Amino acids and carbohydrates absorption by Na+-dependent transporters in the pyloric ceca of Hoplias malabaricus (Erythrinidae)

Vieira, Vinícius da Fonseca; Baldisserotto, Bernardo

doi:10.1590/s0103-84782001000500009

Cited by 2 publications

(1 citation statement)

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“…Hence, in fish fed a low‐cohesion diet, the rate of passage of the food from the stomach to the intestine may be initially accelerated, as chyme will rapidly form in the stomach and pass into the proximal intestine. Upon arrival, further enzymatic degradation occurs and the processes of nutrient (Vieira & Baldisserotto 2001) and water absorption (Rawdon & Cornish 1973; Veillette 2004) begin. Once this chyme reaches the proximal intestine, an intestinal brake is activated via nutritive chemoreception (Jobling 1987; Olsson & Holmgren 2001).…”

Section: Discussionmentioning

confidence: 99%

Development and physiology of gastric dilation air sacculitis in Chinook salmon, Oncorhynchus tshawytscha (Walbaum)

Forgan

Forster

2007

Journal of Fish Diseases

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The syndrome known as gastric dilation air sacculitis (GDAS) has previously been shown to affect Chinook salmon, Oncorhynchus tshawytscha, in seawater (SW) aquaculture. Feed and osmoregulatory stress have been implicated as potential epidemiological co-factors. The development and physiology of GDAS was investigated in SW and freshwater (FW) adapted smolts. Diet A (low-cohesion pellets) and diet B (high-cohesion pellets) were fed to both FW- and SW-adapted fish. GDAS was induced only in the SW trial on feeding diet A. Stimulated gastro-intestinal (GI) smooth muscle contractility, and fluid transport by the pyloric caeca were different in GDAS-affected fish, which also showed osmoregulatory dysfunction. Cardiac stomach (CS) smooth muscle contractility in response to acetylcholine and potassium chloride (KCl) was significantly reduced in fish fed diet A relative to controls from weeks 3-5. In contrast, maximal pyloric sphincter (PS) circular smooth muscle contraction in response to KCl was significantly elevated in fish fed diet A in weeks 4 and 5. Serum osmolality was elevated in GDAS-affected fish from week 2 of the SW trial. Fluid transport from the mucosal to serosal surface of isolated pyloric caeca was significantly reduced in weeks 3, 4 and 5 in SW fish fed diet A. Gastric evacuation from the stomach of healthy fish was shown to be significantly different when diets of low- and high-cohesion were fed. The results are consistent with the intestinal brake playing a role in the development of the disease.

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Section: Discussionmentioning

confidence: 99%

Development and physiology of gastric dilation air sacculitis in Chinook salmon, Oncorhynchus tshawytscha (Walbaum)

Forgan

Forster

2007

Journal of Fish Diseases

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SLC transporters ASCT2, B⁰AT1‐like, y⁺LAT1, and LAT4‐like associate with methionine electrogenic and radio‐isotope flux kinetics in rainbow trout intestine

Masagounder

Loewen

2019

Physiol Rep

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Methionine (Met) is an important building block and metabolite for protein biosynthesis. However, the mechanism behind its absorption in the fish gut has not been elucidated. Here, we describe the fundamental properties of Met transport along trout gut at µmol/L and mmol/L concentration. Both electrogenic and unidirectional DL‐[14C]Met flux were employed to characterize Met transporters in Ussing chambers. Exploiting the differences in gene expression between diploid (2N) and triploid (3N) and intestinal segment as tools, allowed the association between gene and methionine transport. Specifically, three intestinal segments including pyloric caeca (PC), midgut (MG), and hindgut (HG) were assessed. Results at 0–150 µmol/L concentration demonstrated that the DL‐Met was most likely transported by apical transporter ASCT2 (SLC1A5) and recycled by basolateral transporter y+LAT1 (SLC7A7) due to five lines of observation: (1) lack of Na+‐independent kinetics, (2) low expression of B0AT2‐like gene, (3) Na+‐dependent, high‐affinity (K m, µmol/L ranges) kinetics in DL‐[14C]Met flux, (4) association mRNA expression with the high‐affinity kinetics and (5) electrogenic currents induced by Met. Results at 0.2–20 mmol/L concentration suggested that the DL‐Met transport is likely transported by B0AT1‐like (SLC6A19‐like) based on gene expression, Na+‐dependence and low‐affinity kinetics (K m, mmol/L ranges). Similarly, genomic and gene expression analysis suggest that the basolateral exit of methionine was primarily through LAT4‐like transporter (SLC43A2‐like). Conclusively, DL‐Met uptake in trout gut was most likely governed by Na+‐dependent apical transporters ASCT2 and B0AT1‐like and released through basolateral LAT4‐like, with some recycling through y+LAT1. A comparatively simpler model than that previously described in mammals.

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Amino acids and carbohydrates absorption by Na+-dependent transporters in the pyloric ceca of Hoplias malabaricus (Erythrinidae)

Abstract: SUMMARY Information about amino acids and carbohydrate absorption in fish is important to formulate an adequate diet to obtain optimal growth. Therefore, the objective of this study was to investigate if Na+-dependent transporters are involved on the absorption of glycine

Cited by 2 publications

References 13 publications

Development and physiology of gastric dilation air sacculitis in Chinook salmon, Oncorhynchus tshawytscha (Walbaum)

Development and physiology of gastric dilation air sacculitis in Chinook salmon, Oncorhynchus tshawytscha (Walbaum)

SLC transporters ASCT2, B⁰AT1‐like, y⁺LAT1, and LAT4‐like associate with methionine electrogenic and radio‐isotope flux kinetics in rainbow trout intestine

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Amino acids and carbohydrates absorption by Na+-dependent transporters in the pyloric ceca of Hoplias malabaricus (Erythrinidae)

Abstract: SUMMARY Information about amino acids and carbohydrate absorption in fish is important to formulate an adequate diet to obtain optimal growth. Therefore, the objective of this study was to investigate if Na+-dependent transporters are involved on the absorption of glycine

Cited by 2 publications

References 13 publications

Development and physiology of gastric dilation air sacculitis in Chinook salmon, Oncorhynchus tshawytscha (Walbaum)

Development and physiology of gastric dilation air sacculitis in Chinook salmon, Oncorhynchus tshawytscha (Walbaum)

SLC transporters ASCT2, B0AT1‐like, y+LAT1, and LAT4‐like associate with methionine electrogenic and radio‐isotope flux kinetics in rainbow trout intestine

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SLC transporters ASCT2, B⁰AT1‐like, y⁺LAT1, and LAT4‐like associate with methionine electrogenic and radio‐isotope flux kinetics in rainbow trout intestine