Escherichia coli verotoxigênica: isolamento e prevalência em 60 propriedades de bovinos de leite da região de Pelotas, RS, Brasil

Sandrini, Cecília Nunes Moreira; Pereira, Murilo Anderson; Brod, Claudiomar Soares; Carvalhal, José Beiro; Aleixo, José Antônio Guimarães

doi:10.1590/s0103-84782007000100028

Cited by 10 publications

(10 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Detection of E. coli O157:H7 in Minas Frescal cheese confirms the presence of this pathogen in dairy products marketed in Brazil, and this result is in accordance with previous reports from Cerqueira et al (1999), Irino et al (2005) and Sandrini et al (2007). In spite of these findings, the pathogen was only confirmed in artisan farmhouse cheeses, produced with unpasteurized milk, generally under inappropriate hygienic conditions.…”

Section: Discussionsupporting

confidence: 90%

“…E. coli O157: H7 was detected in 6.8% of feces samples from cattle and 0.2% of dairy cows in Argentina (Padola, et al , 2004; Fernández, et al , 2010), while in the United States, LeJeune et al (2006) isolated this serotype in 0.6% of dairy cows from Ohio State. Undercooked beef, unpasteurized milk and use of water contaminated by cattle manure have been associated to human outbreaks of hemorrhagic colitis and hemolytic uremic syndrome (Hussein and Sakuma, 2005; Sandrini, et al , 2007; Karmali, et al , 2009). …”

Section: Introductionmentioning

confidence: 99%

“…In Brazil, no E. coli O157:H7 food-borne outbreak was reported so far although this serotype has been isolated from sporadic cases of human diarrhea (Irino, et al , 2002) and cattle feces (Riley, et al , 1983; Cerqueira, et al , 1999; Sandrini, et al , 2007; Stella et al , 2008). …”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

PCR and ELISA (VIDAS ECO O157®) Escherichia coli O157:H7 identification in Minas Frescal cheese commercialized in Goiânia, GO

Carvalho¹,

Oliveira-Jr²,

Mesquita³

et al. 2014

Braz. J. Microbiol.

View full text Add to dashboard Cite

Escherichia coli O157:H7 has been incriminated in food poisoning outbreaks and sporadic cases of hemorrhagic colitis and hemolytic uremic syndrome in many countries. Considering the high susceptibility of Minas Frescal cheese to contamination by E. coli O157:H7, the aim of this study was to determine the occurrence of this pathogen through PCR (Polymerase Chain Reaction) and ELISA (VIDAS ECO O157®, bioMérieux, Lyon, France) test. Thirty cheese samples manufactured by artisan farmhouse producers were collected from open-air markets in Goiânia and thirty from industries under Federal Inspection located in Goiás State which trade their products in supermarkets in Goiânia. E. coli O157:H7 was detected in 6.67% samples collected in open air markets using ELISA, and 23,33% with PCR. The pathogen was not detected in samples from industries under Federal Inspection.

show abstract

Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

PCR and ELISA (VIDAS ECO O157®) Escherichia coli O157:H7 identification in Minas Frescal cheese commercialized in Goiânia, GO

Carvalho¹,

Oliveira-Jr²,

Mesquita³

et al. 2014

Braz. J. Microbiol.

View full text Add to dashboard Cite

show abstract

“…In Brazil, Vicente et al (2005) found a prevalence as high as 18.9% (86/454) for serogroup O157 in some herds in São Paulo State. However, Sandrini et al (2007) reported a prevalence of less than 0.3% (3/1127) in dairy cattle from Rio Grande do Sul State and Cerqueira et al (1999) observed a prevalence of 1.5% (3/197) for O157:H7 in healthy dairy cattle from Rio de Janeiro State. The presence of EHEC virulence markers in E. coli isolates represents a potential risk to human health and all O157:H7 isolates possess a common combination of virulence factors: stx2, eae and rfbO157 (Pennington 2010).…”

Section: Discussionmentioning

confidence: 94%

“…Transmission to humans occurs through consumption of undercooked ground (minced) beef, unpasteurized milk, dairy products and vegetables or water contaminated with cattle feces (Cergole-Novella et al 2006, Sandrini et al 2007, Pennington 2010.…”

Section: Introductionmentioning

confidence: 99%

Enterohemorrhagic Escherichia coli O157: H7 from healthy dairy cattle in Mid-West Brazil: occurrence and molecular characterization

Filho

Ferreira

Pinto³

et al. 2014

Pesq. Vet. Bras.

View full text Add to dashboard Cite

Pesq. Vet. Bras. 34(1):24-28, janeiro 2014 24 RESUMO.-[Escherichia coli enterohemorrágica O157: H7 em bovinos leiteiros saudáveis no Centro-Oeste do Brasil: ocorrência e caracterização molecular.] Escherichia coli enterohemorrágica (EHEC) sorotipo O157:H7 representa as principais cepas de E. coli produtoras de toxina Shiga (STEC) relatadas em grandes surtos e doenças graves, tais como colite hemorrágica (CH) e síndrome hemolí-tica urêmica (SHU), potencialmente letais. O objetivo deste estudo foi reportar a ocorrência e caracterização molecular de STEC 0157:H7 isoladas por swab retal de 52 bovinos saudáveis pertencentes a 21 rebanhos leiteiros do Centro--Oeste do Brasil. A detecção dos genes 16SrRNA, stx1, stx2, rfbO157, fliCh7, eae, ehxA, saa, cnf1, chuA, yjaA Enterohemorrhagic Escherichia coli (EHEC) serotype O157:H7 represents the major Shiga toxin-producing E. coli (STEC) strain related to large outbreaks and severe diseases such as hemorrhagic colitis (HC) and the potentially lethal hemolytic uremic syndrome (HUS). The aim of this study was to report the occurrence and molecular characterization of O157:H7 isolates obtained by rectal swab from 52 healthy dairy cattle belonging to 21 farms in Mid-West of Brazil. Detection of 16SrRNA, stx1, stx2, rfbO157, fliCh7, eae, ehxA, saa, cnf1, chuA, yjaA and TSPE4.C2 genes was performed by PCR. The isolates were further characterized by serotyping. Two hundred and sixty E. coli isolates were obtained, of which 126 were characterized as STEC. Two isolates from the same cow were identified as serotype O157:H7. Both isolates presented the stx2, eae, ehxA, saa and cnf1 virulence factor genes and the chuA gene in the phylogenetic classification (virulent group D), suggesting that they were clones. The prevalence of O157:H7 was found to be 1.92% (1/52 animals), demonstrating that healthy dairy cattle from farms in the Mid-West of Brazil are an important reservoir for highly pathogenic E. coli O157:H7.

show abstract

Isolation, prevalence, and risk factors for infection by shiga toxin-producing Escherichia coli (STEC) in dairy cattle

Ferreira

Filho

Pinto

et al. 2014

Trop Anim Health Prod

View full text Add to dashboard Cite

Rectal swabs of 198 Holstein × Gir crossbred beef cattle from 34 milk farms in the central west of Brazil were analyzed from August 2010 to February 2011. Strains of shiga toxin-producing Escherichia coli (STEC) were isolated from 72.73% (144/198) of the animals, on over 97% of the surveyed properties. The molecular characterization indicated the most common toxin gene stx1 in 70.88% of the animals (202/285), followed by 18.95% (54/285) stx1/sxt2, and 10.18% (29/285) stx2. The presence of STEC in animals together with the probable risk factors based on a questionnaire was evaluated in the owners of the evaluated animals. Results showed that the animal category "calves" and production/technification scale "low" of the farm were related to high STEC prevalence in cattle. The season did not significantly affect the presence of STEC in cattle. The STEC strains are considered a major pathogen, causing severe and potentially lethal diseases in humans such as hemorrhagic colitis and hemolytic uremic syndrome. This high prevalence of STEC in dairy cattle poses a significant risk to public health, since these microorganisms can contaminate products intended for human consumption, e.g., water, raw and pasteurized milk, meat products, dairy products, and/or products of plant origin.

show abstract

Escherichia coli verotoxigênica: isolamento e prevalência em 60 propriedades de bovinos de leite da região de Pelotas, RS, Brasil

Abstract: A produção de verotoxinas foi investigada em 1.127 isolamentos de Escherichia coli feitos a partir de 243 bovinos de leite, de água de consumo humano e animal e de amostras de leite de 60 propriedades da bacia leiteira de

Cited by 10 publications

References 19 publications

PCR and ELISA (VIDAS ECO O157®) Escherichia coli O157:H7 identification in Minas Frescal cheese commercialized in Goiânia, GO

PCR and ELISA (VIDAS ECO O157®) Escherichia coli O157:H7 identification in Minas Frescal cheese commercialized in Goiânia, GO

Enterohemorrhagic Escherichia coli O157: H7 from healthy dairy cattle in Mid-West Brazil: occurrence and molecular characterization

Isolation, prevalence, and risk factors for infection by shiga toxin-producing Escherichia coli (STEC) in dairy cattle

Contact Info

Product

Resources

About