Clínica e hematologia de Bos indicus, Bos taurus e Bubalus bubalis inoculados com oocistos de Toxoplasma gondii (Apicomplexa: Toxoplasmatinae)

Oliveira, Francisco Carlos Rodrigues de; Costa, Alvimar José da; Sabatini, Gustavo Adolfo

doi:10.1590/s0103-84782001000400010

Cited by 9 publications

(7 citation statements)

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“…Cows showed 29.1% (35/120) of prevalence for T. gondii. Seroepidemiological surveys for toxoplasmosis in cattle showed anti-T. gondii antibody occurrence ranged from 1.03 to 71% in Brazil (MARANA et al,1995;GONDIM et al, 1999;GARCIA et al, 1999;SANTOS et al, 2008;MOURA et al, 2010;FRAZÃO-TEIXEIRA & OLIVEIRA, 2011), and 0 to 91% in some parts of the world (DUBEY & STREITEL, 1976;HASHEMI-FESHARKI, 1996;MORE et al, 2008;OPSTEEGH et al, 2011). Therefore, caution should be taken when the results of prevalence studies are being evaluated, since the differences in results might be directly related to the serological techniques employed, the cut-off values, sample size, and the type (breed and/or species) of animal that is being investigated.…”

Section: Resultsmentioning

confidence: 99%

“…OLIVEIRA et al (2001) The main titer observed in the present study was 50 (n=23), following by 100 (n=10), 200 (n=1) and 400 (n=1). The correlation between antibodies anti-T. gondii and the presence of tissue cysts is know in sheep and pigs (OPSTEEGH et al, 2010), however, this is not know in cattle (OPSTEEGH et al, 2011). These authors described that the risk of human infection is higher from seronegative than seropositive cattle, by the fact that they detected DNA from T. gondii from negative animals and did not detect from positive animals.…”

Section: Resultsmentioning

confidence: 99%

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Serum occurrence of anti-Toxoplasma gondii antibodies in dairy cows slaughtered in an abattoir for human consume

Macedo

Barros

et al. 2012

Cienc. Rural

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Serum occurrence of anti-Toxoplasma gondii antibodies in dairy cows slaughtered in an abattoir for human consume

Macedo

Barros

et al. 2012

Cienc. Rural

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“…After inoculation of 2.0 x 10 5 sporulated T. gondii oocysts of strain P, Oliveira et al (2001) found that the mean hematological values generally remained within the normal limits for Bos taurus, Bos indicus and Bubalus bubalis. However, B. taurus appeared to be more sensitive to the parasite than B. indicus and B. bubalis, and showed the highest mean values for parasitemia and more evident temperature peaks.…”

Section: Studies On T Gondii Infection In Cattle and Its Importance mentioning

confidence: 84%

“…On the other hand, B. indicus was more resistant and showed lower mean values for leukocytes and lymphocytes than B. taurus and B. bubalis. Despite this, according to these authors, there was transient leukocytosis in all species between 14-21 days after inoculation (DAI) and 35-63 DAI, which may have represented proliferation of the parasite in new sites of the organism (Oliveira et al, 2001). Esteban-Redondo et al (1999) investigated calves that had been orally infected with 10 3 and 10 5 sporulated T. gondii oocysts, of isolate M3.…”

Section: Studies On T Gondii Infection In Cattle and Its Importance mentioning

confidence: 99%

Toxoplasma gondii in cattle in Brazil: a review

Gomes

Krawczak

Oliveira

et al. 2020

Rev. Bras. Parasitol. Vet.

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Toxoplasma gondii is an apicomplexan protozoan that is frequently found in both humans and animals worldwide. The aim of this review was to list important aspects of Toxoplasma gondii infection in cattle in Brazil. The frequency of occurrence of T. gondii antibodies in Brazilian cattle ranges from 1 to 89.1%, depending on the region evaluated, based on data from 1978 to 2018. However, some characteristics of T. gondii infection in cattle remain uncertain, such as the role of meat intake in transmitting the parasite to humans. Most information regarding T. gondii infection among Brazilian cattle is limited to evaluations of the frequency of occurrence of antibodies. About 70% of the diagnoses of infection in these ruminants in Brazil are made via the indirect fluorescence antibody test (IFAT). Nevertheless, little is known about the population structure of this protozoan in cattle. It is necessary to expand the studies on toxoplasmosis in cattle, in order to better understand T. gondii infection in these animals and its implications for Brazilian public health.

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“…al., 2013), enquanto que os resíduos hidrofóbicos, especialmente os aromáticos, podem interagir e possivelmente penetrar na bicamada fosfolipídica, resultando na sua desestabilização (NÚÑEZ et al, 2001). Esta ação está diretamente ligada com a forma dimérica das PLA2-Lys49 em solução (FRANCIS et al, 1991;DA SILVA GIOTTO et al, 1998;OLIVEIRA et al, 2001).…”

Section: -Leishmanioseunclassified

Anticorpos IgY policlonais anti-fosfolipase A2: Ferramenta auxiliar para o estudo da ação anti-parasitária

Borges¹

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Activities of phospholipases (PLAs) have been linked to pathogenesis in various microorganisms. These are involved in processes of cell invasion, virulence, survival and remodeling / modification of the host cell phospholipid composition so the interest in these enzymes as potential targets that could contribute to the control of parasite survival and proliferation. Chicken eggs immunized with BnSP-7, a Lys49 phospholipase A2 (PLA2) homologue from Bothrops pauloensis snake venom, represent an excellent source of polyclonal antibodies with potential inhibitory activity on parasite PLAs. Herein, we report the production, characterization and anti-parasitic effect of IgY antibodies from egg yolks of hens immunized with BnSP-7. Produced antibodies presented increasing avidity and affinity for antigenic toxin epitopes throughout immunization, attaining a plateau after 4 weeks. Pooled egg yolks-purified anti-BnSP-7 IgY antibodies were able to specifically recognize different PLA2s from Bothrops pauloensis and Bothrops jararacussu venom. Antibodies also neutralized BnSP-7 cytotoxic activity in C2C12 cells. In addition, the antibodies recognized targets in Leishmania (Leishmania) amazonensis and Toxoplasma gondii extracts by ELISA and immunofluorescence assays. Anti-BnSP-7 IgY antibodies were cytotoxic to T. gondii tachyzoites and L. (L.) amazonensis promastigotes were able to decrease the proliferation of both parasites treated prior to infection and inhibit the invasion of promentigotes forms of L. (L.) amazonensis. Finally, anti-BnSP-7 antibodies were used to construct a nanocomposite modified graphite immunosensor, that could detect BnSP-7 and targets in L. (L.) amazonensis extract. These data suggest that anti-BnSP-7 IgY is an important tool for discovering new parasite targets, blocking parasitic effects and serving as a diagnostic tool.

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Clínica e hematologia de Bos indicus, Bos taurus e Bubalus bubalis inoculados com oocistos de Toxoplasma gondii (Apicomplexa: Toxoplasmatinae)

Abstract: RESUMO Três animais de cada espécie (Bos indicus, Bos taurus e Bubalus bubalis) foram inoculados, via oral, com

Cited by 9 publications

References 9 publications

Serum occurrence of anti-Toxoplasma gondii antibodies in dairy cows slaughtered in an abattoir for human consume

Serum occurrence of anti-Toxoplasma gondii antibodies in dairy cows slaughtered in an abattoir for human consume

Toxoplasma gondii in cattle in Brazil: a review

Anticorpos IgY policlonais anti-fosfolipase A2: Ferramenta auxiliar para o estudo da ação anti-parasitária

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