Protein and antigen profiles of Leptospira interrogans serovar Hardjo

Lafetá, Bárbara Nobre; Castro, Elaine Cristina de; Silva, Nivaldo da

doi:10.1590/s0103-84782009000900024

Cited by 1 publication

(1 citation statement)

References 13 publications

(13 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among the proteins strongly reacting with the tested animal and human sera, a protein with a mass of 25 kDa was found in those reports. This protein has It is worth mentioning that there are several reports that address the identification of leptospiral proteins (i.e., leptospiral antigens) recognized by the host's humoral immune response during infection [34][35][36][37]. For this purpose, Western blotting analysis was also used in these studies.…”

Section: Immunogenic Potential Of Lic_10559mentioning

confidence: 99%

Immunoreactivity of a Putative ECF σ Factor, LIC_10559, from Leptospira interrogans with Sera from Leptospira-Infected Animals

Kędzierska‐Mieszkowska

Arent

2023

Pathogens

View full text Add to dashboard Cite

L. interrogans belongs to highly invasive spirochaetes causing leptospirosis in mammals, including humans. During infection, this pathogen is exposed to various stressors, and therefore, it must reprogram its gene expression to survive in the host and establish infection in a short duration of time. Host adaptation is possible thanks to molecular responses where appropriate regulators and signal transduction systems participate. Among the bacterial regulators, there are σ factors, including ECF (extracytoplasmic function) σ factors. The L. interrogans genome encodes 11 putative ECF σE-type factors. Currently, none of them has been characterized biochemically, and their functions are still unknown. One of them, LIC_10559, is the most likely to be active during infection because it is only found in the highly pathogenic Leptospira. The aim of this study was to achieve LIC_10559 overexpression to answer the question whether it may be a target of the humoral immune response during leptospiral infections. The immunoreactivity of the recombinant LIC_10559 was evaluated by SDS-PAGE, ECL Western blotting and ELISA assay using sera collected from Leptospira-infected animals and uninfected healthy controls. We found that LIC_10559 was recognized by IgG antibodies from the sera of infected animals and is, therefore, able to induce the host’s immune response to pathogenic Leptospira. This result suggests the involvement of LIC_10559 in the pathogenesis of leptospirosis.

show abstract

Section: Immunogenic Potential Of Lic_10559mentioning

confidence: 99%