Variabilidades fenotípica e genotípica de estirpes de Staphylococcus aureus isoladas em casos de mastite subclínica bovina

Ferreira, Luciano Menezes; Oliveira, Erika de; Zafalon, L. F.; Souza, V. A. B. de

doi:10.1590/s0103-84782006000400028

Cited by 17 publications

(13 citation statements)

References 14 publications

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“…Although a greater number of isolated microorganisms was observed in milk samples obtained during the rainy period (69) than in dry one (50), the season had no significant influence on the frequency of isolation of microorganisms (Table 4). The greater number of isolations during the rainy period may be related to higher temperature and humidity (Table 2), which favors the proliferation of mastitis-causing microorganisms (Ferreira et al, 2006;Zafalon et al, 2008;Andrade et al, 2011). In addition, only 4.00% (2/50) and 13.0% (9/69) of milk samples with microbiological isolation in dry and rainy periods, respectively, showed slight to intense reaction in CMT, in accordance with the statement of Kapronezai et al (2005) that there is high frequency of asymptomatic carriers in the herd or that CMT is not a good screening test for mastitis in buffaloes, due to the occurrence of false-positive and false-negative results.…”

Section: Resultsmentioning

confidence: 99%

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Prevalence and etiology of buffalo mastitis and milk somatic cell count in dry and rainy seasons in a buffalo herd from Analândia, São Paulo State, Brazil

Pizauro¹,

Silva²,

Santana³

et al. 2014

Arq. Bras. Med. Vet. Zootec.

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The aim of this study was to evaluate somatic cell count (SCC), prevalence and etiology of mastitis in a dairy buffalo herd from Analândia, São Paulo State, Brazil, in the dry and rainy seasons. Additionally, antimicrobial susceptibility profile of microorganisms isolated from milk samples was also evaluated. 1,042 milk samples from female Murrah buffaloes in a dairy farm located in Analândia, São Paulo State, Brazil, collected between May 2011 and November 2012 were analyzed. After the mammary gland physical examination, strip cup test and California Mastitis Test (CMT) were performed. Afterwards, 50mL of milk samples from each mammary quarter were collected aseptically for SCC in automatic equipment and microbiological examination. The antimicrobial sensitivity profile to ampicillin, cefoperazone, ceftiofur, enrofloxacin, gentamicin, neomycin, oxacillin, penicillin, and sulfamethoxazole/trimethoprim was evaluated by disk diffusion method. The monthly average temperature and pluviometric index were obtained from "Centro Integrado de Informações Agrometeorológicas" (CIIAGRO) of "Instituto Agronômico de Campinas" (IAC). Milk samples with positive results in the microbiological test showed average SCC of 137,720 cells/mL in the dry period and 190,309 cells/mL in the rainy period. Although a higher number of isolated microorganisms was observed in buffalo milk samples during the rainy period (69/600) compared to the dry period (50/442), the season had no significant effect on the frequency of isolation of microorganisms.

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Section: Resultsmentioning

confidence: 99%

“…Studies also report seasonal influence in isolation of microorganisms which cause mastitis in cows in Brazil (Ferreira et al, 2006;Zafalon et al, 2008) and buffaloes in Turkey (Özenç et al, 2008;Gürler et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Prevalence and etiology of buffalo mastitis and milk somatic cell count in dry and rainy seasons in a buffalo herd from Analândia, São Paulo State, Brazil

Pizauro¹,

Silva²,

Santana³

et al. 2014

Arq. Bras. Med. Vet. Zootec.

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“…destacam-se pela capacidade de ser ou de tornarem-se resistentes a um grande número de antibióticos. Ferreira et al (2006) associaram o agravamento da resistência bacteriana ao uso frequente e indiscriminado de antimicrobianos e aos mecanismos de transferência de resistência entre os micro-organismos. Este fato é comumente observado no campo em surtos de mastite (BRITO et al, 2001).…”

Section: Resultsunclassified

Caracterização fenotípica e molecular de isolados de Staphylococcus spp. obtidos de leite de ovelhas do Município de Chapecó-SC

et al. 2013

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ResumoO presente trabalho teve como objetivos determinar o perfil de sensibilidade aos antimicrobianos e avaliar a presença de mecanismos de resistência antimicrobiana em Staphylococcus spp. (n=36) isolados de mastite em ovelhas do município de Chapecó-SC. O potencial para produção de biofilme foi determinado pelos testes fenotípicos de Agar Vermelho Congo, DAPI e Violeta de Genciana e por teste molecular pela técnica de PCR para a detecção do gene icaD. Para determinar o perfil de resistência aos antimicrobianos, foi realizado o teste de difusão em disco e detecção dos genes de resistência blaZ, mecA, erm (A, B e C) e msrA. O teste da bomba de efuxo foi realizado através do crescimento das culturas em Agar Muller Hinton contendo brometo de etídio. Os resultados mostraram que 1 (2,78%), 36 (100%) e 10 (27,78%) isolados foram considerados produtores de biofilme pelos testes de Agar Vermelho Congo, DAPI e Violeta de Genciana, respectivamente, enquanto que o gene icaD foi observado em apenas 2 (5,55%) isolados. O menor percentual de sensibilidade foi observado para ampicilina (58,33%) e penicilina (58,33%). Os isolados avaliados foram positivos para os genes de resistência blaZ (58,33%) e msrA (11,11%). Nenhum isolado apresentou os genes de resistência erm (A, B, C) e apenas uma amostra foi positiva para o teste da bomba de efluxo. Palavras-chave: Mastite, ovinos, genes de resistência, bomba de efluxo, biofilme, PCR AbstractThis study aimed to determine the antimicrobial susceptibility profile and to assess the presence of mechanisms of antimicrobial resistance in Staphylococcus spp. (n=36) isolated from mastitis in sheep's Chapecó-SC. The potential for biofilm production was determined by phenotypic tests of Congo Red Agar, DAPI and Gentian Violet and by PCR for the detection of icaD gene. To evaluate the antimicrobial resistance testing was performed disk diffusion and detection of resistance genes blaZ, mecA, ermA, ermB and ermC and msrA also performed by PCR.The pump test was conducted by efuxo crop growth Muller Hinton agar containing ethidium bromide. The results showed that 1 (2,78%), 36 (100%) and

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“…Different microorganisms have been isolated from the bovine mastitis. Staphylococcus aureus (Ferreira et al, 2006;Anderson et al, 2006), Streptococcus agalactiae, and Streptococcus dysgalactiae are among the most frequent and prevalent microorganisms, partly due to the contagious mode of transmission. S. aureus is able to produce a variety of extra cellular toxins and virulence factors in the host (Swinkles et al, 2005;Compton et al 2007 a e b).…”

Section: Introductionmentioning

confidence: 99%

Bovine mastitis in the metropolitan area of Curitiba: antibiotic resistance and antimicrobial control of the infection

Parada

Gonçalves

Soccol

et al. 2011

Braz. arch. biol. technol.

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Variabilidades fenotípica e genotípica de estirpes de Staphylococcus aureus isoladas em casos de mastite subclínica bovina

Cited by 17 publications

References 14 publications

Prevalence and etiology of buffalo mastitis and milk somatic cell count in dry and rainy seasons in a buffalo herd from Analândia, São Paulo State, Brazil

Prevalence and etiology of buffalo mastitis and milk somatic cell count in dry and rainy seasons in a buffalo herd from Analândia, São Paulo State, Brazil

Caracterização fenotípica e molecular de isolados de Staphylococcus spp. obtidos de leite de ovelhas do Município de Chapecó-SC

Bovine mastitis in the metropolitan area of Curitiba: antibiotic resistance and antimicrobial control of the infection

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