Cistoplastia experimental em coelhos (Oryctolagus cuniculus) com peritônio bovino conservado em glicerol a 98%

Abstract

IICistoplastia experimental em coelhos (Oryctolagus cuniculus) com peritônio bovino conservado em glicerol a 98% INTRODUÇÃOA utilização de membranas biológicas, no Brasil, teve início a partir da implantação experimental de dura-máter homóloga, conservada em glicerina, na substituição de segmento dural de cães (PIGOSSI, 1967).O peritônio bovino é uma membrana biológica de origem animal, composta quase que exclusivamente de colágeno, possuindo, portanto, baixa antigenicidade. Além disso, já há algum tempo sabe-se que este material apresenta fácil obtenção e estocagem, esterilização viável, praticidade ao I Departamento de Morfologia e Fisiologia Veterinária, Faculdade de Ciências Agrárias e Veterinárias (FCAV), Universidade Estadual Paulista (UNESP), Campus Jaboticabal. Via de Acesso Prof. Paulo Donato Castelanne, s/n, 14884-900, Jaboticabal, SP, Brasil. Email: mrfmachd@fcav.unesp.br. *Autor para correspondência. II Faculdade São Luís, Jaboticabal, SP, Brasil.Recebido para publicação 24.04.07 Aprovado em 11.06.08

Characterization of bladder calculi and urinalysis in rabbits (Oryctolagus cuniculus) treated with mesenchymal adipose-derived stem cells (ADSCs) after partial urinary bladder allotransplantation

“…The animals were monitored daily for water and food intake and no changes in ingestion amount were noted. No signs of peritonitis, suture dehiscence, or rejection of the implant were observed in any subject, results similar to those reported by Oliveira et al (2008). Teixeira et al (2007) reported three deaths between 35 to 50 days after surgery, but the dogs in that study showed signs of implant rejection.…”

“…According to the author, the formation of calcium carbonate calculi in rabbits is usually attributed to an excess of these crystals in the urine, which in turn is caused by an excess of this element in the diet, although the relation between calculi and excess calcium in the diet is still not confirmed. The crystalluria observed in the GCi group was composed of calcium carbonate, consistent with findings of Oliveira et al (2008). In the GCe group, calcium carbonate and triple phosphate were present, which could be explained by urinary alkalinisation in this group compared to the GCi group.…”

“…According to Teixeira et al (2007), who performed allotransplantation in dogs after partial cystectomy, urine changes such as crystalluria and salt deposits, as well as bladder calculi, are common soon after reconstructive surgery of the urinary bladder; however, in their study no such changes were observed 60 days after the transplant surgery. Oliveira et al (2008) reported that implants led to the formation of calculi consisting of calcium carbonate but not of struvite, calcium oxalate, or calcium phosphate, all of which have been observed by other authors. Piechota et al (1998), using rats, observed that the predominant mineral in calculi was struvite.…”

“…In the GCe group, urine density 15 days postoperatively was 1051.67 ± 13.17 and pH 7.92 ± 1.36, while 30 days after surgery urine density was 1054.60 ± 6.31 and pH was 8.40 ± 1.34. Oliveira et al (2008), who also used rabbits, reported a lower mean urinary density (1030) and variation in urine pH between 8.0 and 9.0; mean pH was not reported.…”

“…The most prevalent type of calculus was struvite, which was found in 60 to 100% of the stones from treated rats. Oliveira et al (2008) implanted a patch of bovine peritoneal membrane in rabbits as a substitute for a ventral fragment of the urinary bladder in order to evaluate the process of tissue repair. At each evaluation time point, macroscopic observation revealed the presence of bladder calculi consisting of calcium carbonate.…”