Leishmaniose visceral (Calazar): cinco casos em cães de Santa Maria, Rio Grande do Sul, Brasil

Pocai, Emersson Augusto; Frozza, Luciano; Headley, Selwyn Arlington; Graça, Dominguita Lühers

doi:10.1590/s0103-84781998000300025

Cited by 23 publications

(12 citation statements)

References 11 publications

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“…The clinical signs described in dogs with rangeliosis include sadness, fever, apathy and prostration (PESTANA, 1910a;CARINI & MACIEL, 1914;BRAGA, 1935;POCAI et al, 1998). Jaundice and anemia are also frequently observed.…”

Section: Introductionmentioning

confidence: 99%

Clinical and hematological evaluation of Rangelia vitalii-naturally infected dogs in southeastern Brazil

Lemos

Toma

Assad

et al. 2017

Rev. Bras. Parasitol. Vet.

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Rangelia vitalii, a tick-borne piroplasm that infects dogs, has been recently molecularly characterized in Brazil, Uruguay and Argentina. Studies on molecular characterization of these piroplasms in different Brazilian regions are scarce. The aim of this study was to evaluate clinical and hematological changes in dogs caused by R. vitalii in the mountainous region of the state of Rio de Janeiro. Blood samples from 36 dogs were evaluated for piroplasms and hematological disorders using light microscopy and molecular analysis. Blood samples from all the animals included in this study were confirmed to be positive for R. vitalii through genetic sequencing. Clinical signspresented by 24 of the 36 dogs of the study were evaluated during appointments or hospitalization within private practice. The most frequent clinical disorders in these dogs that were naturally infected with R. vitalii were fever, spontaneous cutaneous bleeding and diarrhea. Normochromic non-regenerative anemia and thrombocytopenia were the most common hematological disorders in these R. vitalii-positive dogs and therefore should be considered in hematological evaluations on suspected cases.Keywords: Piroplasm, Rangelia vitalii, dogs, PCR, hematological evaluation. ResumoRangelia vitalii, um piroplasma transmitido por carrapatos que infecta cães, foi sendo recentemente caracterizado molecularmente no Brasil, Uruguai e Argentina. Nas diferentes regiões brasileiras são escassos os estudos acerca da caracterização molecular destes piroplasmídeos. O objetivo deste estudo foi avaliar as alterações clínicas e hematológicas em cães causadas por R. vitalii na região serrana do Rio de Janeiro. Amostras de sangue total de 36 cães foram examinadas quanto à presença de piroplasmas pela microscopia de luz, alterações hematológicas e análise molecular. Todos os cães do presente estudo foram positivos para R. vitalii através do sequenciamento genético. Dos 36 animais positivos para R. vitalii, 24 foram avaliados clinicamente. As alterações mais frequentemente observadas foram febre, sangramento cutâneo espontâneo e diarréia. Anemia normocítica normocrômica arregenerativa e trombocitopenia foram as alterações hematológicas mais observadas em cães positivos para R. vitalii, devendo ser consideradas na avaliação hematológica de cães suspeitos.

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Section: Introductionmentioning

confidence: 99%

Clinical and hematological evaluation of Rangelia vitalii-naturally infected dogs in southeastern Brazil

Lemos

Toma

Assad

et al. 2017

Rev. Bras. Parasitol. Vet.

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“…At present, VL is also spreading to other urban areas in the southern and southeast regions, where the disease was not previously endemic. 31 In the infectious cycle of VL, the dog is the most important domestic reservoir and exhibits chronic evolution of the disease. 1,8,21 Leishmania is an obligatory intracellular parasite of mononuclear phagocytes.…”

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confidence: 99%

Histopathologic Patterns of Nephropathy in Naturally Acquired Canine Visceral Leishmaniasis

et al. 2003

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Abstract. Although the nephropathy of visceral leishmaniasis (VL) is known both in humans and dogs, histopathologic alterations have not been thoroughly studied. We examined renal alterations in 55 dogs with naturally acquired VL compared with five noninfected dogs from an endemic area in northeastern Brazil. Glomerulonephritis was found in 55 dogs, interstitial alterations in 53 dogs, and tubular changes in 43 dogs with VL. The glomerular alterations found were minor glomerular abnormalities (n ϭ 8, 14.5%), focal segmental glomerulosclerosis (n ϭ 10, 18.2%), mesangial proliferative glomerulonephritis (n ϭ 17, 32.7%), membranoproliferative glomerulonephritis ,(n ϭ 18, 30.9%), crescentic glomerulonephritis (n ϭ 1, 1.8%), and chronic glomerulonephritis (n ϭ 1, 1.8%). Morphometric and ultrastructural studies complemented the analysis. The five control animals exhibited no glomerular alterations. The glomerular lesions were related to functional alterations. Considering that the alterations of canine and human nephropathy in VL are very similar, the data obtained in this study constitute an important contribution to the understanding of canine and human VL nephropathy.Key words: Dogs; glomerulonephritis; morphometry; renal pathology; ultrastructure; visceral leishmaniasis.Canine visceral leishmaniasis (VL) is a highly prevalent infection encountered throughout the world. In Brazil, VL results from infection by the protozoan Leishmania (Leishmania) chagasi, which occurs in 18 of 27 Brazilian states. 20,22,26 VL is endemic to northeastern Brazil, and in Teresina, in the State of Piaui, some 1,600 dogs presenting naturally acquired VL were known when this study was initiated. At present, VL is also spreading to other urban areas in the southern and southeast regions, where the disease was not previously endemic. 31 In the infectious cycle of VL, the dog is the most important domestic reservoir and exhibits chronic evolution of the disease. 1,8,21 Leishmania is an obligatory intracellular parasite of mononuclear phagocytes. During infection, various host organs are affected including the kidney. Although VL-related nephropathy is known both in humans and dogs, the histopathologic patterns of the lesions present in VL have not been clearly established because most studies are based on very few cases and mainly because well-defined, lesion classification criteria were not used. 3,4,9,10,24,37,38 In human VL, glomerulosclerosis, mesangial cell proliferation, and interstitial nephritis have been reported. 3,9,10,16,18,38 Renal involvement in canine VL is also frequent, and the renal changes are similar to those seen in humans. 2,5,8,23,32 This similarity renders the study of canine VL nephropathy of interest with regard to human pathology. The renal lesion itself does not lead to renal insufficiency. However, when moderate or severe renal lesions are present, VL patients with systemic complications such as secondary infections, sepsis, and hypotension do develop renal insufficiency. 16 Furthermore, glomerular lesions appare...

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“…From a physiopathological standpoint, the interstitial nephritis found here was an important lesion, considering that altered kidney function is more related to interstitial than to glomerular alterations (BOHLE et al, 1987). The kidney conditions found in the present study, particularly diffuse membranoproliferative glomerulonephritis and interstitial nephritis, may lead to kidney failure, which is considered to be the main cause of death among animals with CVL (POCAI et al, 1998).…”

Section: Introductionmentioning

confidence: 60%

“…Moreover, a secondary inflammatory reaction, decreased perfusion of the peritubular capillaries and tubular and interstitial tissue ischemia can be seen (LOPEZ et al, 1996). This condition may be found in both symptomatic (POLI et al, 1991) and asymptomatic dogs (CIARAMELLA et al, 1997), and is the main cause of death among these animals (LOPEZ et al, 1996;POCAI et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Histological changes and immunolabeling ofLeishmania infantum in kidneys and urinary bladder of dogs

Santos

Alves

Ramos

et al. 2013

Rev. Bras. Parasitol. Vet.

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The aim of this study was to assess histological alterations and perform immunolabeling of Leishmania infantum in the kidneys and urinary bladder of naturally infected dogs. Twenty-five urinary bladder and kidney samples of serologically positive animals (ELISA S7® Biogene and IFAT ≥ 1:40 -Biomanguinhos/Fiocruz) were analyzed by means of immunohistochemical and histological techniques. Cystitis was found in 44% (11/25) of the bladder samples and membranoproliferative glomerulonephritis in 92% (23/25) of the kidney samples. Immunolabeling of the parasite revealed that 32% (8/25) of the bladders and 8% (2/25) of the kidneys were positive. In conclusion, the immunohistochemical technique is a useful tool for detecting amastigote forms of L. infantum in organs of infected dogs. In addition, this was the first report of detection of amastigote forms of L. infantum in the bladders of dogs.Keywords: Leishmania infantum, immunohistochemistry, nephropathy, cystitis, dog. ResumoObjetivou-se neste estudo avaliar as alterações histológicas e realizar a imunomarcação de Leishmania infantum em rins e bexiga de cães naturalmente infectados. Vinte e cinco amostras de bexiga e rins de animais sorologicamente (ELISA S7® Biogene and IFAT ≥ 1:40 -Biomanguinhos/Fiocruz) positivas foram analisadas histologicamente e por meio da técnica de imuno-histoquímica. Os resultados revelaram cistite em 44% (11/25) das amostras de bexiga e glomerulonefrite membranoproliferativa em 92% (23/25) das amostras de rins. A imunomarcação do parasito revelou 32% (8/25) e 8% (2/25) de positividade em bexiga e rins, respectivamente. Conclui-se que a técnica de imunohistoquímica é uma útil ferramenta para detecção de formas amastigotas de L. infantum em órgãos de cães infectados. Além disso, o presente trabalho reporta a primeira descrição de formas amastigotas de L. infantum em bexiga de cães.

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Leishmaniose visceral (Calazar): cinco casos em cães de Santa Maria, Rio Grande do Sul, Brasil

Cited by 23 publications

References 11 publications

Clinical and hematological evaluation of Rangelia vitalii-naturally infected dogs in southeastern Brazil

Clinical and hematological evaluation of Rangelia vitalii-naturally infected dogs in southeastern Brazil

Histopathologic Patterns of Nephropathy in Naturally Acquired Canine Visceral Leishmaniasis

Histological changes and immunolabeling ofLeishmania infantum in kidneys and urinary bladder of dogs

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