Glutaraldehyde release from heat-polymerized acrylic resins after disinfection and chemical and mechanical polishing

Abstract

This study evaluated the release of glutaraldehyde from heat-polymerized acrylic resins subjected to disinfection followed by chemical and mechanical polishing. Ninety disc-shaped specimens (15 x 4 mm), 30 per resin (Lucitone 550, QC-20 and Classico), were made and assigned to 2 groups according to the type of polishing. One side of each specimen was not polished and the other was either mechanically (n = 45) or chemically (n = 45) polished, and immersed in water at 50 °C for 1 h to allow the release of intrinsic substances and then kept in distilled water for 7 days. The specimens were disinfected by immersion in 2% glutaraldehyde for 10 min. After this period, 3 specimens from each group were immersed in water for 15, 30, 60, 120 and 240 min. For the 15-, 30-, 60-min immersions, 4 water exchanges were done at the end of period. High performance liquid chromatography (HPLC) was used to detect and quantify the glutaraldehyde released after each period. Data were analyzed statistically by two-way ANOVA and multiple comparisons were done by Tukey's and Scheffé's tests (α = 0.05). No glutaraldehyde release was observed from the specimens with chemical polishing at any of the immersion periods, while the mechanically polished specimens released glutaraldehyde. In the groups with water exchanges, Lucitone released more disinfectant in the 15-min period (0.040 μg/mL), Classico in the 30-min (0.021 μg/mL) and 60-min (0.018 μg/mL) periods, and QC-20 the same amount (-1.760 μg/mL) in all periods. In the groups without water exchanges, Lucitone released the highest amount of disinfectant (-1.370 μg/mL), differing significantly from QC-20 (0022 g/mL) and Classico (0019 g/mL), which were similar. The findings of this showed that chemically polished specimens from the 3 resin brands did not release glutaraldehyde after different periods of immersion, while glutaraldehyde release was observed from the mechanically polished specimens, especially from those made of Lucitone resin.

Effect of sodium hypochlorite and peracetic acid on the surface roughness of acrylic resin polymerized by heated water for short and long cycles

“…Both NaClO and C 2 H 4 O 3 are recommended by the American Dental Association, where NaClO can be used to disinfect acrylic resin dentures and C 2 H 4 O 3 for cleaning and disinfecting, as alternatives to the chemical disinfectant glutaraldehyde, which can be highly toxic. [ 19 ] C 2 H 4 O 3 is a more powerful oxidant than chloride or chloride dioxide, causing the rupture of the cell membrane by means of protein denaturing. [ 13 ] Moreover, C 2 H 4 O 3 can disinfect specimens contaminated with Bacillus subtilis and Bacillus stearothermophilus .…”

Surface detail reproduction and dimensional accuracy of stone models: influence of disinfectant solutions and alginate impression materials

“…Disinfection of tools and materials is an important step in dental procedures (1)(2)(3). In the absence of disinfection, treatment procedures can expose dentists, hygienists and laboratory workers to direct or crosscontamination (4,5).…”

Cytotoxic potential of denture base and reline acrylic resins after immersion in disinfectant solutions

“…One mechanism is that absorbed agents from disinfectants are released and negatively impact the cells. 49 The other mechanism is that degradation of the acrylic resins releases toxic byproducts. 32 In addition, it is possible that an interaction of both mechanisms has occurred.…”