model system for study...

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Section: Resultsmentioning

confidence: 94%

Canine Parvovirus ns1 gene and Chicken Anemia vp3 gene induce partial oncolysis of Canine Transmissible Venereal Tumor

Bhat

Ganguly

Tiwari

et al. 2017

Sci Rep

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“…However other tumors cases have also been reported like perianal gland adenoma (Chaudhari et al, 2017) [4] . TVT is naturally contagious and sexually transmissible among dogs, particularly stray dogs and dogs of reproductive age (Tinucci-Costa & Castro, 2009) [51] , and experimental transplantation has also been proved to be a possibility (Silveira et al, 2009) [48] . Although sexual actions are the most common method of TVT transmission, other methods exist, such as the habit of licking and sniffing other canines (Oliveira, 2019) [31] , which can result in tumor cell implantation in the nasal and oral canals.…”

Section: Transmissible Venereal Tumors (Tvt)mentioning

confidence: 99%

Review on important diseases of Dogs: At glance

Solanki¹,

Desai²,

Dalvi³

et al. 2023

Int. J. Vet. Sci. Anim. Husbandry

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Dogs are one of the most accepted pet animals in every household. Increasing urbanization and modernization of human life has created a space where people are living in nuclear family or alone. This has created peoples' perception and acceptance of having pet for companionship. Humandog interaction and close livelihood confinement emerge as crucial threat of outbreaks of zoonotic disease even a dog's diseases. To prevent and control diseases of dogs, it requires proper knowledge of vaccination, education of pet health management and pets' diseases. Therefore, the current review is on describing dog diseases like, Rabies, Canine Parvoviral gastroenteritis, Canine Distemper, Canine Coronaviral infection, Canine Rotavirus infection, Canine Herpesvirus infection, Canine Leptospirosis, Canine Brucellosis, Transmissible venereal tumors (TVT), Kennel cough, Pyoderma at glance which provides concise information regarding the causative agents and other relevant details.

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Cell-mediated immunity and expression of MHC class I and class II molecules in dogs naturally infected by canine transmissible venereal tumor: Is there complete spontaneous regression outside the experimental CTVT?

Duzanski

Flórez

Fêo

et al. 2022

Research in Veterinary Science

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Immunohistochemical expression of B and T-lymphocytes and TGF-β in experimentally transplanted canine venereal tumor

Cited by 6 publications

References 15 publications

Canine Parvovirus ns1 gene and Chicken Anemia vp3 gene induce partial oncolysis of Canine Transmissible Venereal Tumor

Canine Parvovirus ns1 gene and Chicken Anemia vp3 gene induce partial oncolysis of Canine Transmissible Venereal Tumor

Review on important diseases of Dogs: At glance

Cell-mediated immunity and expression of MHC class I and class II molecules in dogs naturally infected by canine transmissible venereal tumor: Is there complete spontaneous regression outside the experimental CTVT?

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