Effect of acupuncture on TNF-alpha, IL-&lt;FONT FACE=Symbol&gt;1b&lt;/FONT&gt; and IL-10 concentrations in the peritoneal exudates of carrageenan-induced peritonitis in rats

Scognamillo-Szabó, Márcia Valéria Rizzo; Bechara, Gervásio Henrique; Cunha, Fernando de Queiróz

doi:10.1590/s0103-84782005000100016

Cited by 5 publications

(5 citation statements)

References 24 publications

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“…Assessment of the peritoneal fluid also revealed that SP6 acupuncture reduced the peritoneal leakage and MPO activity (a marker of neutrophil content), another important feature of this inflammatory model. These findings corroborate previous reports that acupuncture, performed at other acupoints such as Yintang (HN3), Houhai or Changqiang (GV1), Baihui (GV20) and Zusanli (ST36), respectively, produces anti-inflammatory effects on carrageenan-induced peritonitis and sepsis induced by cecal ligation in rats [1, 28]. In fact, this group reported that acupuncture inhibited neutrophil migration and partially re-established neutrophil migration into the peritoneal cavity after peritonitis induced by carrageenan and sepsis, respectively.…”

Section: Discussionsupporting

confidence: 92%

“…The SP6 acupuncture did not modify IL-1 β and TNF- α levels in peritoneal fluid, but increased IL-10 levels. Scognamillo-Szabó et al [ 28 ] showed that acupuncture performed using a combination of the acupoints Yintang, Houhai and Baihui reduced IL-1 β but did not change TNF- α and IL-10 peritoneal levels in the carrageenan-induced peritonitis model.…”

Section: Discussionmentioning

confidence: 99%

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Involvement of Interleukin-10 in the Anti-Inflammatory Effect of Sanyinjiao (SP6) Acupuncture in a Mouse Model of Peritonitis

Silva

Guginski

Werner

et al. 2011

Evidence-Based Complementary and Alternative Medicine

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In this study, we determined the anti-inflammatory effect of manual acupuncture at the Sanyinjiao or Spleen 6 (SP6) point on carrageenan-induced peritonitis in mice and investigated mechanisms that may underlie this effect. In the first set of experiments, male Swiss mice were allocated into five groups: the control (sterile saline), dexamethasone (DEXA), invasive sham-acupuncture (non-acupoint), SP6 acupuncture and carrageenan-treated groups. Ten minutes after needle retention or 30 min after DEXA treatment, mice received an intraperitoneal injection of carrageenan (750 μg/mouse). After 4 h, total leukocyte and differential cell counts (neutrophils and mononuclear), myeloperoxidase (MPO) activity, vascular permeability and cytokine levels were evaluated. In another set of experiments, adrenalectomized (ADX) mice were used to study the involvement of the adrenal gland on the therapeutic effects of acupuncture. Mice were allocated into two groups: the ADX and sham-operated animals (Sham ADX) that were subdivided into four subgroups each: the control (sterile saline), DEXA, SP6 acupuncture and carrageenan-treated groups. The SP6 and DEXA treatments inhibited the inflammatory cell infiltration, vascular permeability and MPO activity in carrageenan-injected mice. In addition, the SP6 treatment also increased interleukin (IL)-10 levels. In contrast, when the animals were adrenalectomized, the SP6 treatment failed to reduce total leukocyte and the plasma extravasation. In conclusion, this study clearly demonstrates the anti-inflammatory effect of SP6 acupuncture in a model of carrageenan-induced peritonitis. Our results demonstrated that SP6 acupuncture depends of the adrenal glands and increased IL-10 levels to produce its anti-inflammatory action.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Involvement of Interleukin-10 in the Anti-Inflammatory Effect of Sanyinjiao (SP6) Acupuncture in a Mouse Model of Peritonitis

Silva

Guginski

Werner

et al. 2011

Evidence-Based Complementary and Alternative Medicine

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“…It is worth noting that these two cytokines stimulate the systemic release of IL-6 and IL-8, thus amplifying the inflammatory response, since IL-6 is mainly involved in the induction of fever and production of acute phase proteins. 7,29 On the contrary, macrophages produce an anti-inflammatory cytokine that plays an important role in the control of inflammation-IL-10. 30,31 One of our most important findings is that MA at SP6 did not affect IL-1β levels, but decreased TNF-α levels while increasing-or maintaining-IL-10 in the peritoneal fluid (supplementary material).…”

Section: Discussionmentioning

confidence: 99%

“…Acupuncture administered at Yintang, GV1 (Changqiang) and GV20 (Baihui) reduced IL-1β but did not alter peritoneal levels of TNF-α and IL-10 in a carrageenan-induced model of peritonitis and sepsis induced by cecal ligation in rats. 7,29 However, we would emphasize that alternatively activated macrophages (M2) secrete anti-inflammatory cytokines like IL-10, promote healing and recovery, and are stimulated to produce this cytokine by several endogenous factors. 32 In a previous study, we observed that macrophages Acupuncture in Medicine, 39 (4) in the periphery are stimulated by acupuncture and undergo changes in phenotype.…”

Section: Discussionmentioning

confidence: 99%

Acupuncture reduces peripheral and brainstem cytokines in rats subjected to lipopolysaccharide-induced inflammation

et al. 2020

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Background: Lipopolysaccharide (LPS) endotoxins are activators of innate immunity inducing infection and inflammatory responses. Anti-inflammatory drugs can have undesirable side effects. Acupuncture may be an alternative for the treatment of inflammatory processes. Objective: We investigated the potential anti-inflammatory effect of manual acupuncture (MA) at SP6 upon LPS-induced peritonitis in rats. Methods: Peritonitis was induced in rats with an intraperitoneal injection of LPS (0.002, 0.02, 0.2 or 2 µg/kg) in four experimental groups (n = 6 each). A fifth group was injected with sterile saline solution (saline group, n = 6). Four hours after the procedure, peritoneal fluid was collected to determine total cell counts for inflammatory cells, differential leukocyte counts and peritoneal capillary permeability. The LPS dose of 0.02 µg/kg was used in the subsequent experiments as it most successfully induced peritoneal inflammation. Subsequently, five experimental groups (n = 12 rats each) were used: (1) saline, (2) control (untreated LPS group), (3) indomethacin (LPS group treated with indomethacin), (4) NA (LPS group treated with MA at a location not corresponding to any traditional acupuncture point), and (5) SP6 (LPS group treated with verum MA at SP6). Ten minutes after MA or 30 min after indomethacin treatment, the rats received an intraperitoneal injection of LPS. After 4 h, total leukocyte and differential cell counts, myeloperoxidase (MPO) activity, vascular permeability and cytokine levels were evaluated in the peritoneal fluid. Cytokine levels were additionally evaluated in the brainstem. Results: SP6 MA and indomethacin treatments reduced inflammatory cell infiltration, vascular permeability and MPO activity in the LPS-exposed rats. Pre-treatment with indomethacin and SP6 MA decreased tumor necrosis factor (TNF)-α levels and preserved interleukin (IL)-10 in the peritoneal fluid. Indomethacin also reduced IL-6 in the peritoneal fluid. In the brainstem, indomethacin reduced IL-1β, IL-6, TNFα and IL-10, whereas SP6 MA reduced only TNFα and IL-6 levels. Conclusions: This study clearly demonstrates the anti-inflammatory effect of acupuncture, which we believe may involve the activation of anti-inflammatory neural reflexes in the regulation of peritonitis.

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“…But those cytokines came down toward the levels of the healthy group (p<0,05) after treatment with acupuncture, although the level still remained elevating [8]. In some experiments using animal experiments showed that acupuncture therapy using various techniques could decrease the amount of cytokine IL-6 and improve the immune system [11], [12], [13]. Nascimento de Souza et, al also found that acupuncture at ST36 and GV3 is able to promote partial recovery of locomotion, and this improvement could be associated with reduced lesion size and an anti-inflammatory action by modulating the balance of pro and anti -inflammatory cytokines [11].…”

Section: Treatment Anxiety Depression Anxiety Depressionmentioning

confidence: 99%

The Benefit of Renewable Indonesian Traditional Therapyto Enhance Recovery of Physical-Mental Illness

Sudjiwanati¹,

Suparno²

2018

Proceedings of the 5th International Conference on Community Development (AMCA 2018)

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Renewable Indonesian traditional therapies: holomerispot manipulation is a natural, nochemical treatment that can be used to improve mental health and reduce symptoms of physical illness. This study aims to prove the influence of holomerispot manipulation therapy on a key immune mediator: cytokine interleukin-6 (IL-6). The research method used was experimental research with pre and post-therapy repetition with control group. The sample was 20 people divided into 2 groups, which each group got different treatment. Group 1 was the control group and group 2 was the treatment group with holomerispot manipulation and art therapy. The results suggested that the treatment group showed lower IL-6 values compared to the control group. Based on the results of the psychological tests, the treatment group showed a decrease in the level of anxiety and depression, which was better than the control group. The conclusion of this study is that holomerispot manipulation therapy and Art therapy can improve healing and reduce symptoms of mentalphysical illness of the patients. The holomerispot therapy manipulation and art therapy are able to reduce physical-mental illness through the degradation levels of IL-6.

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Effect of acupuncture on TNF-alpha, IL-<FONT FACE=Symbol>1b</FONT> and IL-10 concentrations in the peritoneal exudates of carrageenan-induced peritonitis in rats

Abstract: Efeito da acupuntura sobre a concentração de TNF-α, IL-1β e IL-10 no exsudato peritoneal de ratos com peritonite induzida por carragenina

Cited by 5 publications

References 24 publications

Involvement of Interleukin-10 in the Anti-Inflammatory Effect of Sanyinjiao (SP6) Acupuncture in a Mouse Model of Peritonitis

Involvement of Interleukin-10 in the Anti-Inflammatory Effect of Sanyinjiao (SP6) Acupuncture in a Mouse Model of Peritonitis

Acupuncture reduces peripheral and brainstem cytokines in rats subjected to lipopolysaccharide-induced inflammation

The Benefit of Renewable Indonesian Traditional Therapyto Enhance Recovery of Physical-Mental Illness

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