Avaliação da fidelidade genotípica por marcadores RAPDs de brotações de pereira (Pyrus communis L.) cv. Carrick, regeneradas in vitro

Erig, Alan Cristiano; Schuch, Márcia Wulff

doi:10.1590/s0103-84782003000300009

Cited by 2 publications

(2 citation statements)

References 13 publications

(6 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar results were observed in pear by Erig and Schuch (2003). This result can be explained by the fact that the experimental material originated from explant tissues, in other words, via direct regeneration.…”

Section: Resultssupporting

confidence: 85%

Determining the genetic stability of micropropagated sugarcane using inter-simple sequence repeat markers

Hsie¹,

Brito²,

Nova³

et al. 2015

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. Sugarcane culture is an important source of income for the Brazilian economy. The aim of this study was to identify somaclonal variation in sugarcane varieties RB943365 and RB92579 arising from micropropagation using inter-simple sequence repeat (ISSR) DNA markers. The evaluated plants were generated from the in vitro propagation of shoot tips grown in MS medium supplemented with vitamins, myoinositol, glycine, and sucrose, without the use of growth regulators. Fifteen consecutive subcultures with intervals of 14 days were carried out, and DNA was extracted from young leaves obtained from each of the subcultures. The DNA was amplified with ISSR markers and separated by electrophoresis on 2% agarose gels. No evidence of polymorphism was observed in subcultures of the varieties analyzed, suggesting the absence of somaclonal variants. In this way, the ISSR marker was efficient at analyzing somaclonal variation, and in vitro propagation of sugarcane can be considered efficient for 15 consecutive subcultures of the varieties analyzed.

show abstract

Section: Resultssupporting

confidence: 85%

Determining the genetic stability of micropropagated sugarcane using inter-simple sequence repeat markers

Hsie¹,

Brito²,

Nova³

et al. 2015

Genet. Mol. Res.

View full text Add to dashboard Cite

show abstract

“…The polymorphism was not occurred in regenerated shoots from axillary buds, apical meristem, and leaf whorl disk during the analysis. Erig and Schuch (2003) and Hsie et al (2015) observed similar results in sugarcane and showed 100% true-to-type plantlets. The present results enlighten the fact that plants developed from direct organogenesis are highly stable than those obtained from indirect oraganogenesis (callus) (Kamenickà and Rypák, 1989).…”

Section: Resultssupporting

confidence: 61%

Plant regeneration from direct and indirect organogenesis and assessment of genetic fidelity in Saccharum officinarum using DNA-based markers

Thorat¹,

Sonone²,

Choudhari³

et al. 2018

Biosci. Biotech. Res. Comm

View full text Add to dashboard Cite

Sugarcane has acquired signifi cant importance in the world economy because of the sugar and ethanol production. Therefore, rapid multiplication of outstanding sugarcane variety is necessary in developing countries. The aim of this investigation was to compare regeneration effi ciency of various explants and genetic fi delity of regenerated plantlets certifi ed by using DNA-based molecular markers, that is, random amplifi ed length polymorphism (RAPD) and inter simple sequence repeats (ISSR). The sugarcane plantlets were obtained through direct (axillary buds, apical meristem, and leaf whorl disk) and indirect (callus culture) shoot organogenesis from the variety Co 86032. Among all the explants, highest shoot forming ability was observed in axillary buds showed 97.66±0.66% shoot formation and the highest number of shoots per explants (4.33±0.24) and a total number of regenerated shoots (173.00±8.11) were observed in the leaf whorl disk. Morphological variation was not observed among the regenerated plants from various explants and therefore, genomic DNA was isolated from fresh leaves and genetic fi delity assessment was carried out using RAPD and ISSR. Both the markers produced 1368 and 2271 bands, respectively, including all the tested plants, indicates that plants derived from direct organogenesis did not show any polymorphism. However, a genetic variation has been observed in the plants derived from callus and showed 4.54% polymorphism during analysis. The results suggested that plants regenerated from direct organogenesis are of more true-to-type, whereas genetic variation occurs during indirect organogenesis. Combination of RAPD along with ISSR can be used for detection of genetic variation in the early stage in sugarcane micropropagation. High performance of regeneration and low risk of genetic variation ascertains the effi ciency of this operation.

show abstract

Avaliação da fidelidade genotípica por marcadores RAPDs de brotações de pereira (Pyrus communis L.) cv. Carrick, regeneradas in vitro

Cited by 2 publications

References 13 publications

Determining the genetic stability of micropropagated sugarcane using inter-simple sequence repeat markers

Determining the genetic stability of micropropagated sugarcane using inter-simple sequence repeat markers

Plant regeneration from direct and indirect organogenesis and assessment of genetic fidelity in Saccharum officinarum using DNA-based markers

Contact Info

Product

Resources

About