Soroepidemiologia da babesiose em rebanho de bovinos da raça Curraleiro

Juliano, R. S.; Machado, Rosângela Zacarias; Fíoravantí, Maria Clorínda Soares; Andrade, Gabriela; Jayme, Valéria de Sá

doi:10.1590/s0103-84782007000500026

Cited by 7 publications

(6 citation statements)

References 9 publications

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“…Various studies carried out in Brazil have found similar results to ours here. Juliano et al (2007) found prevalence rates higher than 92% on two occasions, in studying the levels of antibodies in Curraleira cattle bred in a cerrado (savanna) area of northeastern Brazil. Guedes Junior et al (2008) found 98.8% prevalence in zebu and crossbred cattle in the northern region, and Trindade et al (2010) observed prevalence of 91.7% in zebu calves in the state of Tocantins in the midwestern region of Brazil.…”

Section: Discussionmentioning

confidence: 95%

Neither quantification by qPCR nor quantitative Elisa can be used to discriminate Angus cattle for resistance/susceptibility to Babesia bovis

Giglioti

Oliveira

Ibelli

et al. 2017

Ticks and Tick-borne Diseases

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With the aim of finding quantitative phenotypic traits that can be used to discriminate the levels of resistance/susceptibility to Babesia bovis, we estimated the repeatability and correlation between the level of infection, determined by the number of copies of a fragment of the gene that encodes cytochrome B (NC mt-cyB) of B. bovis, and the levels of the anti-B. bovis antibodies, in blood samples collected from 51 Angus cattle on two different occasions. Samples with the anticoagulant EDTA were used for DNA extraction and without anticoagulant for separation of the blood serum. The quantification of the NC mt-cyB of B. bovis was carried out by the quantitative PCR technique (qPCR), while the anti-B. bovis IgG antibody titers (S/P) were quantified by the ELISA method. The NC and S/P data were log10-transformed to improve the approximation to the normal distribution and were analyzed using mixed models. The correlations between NC mt-cyB and S/P were estimated, as well as the repeatability values for each trait. The results obtained showed the high sensitivity of the techniques, with 100% of the animals being positive for B. bovis, detected by both the serological and molecular tests. The correlations estimated between NC and S/P were low, 0.10 and 0.12, in the first and second collection, respectively. The repeatability estimated for NC was 0.06, whereas for the S/P it was 0.42. The low correlations between S/P and NC in the two collections demonstrated that the variation in the NC value is independent of the level of antibodies. This results indicated that animals with a higher levels of antibodies against B. bovis in the first collection continued to have a higher levels in the second one. However, the very low values for the repeatability value of NC, and for the correlations between S/P and NC, demonstrates that neither NC or S/P could be used to discriminate animals for resistance/susceptibility to B. bovis.

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Section: Discussionmentioning

confidence: 95%

Neither quantification by qPCR nor quantitative Elisa can be used to discriminate Angus cattle for resistance/susceptibility to Babesia bovis

Giglioti

Oliveira

Ibelli

et al. 2017

Ticks and Tick-borne Diseases

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“…Other regions of the country were considered areas of stability, such as the city of Goiania (SANTOS et al, 2001) in Goiás, the Curraleiros Experimental Station for Bovine Studies (CESBS) also in Goiás (JULIANO et al, 2007), the semiarid region of Bahia (BARROS et al, 2005), and the Forest Zone in Minas Gerais (SALCEDO et al, 1987). Other locations were considered areas of instability, such as the States of Rondonia and Acre in northern Brazil (BRITO et al, 2007) and Londrina in the State of Parana (VIDOTTO et al, 1997).…”

Section: Resultsmentioning

confidence: 99%

“…; the most used are the indirect immunofluorescence assay (IIFA) and the indirect immunoenzimatic ELISA assay (IIEA), as they are sensitive and specific tests and therefore considered appropriate for epidemiological studies of this disease (SANTOS et al, 2001;OSAKI et al, 2002;JULIANO et al, 2007). But a comparative study between IIFA and ELISA demonstrated that the latter was more sensitive and specific, allowing the analysis of a larger number of samples (MARTINS et al, 1996;ARAÚJO et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Detection of antibodies against Babesia bovis and Babesia bigemina in calves from the region of Araguaína, State of Tocantins, Brazil

Trindade

Silva

Teixeira

et al. 2010

Rev. Bras. Parasitol. Vet.

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The aim of this study was to determine the seroprevalence of antibodies against B. bovis and B. bigemina in calves from the region of Araguaína, State of Tocantins, Brazil. In this research we used sera obtained from 506 calves, from both genders and of 8 to 24 months old, to detect antibodies by indirect Enzyme-Linked Immunosorbent Assay (ELISA-test). Statistical analysis of the data was performed using the Chi-square (χ 2 ) test with Yates correction. The seroprevalence obtained was 90.5 and 91.7% for B. bigemina and B. bovis, respectively, characterizing the region as an area of enzootic stability for the species analyzed. The seroprevalence to B. bovis showed higher positivity among calves 19-24 months old.Keywords: Bovine babesiosis, calves, sorology, Tocantins, Brazil. ResumoO objetivo desse estudo foi determinar a soroprevalência de anticorpos anti-B. bovis e anti-B. bigemina em bezerros da região de Araguaína, Estado do Tocantins, Brasil. Nesta pesquisa foram coletadas 506 amostras de soros de bezerros com faixa etária entre 8 e 24 meses, fêmeas e machos, as quais foram processadas pelo ensaio imunoenzimático indireto (ELISA-teste). Como análise estatística utilizou-se o Qui-Quadrado (χ 2 ) com correção de Yates. As prevalências obtidas em bezerros foram de 90,5 e 91,7% para B. bigemina e B. bovis, respectivamente, caracterizando esta região como de estabilidade enzoótica para as espécies analisadas. A soroprevalência para B. bovis apresentou maior positividade entre os bezerros com faixa etária de 19-24 meses.

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“…(SMITH et al, 2000). In Brazil, bovine babesiosis is caused by the apicomplexan intraerythrocytic protozoa parasites Babesia bovis and B. bigemina, which are agents of huge economic importance in tropical and subtropical regions (JULIANO et al, 2007;SIVAKUMAR et al, 2013). Among the agents causing bovine babesiosis, B. bovis is regarded as the most pathogenic species, causing high mortality among susceptible cattle (GAFFAR et al, 2004;BROWN et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Longitudinal evaluation of humoral immune response and merozoite surface antigen diversity in calves naturally infected with Babesia bovis, in São Paulo, Brazil

Matos¹,

Gonçalves

Obregón

et al. 2017

Rev. Bras. Parasitol. Vet.

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Babesiosis is an economically important infectious disease affecting cattle worldwide. In order to longitudinally evaluate the humoral immune response against Babesia bovis and the merozoite surface antigen diversity of B. bovis among naturally infected calves in Taiaçu, Brazil, serum and DNA samples from 15 calves were obtained quarterly, from their birth to 12 months of age. Anti-B. bovis IgG antibodies were detected by means of the indirect fluorescent antibody test (IFAT) and enzyme-linked immunosorbent assay (ELISA). The polymerase chain reaction (PCR) was used to investigate the genetic diversity of B. bovis, based on the genes that encode merozoite surface antigens (MSA-1, MSA-2b and MSA-2c). The serological results demonstrated that up to six months of age, all the calves developed active immunity against B. bovis. Among the 75 DNA samples evaluated, 2, 4 and 5 sequences of the genes msa-1, msa-2b and msa-2c were obtained. The present study demonstrated that the msa-1 and msa-2b genes sequences amplified from blood DNA of calves positive to B. bovis from Taiaçu were genetically distinct, and that msa-2c was conserved. All animals were serologically positive to ELISA and IFAT, which used full repertoire of parasite antigens in despite of the genetic diversity of MSAs.

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Soroepidemiologia da babesiose em rebanho de bovinos da raça Curraleiro

Cited by 7 publications

References 9 publications

Neither quantification by qPCR nor quantitative Elisa can be used to discriminate Angus cattle for resistance/susceptibility to Babesia bovis

Neither quantification by qPCR nor quantitative Elisa can be used to discriminate Angus cattle for resistance/susceptibility to Babesia bovis

Detection of antibodies against Babesia bovis and Babesia bigemina in calves from the region of Araguaína, State of Tocantins, Brazil

Longitudinal evaluation of humoral immune response and merozoite surface antigen diversity in calves naturally infected with Babesia bovis, in São Paulo, Brazil

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