Enteritis associated with Lawsonia intracellularis and porcine circovirus type 2 infection in wild boars in Southern Brazil

Zlotowski, Priscila; Corrêa, Ana Grasielle Dionísio; Borba, Mauro Riegert; Cruz, Cláudio Estêvão Farias da; Driemeier, David

doi:10.1590/s0103-84782008005000003

Cited by 5 publications

(2 citation statements)

References 17 publications

(5 reference statements)

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“…Furthermore, PCV2 and L. intracellularis have been used experimentally to induce PCV-2 associated enteritis in swine [ 10 ]. Both pathogens have been contextually identified in wild boars in Brazil [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Porcine Lawsonia intracellularis Ileitis in Italy and Its Association with Porcine Circovirus Type 2 (PCV2) Infection

D’Annunzio

Ostanello

Muscatello

et al. 2023

Animals

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The objective of this study was to employ a diagnostic algorithm, which involves detecting positive farms by stool PCR followed by PCR and histology/immunohistochemistry on ileum samples, for diagnosing Lawsonia intracellularis proliferative enteritis in Northern Italy. The primary aim was to examine the relationship between the gold standard of L. intracellularis diagnostics, namely histology and immunohistochemistry, and PCR in acute and chronic cases of L. intracellularis enteritides. An additional goal was to investigate the coinfection of L. intracellularis with porcine circovirus type 2 (PCV2). Twenty-eight ileum samples, including four from acute cases and 24 from chronic cases, were collected. PCR yielded positive results in 19 cases (four acute and 15 chronic cases). In comparison, immunohistochemistry was positive in 16 cases (four acute and 12 chronic cases), with an observed agreement of 89%. The findings suggest that performing the two tests in series can increase the specificity of the causal diagnosis. PCR may be used as a screening tool to identify the presence of the microorganism, and only positive cases will be examined by histology and immunohistochemistry to confirm the causative role of L. intracellularis. Co-infection with PCV2 was demonstrate in two out of four acute cases and in two out of 24 chronic cases, providing further evidence to support the hypothesis that when the infection starts with ubiquitous pathogens such as L. intracellularis, it may boost the possibility of PCV2 replication, especially in acute cases. As a result, this may trigger a transition from subclinical to clinical forms of PCV2 disease.

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Section: Introductionmentioning

confidence: 99%

Porcine Lawsonia intracellularis Ileitis in Italy and Its Association with Porcine Circovirus Type 2 (PCV2) Infection

D’Annunzio

Ostanello

Muscatello

et al. 2023

Animals

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“…However, they are not very specific and can also be found in weaned pigs suffering from porcine circovirus type 2 (PCV2) related enteritis (Jensen et al 2006, Zlotowski et al 2008, Szczotka et al 2010, Opriessnig et al 2011. As both pathogens may also cause mild or subclinical infections, and develop very similar clinical symptoms and necropsy lesions, the correct diagnosis is possible only using laboratory methods that provide specific detection in tissue sections (Jensen et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemical detection of Lawsonia intracellularis in tissue sections from pigs

Szczotka¹,

Stadejek²,

Żmudzki³

et al. 2011

Polish Journal of Veterinary Sciences

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The aim of the present study was to develop an immunohistochemical method (IHC) for detection of Lawsonia intracellularis (L. intracellularis) in formalin-fixed, paraffin embedded sections of intestines from pigs and to implement this method in differential diagnosis of swine diseases with diarrhea in postweaning pigs. The study was conducted on 165 sections of intestines (ileum, caecum and colon) collected from 76 pigs, representing 42 Polish pig farms. The animals included in the analysis suffered from diarrhea, with bloody or grey to brown feces, and were suspected of porcine proliferative enteropathy (PPE). Sections of intestines were analyzed for the presence of L. intracellularis by polymerase chain reaction (PCR) and IHC. Among 165 intestinal samples from pigs with diarrhea, L. intracellularis DNA was detected by PCR in 33 (20.0%) samples. In this group, 30 samples (18.2% of all the samples tested) were also found positive in IHC, while only 3 (1.8%) were IHC-negative. One hundred thirty-two (80.0%) samples were negative in both tests. The PCR-and IHC-positive samples originated from 11 pigs, 4-to 20-week old, from 8 farms. L. intracellularis antigen was visualized by IHC mostly in intestinal crypts and/or in mononuclear cells of the lamina propria). The positive signal in epithelial cells was observed close to the luminal borders, creating typical specifically stained rims around the crypt lumina.ąThe results of the present study further confirm the usefulness of IHC in the detection of L. intracellularis antigen in the intestinal tissues.

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Proliferative Enteropathy

Vannucci¹,

Gebhart²,

McOrist³

2019

Diseases of Swine

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Enteritis associated with Lawsonia intracellularis and porcine circovirus type 2 infection in wild boars in Southern Brazil

Abstract: Enterite associada com infecção por

Cited by 5 publications

References 17 publications

Porcine Lawsonia intracellularis Ileitis in Italy and Its Association with Porcine Circovirus Type 2 (PCV2) Infection

Porcine Lawsonia intracellularis Ileitis in Italy and Its Association with Porcine Circovirus Type 2 (PCV2) Infection

Immunohistochemical detection of Lawsonia intracellularis in tissue sections from pigs

Proliferative Enteropathy

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