Isolamento de espécies enterobacterianas em Stomoxys calcitrans

Castro, Bruno Gomes de; Souza, Miliane Moreira Soares de; Bittencourt, Avelino José

doi:10.1590/s0103-84782008005000024

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References 19 publications

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“…The stable fly normally uses substrates with high potential to shelter bacterial agents with pathogenic capacity (CASTRO et al, 2008). Therefore, further studies on this fly are important to shed light on the impact of parasitism on livestock and public health.…”

Section: Resultsmentioning

confidence: 99%

Occurrence of Shiga-toxigenic Escherichia coli in Stomoxys calcitrans (Diptera: Muscidae)

Castro

Souza

Régua-Mangia

et al. 2013

Rev. Bras. Parasitol. Vet.

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This study aimed to verify the occurrence of Shiga toxin-producing Escherichia coli (STEC) strains in three distinct anatomic parts of the stable fly Stomoxys calcitrans by multiplex polymerase chain reaction (PCR Multiplex). According to the results obtained, E. coli was identified in 19.5% of the stable flies. Shiga toxin genes were detected in 13% of the E. coli isolated, most frequently from the surface, followed by abdominal digestive tract and mouth apparatus of insects, respectively. This is the first study to detect presence of STEC in Stomoxys calcitrans in Brazil; it has also revealed the potential role of stable flies as carriers of pathogenic bacterial agents.Keywords: Stomoxys calcitrans, Shiga toxin-producing Escherichia coli, Multiplex-PCR. ResumoEste estudo teve por objetivo avaliar a ocorrência de Escherichia coli Shiga-Toxigênica (STEC) em três diferentes partes anatômicas da mosca dos estábulos pela Reação de Polimerase em Cadeia Multiplex (PCR Multiplex). De acordo com os resultados obtidos, foi identificada E. coli em 19,5% das moscas dos estábulos colhidas. Foram detectados genes de produção de Shiga toxina em 13,63% das Escherichia coli isoladas, sendo mais frequente a superfície externa, seguido pelo trato digestivo abdominal e pelo aparelho bucal, respectivamente. Este foi o primeiro estudo no Brasil que detectou a presença de STEC em Stomoxys calcitrans e revelou o potencial papel da mosca dos estábulos em carrear um agente bacteriano patogênico.

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Section: Resultsmentioning

confidence: 99%