Leishmaniose cutânea em cobaias (Cavia porcellus)

Ecco, Roselene; Langohr, Ingeborg M.; Schossler, João Eduardo Wallau; Barros, Severo Sales de; Barros, Claudio S.L.

doi:10.1590/s0103-84782000000300027

Cited by 5 publications

(6 citation statements)

References 5 publications

(6 reference statements)

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“…Under natural conditions, this infection presented a characteristic chronic proinflammatory infiltrate common to most Leishmania species ( Marques et al, 2017 ). In the previous studies with L. enriettii from Brazil, this pattern was also observed during experimental conditions ( Paraense, 1953 ; Bryceson et al, 1974 ; Ecco et al, 2000 ). Microscopically, those previous reports showed that a proinflammatory infiltrate with parasitized macrophages was very common.…”

Section: Discussionsupporting

confidence: 66%

Salivary Gland Extract Modulates the Infection of Two Leishmania enriettii Strains by Interfering With Macrophage Differentiation in the Model of Cavia porcellus

et al. 2018

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The subgenus Mundinia includes several Leishmania species that have human and veterinary importance. One of those members, Leishmania Mundinia enriettii was isolated from the guinea pig Cavia porcellus in the 1940s. Several histopathological studies have already been performed in this species in the absence of salivary gland extract (SGE), which are determinant and the early and future events of the infection. Our main hypothesis is that SGE could differentially modulate the course of the lesion and macrophage differentiation caused by avirulent and virulent L. enriettii strains. Here, the C. porcellus nasal region was infected using needles with two strains of L. enriettii (L88 and Cobaia) in the presence/absence of SGE and followed for 12 weeks. Those strains vary in terms of virulence, and their histopathological development was characterized. Some L88-infected animals could develop ulcerated/nodular lesions, whereas Cobaia strain developed non-ulcerated nodular lesions. Animals experimentally inoculated developed a protuberance and/or lesion after the 4th and 5th weeks of infection. Macroscopically, the size of lesion in L88-infected animals was smaller in the presence of SGE. Remarkable differences were detected microscopically in the presence of SGE for both strains. After the 6th and 7th weeks, L88-infected animals were heavily parasitized with an intense inflammatory profile bearing amastigotes and pro-inflammatory cells compared to those infected by Cobaia strain. Morphometry analysis revealed that L1+ macrophages were abundant in the L88 infection, but not in the Cobaia infection. In the presence of SGE, an increased CD163+ macrophage infiltrate by both strains was detected. Interestingly, this effect was more pronounced in Cobaia-infected animals. This study showed the role of SGE during the course of L. enriettii (strains L88 and Cobaia) infection and its role in modulating macrophage attraction to the lesion site. SGE decreased L1+ macrophages and this may favor an escaping mechanism for L88 parasites. On the other hand, in the presence of SGE, an increase in CD163+ cells during Cobaia infection may be important for its control. Although both strains healed at the end of the infection, the role of SGE was determinant for the kinetics of the immunopathological events in this dermotropic species.

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Section: Discussionsupporting

confidence: 66%

Salivary Gland Extract Modulates the Infection of Two Leishmania enriettii Strains by Interfering With Macrophage Differentiation in the Model of Cavia porcellus

et al. 2018

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“…The epidemiological data, the clinical picture, the macroscopic lesions, and the histopathology observed in the guinea pigs of the present outbreak bear a close similarity to those described by several authors for the mucocutaneous leishmaniasis by Leishmania enriettii (Medina 1946, Muniz & Medina 1948, Paraense 1952, 1953, Bray et al 1969, Bryceson et al 1974, Kanan 1975a, 1975b, Machado et al 1994, Lainson 1997, Ecco et al 2000, Fighera et al 2003, Paranaiba et al 2017. Also, the agent was confirmed as L. enriettii by PCR.…”

Section: Discussionsupporting

confidence: 87%

“…Leishmaniases are diseases caused by protozoans from the genus Leishmania (Kinetoplastida: Trypanosomatidae). Clinically and epidemiologically, there are three forms of leishmaniasis, each species specific: visceral, cutaneous, and mucocutaneous (Ecco et al 2000, Fighera et al 2003, McAdam et al 2015. The protozoan is transmitted by sandfly vectors (Diptera: Phlebotominae) when females take a blood meal from the vertebrate host (Paranaiba et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Outbreak of leishmaniasis caused by Leishmania enriettii in guinea pigs (Cavia porcellus)

Ribeiro

Koubiach²,

Schuler‐Faccini

et al. 2023

Pesq. Vet. Bras.

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We describe an outbreak of leishmaniasis in seven guinea pigs (Cavia porcellus) in which nodular ulcerated skin lesions of varying sizes were observed in the nasal cavity, upper lip, pinnae, vulva, and periarticular region of the limbs. Cytologic exam of collected samples of the lesions in the auricle of one of the animals revealed macrophages containing parasitophorous vacuoles of approximately 4.0μm in diameter in their cytoplasm with morphology suggestive of Leishmania sp. Although skin lesions spontaneously regressed in two of the Guinea pigs, only one survived. All six animals that died were necropsied. Grossly, all animals showed bloody nodular cutaneous lesions with crusts. One of the guinea pigs had distended dark red and firm lungs. Histopathology of the skin lesions revealed histiocytic interstitial acanthotic dermatitis associated with a myriad of Leishmania organisms within macrophages cytoplasm. In the lung, the lesions were characteristic of broncho-interstitial pneumonia with focal infiltrates of neutrophils, epithelioid macrophages, and multinucleated giant cells containing 2µm basophilic amastigotes with morphology compatible with Leishmania spp. A focal granulomatous lesion ,associated with the causal agent in the lung is a novel description of leishmaniasis in guinea pigs caused by L. enriettii. The polymerase chain reaction (PCR) technique with mini-exon primer performed in samples of lesions from two affected guinea pigs was positive and equal to the reference strain, identifying Leishmania enriettii. The cytological, macroscopic, and histological lesions associated with the PCR technique allowed the diagnosis of leishmaniasis and the identification of the specie L. enriettii.

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“…Although there is currently no concrete evidence of natural L. i. chagasi infection in cavies, cutaneous lesions attributed to leishmanias have been recorded in another cavy species (Cavia porcellus), without identification of the species of Leishmania (Ecco et al 2000) or have been identified and attributed to Leishmania enrietti (Medina 1946, Muniz & Medina 1948, Machado et al 1994.…”

Section: Discussionmentioning

confidence: 99%

Experimental infection parameters in Galea spixii (Rodentia: Caviidae) with Leishmania infantum chagasi

Barbosa

Queiroz

Jerônimo³

et al. 2008

Mem. Inst. Oswaldo Cruz

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Leishmaniose cutânea em cobaias (Cavia porcellus)

Cited by 5 publications

References 5 publications

Salivary Gland Extract Modulates the Infection of Two Leishmania enriettii Strains by Interfering With Macrophage Differentiation in the Model of Cavia porcellus

Salivary Gland Extract Modulates the Infection of Two Leishmania enriettii Strains by Interfering With Macrophage Differentiation in the Model of Cavia porcellus

Outbreak of leishmaniasis caused by Leishmania enriettii in guinea pigs (Cavia porcellus)

Experimental infection parameters in Galea spixii (Rodentia: Caviidae) with Leishmania infantum chagasi

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