Atividade neutralizante anti-herpesvírus bovino tipos 1 (BHV-1) e 5 (BHV-5) no soro de bovinos imunizados com vacinas contra o BHV-1

Vogel, Fernanda Silveira Flores; Flores, Eduardo Furtado; Weiblen, Rudi; Kunrath, Cíntia Farias

doi:10.1590/s0103-84782002000500022

Cited by 20 publications

(25 citation statements)

References 7 publications

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“…However, as may be expected, the levels of cross-reactivity were higher when serum samples were tested against BoHV-5 isolates than against BoHV-1 isolates. The serological cross-reactivity between BoHV-1 and BoHV-5 has also been demonstrated in other vaccine studies (Petzhold et al 2001, Vogel et al 2002, Silva et al 2007a.…”

Section: Discussionsupporting

confidence: 58%

“…Based on biological properties, alphaherpesviruses are suitable for the development of DIVA vaccines, which can be used either as killed or live vaccines . Due to cross reactivity between BoHV-1 and -5 and the lack of specific BoHV-5 vaccines, vaccination against BoHV-1 may be indicated to protect against BoHV-5 infection and disease (Cascio et al 1999, Vogel et al 2002, Del Medico Zajac et al 2006. Cattle immunized with BoHV-1 and challenged with BoHV-5 showed a reduced virus shedding and were partially protected from clinical disease (Cascio et al 1999, Del Medico Zajac et al 2006, Silva et al 2006.…”

Section: Discussionmentioning

confidence: 99%

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Immunogenicity of an inactivated bovine herpesvirus type 5 strain defective in thymidine kinase and glycoprotein E

et al. 2010

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RESUMO.-[Imunogenicidade de uma cepa inativada do herpesvírus bovino tipo 5 defectiva na timidina quinase e glicoproteína E.] A imunogenicidade de vacina experimental inativada, produzida com uma cepa do herpesvírus bovino tipo 5 defectiva nos genes da timidina quinase e glicoproteína E (BoHV-5 gE/TKΔ) foi avaliada em bovinos e o resultado foi comparado com a resposta induzida pela cepa parental do The immunogenicity of an inactivated, experimental vaccine based on a bovine herpesvirus type 5 strain defective in thymidine kinase and glycoprotein E (BoHV-5 gE/ TKΔ) was evaluated in cattle and the results were compared with a vaccine containing the parental BoHV-5 strain (SV507/99). To formulate the vaccines, each virus (wildtype SV507/99 and BoHV-5 gE/TKΔ) was multiplied in cell culture and inactivated with binary ethyleneimine (BEI). Each vaccine dose contained approximately of 10 7.5 TCID 50 of inactivated virus mixed with an oil-based adjuvant (46:54). Forty calves, 6 to 9-monthsold, were allocated into two groups of 20 animals each and vaccinated twice (days 0 and 22pv) by the subcutaneous route with either vaccine. Serum samples collected at day 0 and at different intervals after vaccination were tested for virus neutralizing (VN) antibodies against the parental virus and against heterologous BoHV-5 and BoHV-1 isolates. The VN assays demonstrated seroconversion to the respective homologous viruses in all vaccinated animals after the second vaccine dose (mean titers of 17.5 for the wildtype vaccine; 24.1 for the recombinant virus). All animals remained reagents up to day 116 pv, yet showing a gradual reduction in VN titers. Animals from both vaccine groups reacted in similar VN titers to different BoHV-1 and BoHV-5 isolates, yet the magnitude of serological response of both groups was higher against BoHV-5 field isolates. Calves vaccinated with the recombinant virus did not develop antibodies to gE as verified by negative results in a gE-specific ELISA, what would allow serological differentiation from naturally infected animals. Taken together, these results indicate that inactivated antigens of BoHV-5 gE/TK recombinant virus induced an adequate serological response against BoHV-5 and BoHV-1 and thus can be used as an alternative, differential vaccine candidate. inativados emulsionado em adjuvante oleoso (46:54). Quarenta bezerros de raças cruzadas, com idade entre seis a nove meses, foram alocados em dois grupos de 20 animais cada e vacinados duas vezes (dia 0 e 22 pv) pela via subcutânea com uma das vacinas. Amostras de soro foram coletadas no dia 0 e a vários intervalos após vacinação para a pesquisa de anticorpos neutralizantes frente ao vírus homólogo ou frente a isolados de BoHV-5 e BoHV-1. Os testes de soroneutralização (SN) demonstraram que todos os animais soroconverteram após a segunda dose da vacina (títulos médios de 17,5 para o grupo SV507/99; 24,1 para o grupo BoHV-5 gE/TKΔ). Todos os animais mantiveram níveis de anticorpos neutralizantes até o dia 116 pv, no entanto foi observada uma redução ...

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Section: Discussionsupporting

confidence: 58%

Section: Discussionmentioning

confidence: 99%

Immunogenicity of an inactivated bovine herpesvirus type 5 strain defective in thymidine kinase and glycoprotein E

et al. 2010

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“…The antigenic similarity between BoHV-1 and BoHV-5 is such that most serological tests are unable to distinguish between the antibody responses to each of these viruses. As a consequence, the real seroprevalence of BoHV-1 and BoHV-5 infections is rather difficult to predict in regions where the two viruses co-circulate (d'Offay et al 1994, Vogel et al 2002. Nevertheless, in addition to the vast serological data, many clinic-pathological and virological reports have demonstrated the occurrence of both BoHV-1 and BoHV-5 infection and associated clinical diseases in our country (Riet-Correa et al 1989, Weiblen et al 1989, Flores et al 1998, Salvador et al 1998, Gomes et al 2002.…”

Section: Discussionmentioning

confidence: 98%

“…BoHV-5 has been identified in outbreaks of neurological disease in cattle in several Brazilian states, including Rio Grande do Sul (Riet-Correa et al 1989, Weiblen et al 1989, Roehe et al1998, Mato Grosso do Sul (Salvador et al 1998) and Minas Gerais (Gomes et al 2002). The seroprevalence of BoHV-5 infection among Brazilian cattle is difficult to ascertain with the routine serological tests due to the high percentage of crossreactivity with BoHV-1 what prevents the distinction between the antibody response to BoHV-1 and BoHV-5 (Bratanich et al 1991, Vogel et al 2002.…”

Section: Introductionmentioning

confidence: 99%

A Retrospective Search for Bovine Herpesvirus 5 (Bohv-5) in the Brain of Cattle Affected by Neurological Disease in Rio De Janeiro State

Pinto

Leite²,

Romijn³

et al. 2014

VR&R

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Bovine herpesvirus 5 (BoHV-5) is an important agent of meningoencephalitis in cattle and has been identified in outbreaks of neurological disease in several Brazilian states. However, no report of BoHV-5-associated neurological disease in Rio Janeiro state (RJ) has been published to date. This article reports a retrospective investigation for BoHV-5 performed in 146 brain specimens of cattle affected by neurological disease, submitted to the virology laboratory of the Empresa de Pesquisa Agropecuária do Estado do Rio de Janeiro (PESAGRO-RJ) between 1998 and 2001. Most specimens came from herds in the RJ state (130 or 89%) and a few from Minas Gerais counties (16 or 11%), close to the RJ border. All brain samples examined in this study were previously diagnosed negative for rabies virus. The specimens were submitted to PCR/nested-PCR for BoHV-5 sequences and virus isolation. From 146 analysed brains samples 48 (33%) were positive in at least one of the tests (PCR or virus isolation), 20 samples (14%) were positive in both assays, 19 samples (13%) were positive in Nested-PCR (n-PCR) and nine samples (6%) were characterized as BoHV-5 after virus isolation. The northeast region of the RJ contributed with most clinical specimens (92/146 or 63%) and presented the highest positivity (28/92 or 30%). These results demonstrated BoHV-5 association with neurological disease in bovine herds of RJ and MG and should therefore be included in differential diagnosis of neurological disease in cattle. The limitations of each of the diagnostic assays used here in led us to recommend the concomitant use of PCR and virus isolation to improve the sensitivity of the diagnosis of BoHV-5 infection.

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“…In spite of the differences in the envelope glycoproteins, the traditional serological techniques and the majority of BHV-1 and BHV-5 monoclonal antibodies (mAbs) are unable to distinguish between these two viruses (16,17). Likewise, extensive serological cross-reactivity has been demonstrated between BHV-1 and BHV-5 (17)(18)(19). Nevertheless, the occurrence of cross-protection between BHV-1 and BHV-5 is still controversial (8,17,(19)(20)(21)(22).…”

Section: Introductionmentioning

confidence: 99%

Production and characterization of monoclonal antibodies to a Brazilian bovine herpesvirus type 5

Oldoni

Weiblen

et al. 2004

Braz J Med Biol Res

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Antigens of a bovine herpesvirus type 5 (BHV-5), isolated from a cow with a neurological infection in Rio Grande do Sul State, Brazil, were used to immunize BALB/c mice to produce monoclonal antibodies (mAbs). Eleven hybridomas secreting mAbs directed at BHV-5 antigens were obtained after two fusions and screening of 356 hypoxanthine-aminopterin-thymidine-resistant clones. The mAbs reacted at dilutions up to 1:500 (hybridoma culture supernatant) and up to >1:10,000 (ascitic fluid) in an indirect fluorescent antibody assay (IFA) and in immunoperoxidase staining of BHV-5-infected cells. Four mAbs (1D12, 2E2, 2G10 and 4E4) showed virus-neutralizing activity against the parental BHV-5 isolate. Five mAbs (1F3, 2A6, 2F9, 2G10 and HB24L) reacted in Western immunoblotting with a protein of approximately 90 kDa. Three other mAbs (2E2, 3D6 and 4E4) reacted in IFA with antigens of a BHV-1 mutant glycoprotein Cnegative strain, demonstrating that they are directed at a viral antigen other than glycoprotein C. The eleven mAbs tested reacted with 20 BHV-5 field isolates and nine mAbs reacted with 10 BHV-1 isolates. Two mAbs (1F3 and 2F9) failed to react with BHV-1 field isolates, although they displayed a weak and nonreproducible reaction with the BHV-1 reference strain Los Angeles. These mAbs may be very useful in distinguishing between BHV-1 and BHV-5 infections since most of the traditional reagents and techniques are unable to do so. One mAb (2F9) was shown to bind to viral antigens by immunohistochemistry of histological sections of the brain of a BHV-5-infected calf. These results demonstrate that the mAbs produced here are suitable for use in a variety of immunological techniques and therefore may be useful for diagnostic and research purposes.

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Atividade neutralizante anti-herpesvírus bovino tipos 1 (BHV-1) e 5 (BHV-5) no soro de bovinos imunizados com vacinas contra o BHV-1

Cited by 20 publications

References 7 publications

Immunogenicity of an inactivated bovine herpesvirus type 5 strain defective in thymidine kinase and glycoprotein E

Immunogenicity of an inactivated bovine herpesvirus type 5 strain defective in thymidine kinase and glycoprotein E

A Retrospective Search for Bovine Herpesvirus 5 (Bohv-5) in the Brain of Cattle Affected by Neurological Disease in Rio De Janeiro State

Production and characterization of monoclonal antibodies to a Brazilian bovine herpesvirus type 5

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